Practical use of RNA vaccine at aquaculture field.

RNA疫苗在水产养殖领域的实际应用。

• 批准号: 21380112
• 负责人: YOSHIMIZU Mamoru
• 金额: $ 11.07万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21380112/
• 关键词: 魚病; ウイルス; ワクチン; ds-RNA; Poly(I:C); インターフェロン; 免疫

In this study, we developed a novel fish-immunization with a synthetic double-stranded RNA [Poly(I:C)] and live pathogenic viruses. The fishes immunized with live pathogenic virus, followed by administration of Poly(I:C), became a transient, non-specific antiviral state, and those fish survive the initial immunization with live virus, thereby mounting a specific protective immune response against the injected pathogenic virus. Strong protective immune response was observed in rainbow trout, Japanese flounder and sevenband grouper by Poly(I:C)-immunization with IHNV, VHSV and NNV, respectively. It is conceivable that Poly(I:C)-immunization will eventually be applicable to a wide range of fish species as well as other viruses.

本研究利用合成的双链RNA [Poly(I:C)]和活的致病性病毒，开发了一种新的鱼免疫方法。用活的致病病毒免疫的鱼，随后给予Poly(I:C)，成为一种短暂的、非特异性的抗病毒状态，这些鱼在最初的活病毒免疫中存活下来，从而对注射的致病病毒产生特异性的保护性免疫反应。虹鳟鱼、日本比目鱼和七带石斑鱼分别接种IHNV、VHSV和NNV的Poly（I:C）免疫后出现了较强的保护性免疫应答。可以想象，Poly(I:C)-免疫最终将适用于广泛的鱼类物种以及其他病毒。

项目成果

Protection of rainbow trout from infectious hematopoietic necrosis (IHN) by injection of infectious pancreatic necrosis virus (IPNV) or Poly(I:C)

• DOI: 10.3354/dao02000
• 发表时间: 2009-02-12
• 期刊: DISEASES OF AQUATIC ORGANISMS
• 影响因子: 1.4
• 作者: Kim, Hyoung Jun;Oseko, Norihisa;Yoshimizu, Mamoru
• 通讯作者: Yoshimizu, Mamoru

サケ科魚ヘルペスウイルスOncorhynchus masou virus (OMV) の全ゲノム解析

鲑鱼疱疹病毒 Oncorhynchus masou 病毒 (OMV) 的全基因组分析

• 发表时间: 2013
• 作者: 篠原 慶規;平岡 真合乃;五味 高志;笠井久会・吉水 守
• 通讯作者: 笠井久会・吉水 守

Survivability of koi herpesvirus(KHV) and a new approach for disinfection of KHV in fish breeding effluent water using anti-KHV bacteria

锦鲤疱疹病毒（KHV）的生存能力以及利用抗KHV细菌对养鱼废水中KHV进行消毒的新方法

• 发表时间: 2011
• 作者: Yoshida;N.;H. Kasai and M. Yoshimizu
• 通讯作者: H. Kasai and M. Yoshimizu

Detection methods for epizootiological survey of Aeromonas salmonicida, the causative agent of furunculosis

疖病病原杀鲑气单胞菌流行病学调查的检测方法

• 发表时间: 2011
• 作者: Devon Dublin;et al.
• 通讯作者: et al.

Survivability of koi Herpesvirus (REV) and a new approach for disinfection of KHV in fish breeding effluent water using anti-KHV bacteria.

锦鲤疱疹病毒 (REV) 的生存能力以及使用抗 KHV 细菌对鱼类养殖废水中 KHV 进行消毒的新方法。

• 发表时间: 2010
• 作者: Yoshida;N.;et al.
• 通讯作者: et al.