Practical use of RNA vaccine at aquaculture field.

在这项研究中，我们用合成的双链RNA[Poly(I：C)]和活的致病病毒建立了一种新型的鱼类免疫。用活的致病病毒免疫的鱼，然后用Poly(I：C)免疫，成为一种短暂的、非特异性的抗病毒状态，这些鱼在最初的活病毒免疫后存活下来，从而对注射的致病病毒产生特异性保护性免疫反应。分别用IHNV、VHSV和NNV进行Poly(I：C)免疫，对虹鱼、牙鲆和七带石斑鱼均有较强的保护性免疫应答。可以想象，Poly(I：C)免疫最终将适用于广泛的鱼类物种以及其他病毒。

In this study, we developed a novel fish-immunization with a synthetic double-stranded RNA [Poly(I:C)] and live pathogenic viruses. The fishes immunized with live pathogenic virus, followed by administration of Poly(I:C), became a transient, non-specific antiviral state, and those fish survive the initial immunization with live virus, thereby mounting a specific protective immune response against the injected pathogenic virus. Strong protective immune response was observed in rainbow trout, Japanese flounder and sevenband grouper by Poly(I:C)-immunization with IHNV, VHSV and NNV, respectively. It is conceivable that Poly(I:C)-immunization will eventually be applicable to a wide range of fish species as well as other viruses.