Study on the Elimination Method of Small Rounded Structure Virus (SRSV) from Oyster.

Oysters （Crassestrea gigas）从主要培养区域被广泛地控制。此外， some places， oyster concentrates small rounded structured virus （SRSV ; norovirus）。这对消除大肠杆菌、食物出生的细菌和正常病毒非常重要。E.大肠杆菌将被从牡蛎净化后从一个非污染的海鲜中移除。Although钠hypochlorite或紫外线辐射被用于干扰的海员，这是很难处理的海员的大体积。最近通过测试近海病原体和病毒的电泳产生的高氯丁胺的细菌和病毒效应，SRSV（Norovirus）不能在已稳定的细胞线上复制，并且它难以确定病毒感染。这种病毒属于Caliciviridae家族和猫病理病毒; FCV可以在猫细胞中复制。在本研究中，紫外线辐射、高氯离子和高污染的病毒效应 ... More 关于FCV感染的研究表明，FCV从牡蛎中消除了FCV。FCV in sea water and intestinal contents of oyster was also studied. FCV was inactivated at the dose of 1.0 x10 ^4 micro W·sec/cm^2， electrolyzed seawater chlorine concentration controlled at 0.20 ~ 0.4 mg/L， and 800 MPa·40℃·5 min FCV infectivity decreased rapidly in sea water at the temperature 20℃或高。在牡蛎的内脏内容中， FCV被内脏细菌产生的非病毒物质激活。这些结果表明了3种条件的组合; （1）使用电泳的海水含有0.3毫克/升的高氯石;（2）在20℃或较高的温度下收获海洋水;（3）将外壳使用800兆帕·在40℃下5分钟。海水电致器有能力处理较大的水体积和成本性能的这种方法比紫外线辐射要好。从这些结果中可以看出，海鸥的电致作用可能是牡蛎后的诺维病毒的有效方法。Less（低）

Oysters (Crassestrea gigas) harvested from major cultivation areas are commonly contaminated with Escherichia coli. Also, some places, oyster concentrates small rounded structured virus (SRSV ; norovirus). It's very important to remove the E.coli, food born bacteria and norovirus. E.coli has to be removed from oysters during oyster purification by re-laying in a non-polluted seawater. Although sodium hypochlorite or UV irradiation is used to disinfect seawater, it's difficult to treat large volumes of seawater. Recently the bactericidal and virucidal effects of hypochlorite produced by electrolysis of seawater were examined against pathogenic bacteria and viruses.SRSV (norovirus) is not able to replicate in the established cell line, and its difficult to determine the virus infectivity. This virus is belonging to family Caliciviridae, and cat pathogenic virus ; FCV that is able to replicate in cat cell line. In this study, virucidal effects of UV irradiation, hypochlorite and high pres … More sure on FCV infectivity were studied to eliminate the FCV from oyster. Stability of FCV in sea water and intestinal contents of oyster was also studied.FCV was inactivated at the dose of 1.0x10^4 micro W・sec/cm^2, electrolyzed seawater chlorine concentration controlled at 0.20〜0.4mg/L, and 800MPa・40℃・5min FCV infectivity decreased rapidly in sea water at the temperature 20℃ or high. In the intestinal contents of oyster, FCV was inactivated by antiviral substances produced by intestinal bacteria. These results indicate that combination of 3 conditions ; (1)using a electrolyzed sea water containing the 0.3 mg/L hypochlorite, (2)harvest in sea water at the temperature 20℃ or high, (3)remove the shell using a 800 MPa・at 40℃ for 5 min. Seawater electrolyzers have ability to treat large volumes of water and cost performance of this method is better than that of UV irradiation. From these results, it is considered that electrolysis of seawater could be a useful method for post harvest elimination of norovirus from oysters. Less