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Study on the Elimination Method of Small Rounded Structure Virus (SRSV) from Oyster.

牡蛎小圆结构病毒(SRSV)消除方法的研究。

基本信息

批准号：
14360102
负责人：
YOSHIMIZU Mamoru
金额：
$ 7.68万
依托单位：
Hokkaido University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2004
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14360102/
关键词：
Oyster Norovirus SRSV Elimination Electryzed seawater UV irradiation Shell remove FCV 牡蠣 SRSV 電解殺菌加圧牡蛎大腸菌海水電気分解電解水紫外線

项目摘要

Oysters (Crassestrea gigas) harvested from major cultivation areas are commonly contaminated with Escherichia coli. Also, some places, oyster concentrates small rounded structured virus (SRSV ; norovirus). It's very important to remove the E.coli, food born bacteria and norovirus. E.coli has to be removed from oysters during oyster purification by re-laying in a non-polluted seawater. Although sodium hypochlorite or UV irradiation is used to disinfect seawater, it's difficult to treat large volumes of seawater. Recently the bactericidal and virucidal effects of hypochlorite produced by electrolysis of seawater were examined against pathogenic bacteria and viruses.SRSV (norovirus) is not able to replicate in the established cell line, and its difficult to determine the virus infectivity. This virus is belonging to family Caliciviridae, and cat pathogenic virus ; FCV that is able to replicate in cat cell line. In this study, virucidal effects of UV irradiation, hypochlorite and high pres … More sure on FCV infectivity were studied to eliminate the FCV from oyster. Stability of FCV in sea water and intestinal contents of oyster was also studied.FCV was inactivated at the dose of 1.0x10^4 micro W・sec/cm^2, electrolyzed seawater chlorine concentration controlled at 0.20〜0.4mg/L, and 800MPa・40℃・5min FCV infectivity decreased rapidly in sea water at the temperature 20℃ or high. In the intestinal contents of oyster, FCV was inactivated by antiviral substances produced by intestinal bacteria. These results indicate that combination of 3 conditions ; (1)using a electrolyzed sea water containing the 0.3 mg/L hypochlorite, (2)harvest in sea water at the temperature 20℃ or high, (3)remove the shell using a 800 MPa・at 40℃ for 5 min. Seawater electrolyzers have ability to treat large volumes of water and cost performance of this method is better than that of UV irradiation. From these results, it is considered that electrolysis of seawater could be a useful method for post harvest elimination of norovirus from oysters. Less

从主要养殖区收获的牡蛎（Crassestrea gigas）通常被大肠杆菌污染。此外，有些地方，牡蛎浓缩小圆形结构病毒（SRSV ;诺如病毒）。这是非常重要的，以消除大肠杆菌，食物传播的细菌和诺如病毒。在牡蛎净化过程中，必须通过重新放置在未受污染的海水中来去除牡蛎中的大肠杆菌。虽然使用次氯酸钠或紫外线照射来消毒海水，但很难处理大量海水。近年来，人们研究了电解海水产生的次氯酸盐对病原菌和病毒的杀菌和杀病毒作用。SRSV（诺如病毒）不能在已建立的细胞系中复制，其感染性难以确定。该病毒属于杯状病毒科，猫致病性病毒;能够在猫细胞系中复制的FCV。本研究比较了紫外线照射、次氯酸盐和高压灭菌对病毒的杀灭效果， ...更多信息为消除牡蛎中的FCV，对FCV的感染性进行了研究。在1.0 × 10^4 μ W·sec/cm ^2剂量、控制电解海水氯浓度为0.20 ~ 0.4mg/L、800 MPa·40℃·5 min条件下，FCV在20℃以上海水中的感染力迅速下降。在牡蛎的肠道内容物中，FCV被肠道细菌产生的抗病毒物质灭活。结果表明：（1）采用含0.3 mg/L次氯酸盐的电解海水，（2）在20℃或更高温度下收获，（3）在40℃下用800 MPa·5 min去除贝壳。海水电解槽具有处理大体积水的能力，该方法的性价比优于紫外线照射。从这些结果，它被认为是电解海水可能是一个有用的方法，收获后消除诺如病毒从牡蛎。少