Enhancement of EPA and DHA biosynthesis by over-expression of fatty acid metabolic enzymes in marine fish

通过过度表达海水鱼类脂肪酸代谢酶来增强 EPA 和 DHA 生物合成

• 批准号: 14360108
• 负责人: KIRON Viswanas
• 金额: $ 7.94万
• 依托单位: Tokyo University of Marine Science and Technology (2004)東京水産大学 (2002-2003)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14360108/
• 关键词: fatty acid desaturase; transgenic fish; zebrafish; docosahexaenoic acid; eicosapentaenoic acid; gene transfer; healthy food; 栄養強化; ゼブラフィツシュ; 脂肪酸Δ6不飽和化酵素; cDNA; ヤマメ

The n-3 polyunsaturated fatty acids, especially eicosapentaenoic acid(EPA) and docosahexaenoic acid(DHA) have important nutritional benefits in humans. Farmed fish could serve as promising sources of EPA/DHA, but they need these fatty acids or their precursors in their diets. Here we transferred masu salmon Δ6-desaturase-like gene in zebrafish to increase its ability for synthesizing EPA and DHA. Expression of this gene in transgenic fish elevated their EPA content by 1.4 fold and DHA by 2.1 fold. On the other hand, the α-linolenic acid content decreased, it being a substrate of Δ6-desaturase, while the total lipid remained constant. This achievement demonstrates that fatty acid metabolic pathway in fish can be modified by the transgenic technique, and perhaps this could be applied to tailor farmed fish as even better sources of valuable human food.

N-3多不饱和脂肪酸，特别是二十碳五烯酸(EPA)和二十二碳六烯酸(DHA)对人体有重要的营养价值。养殖鱼可以作为EPA/DHA的潜在来源，但它们在饮食中需要这些脂肪酸或其前体。为了提高斑马鱼合成Δ和DHA的能力，我们将马哈鱼的6-脱氢酶基因转入斑马鱼体内。该基因的表达使转基因鱼的EPA含量提高了1.4倍，DHA含量提高了2.1倍。α-亚麻酸作为Δ6-去饱和酶的底物，其含量降低，而总脂含量保持不变。这一成果表明，转基因技术可以改变鱼类体内的脂肪酸代谢途径，或许可以应用于定制养殖的鱼类，作为更好的人类宝贵食物来源。

项目成果

Enhancement of EPA and DHA biosynthesis by over-expression of masu salmon Δ6-desaturase-like gene in zebrafish

• DOI: 10.1007/s11248-004-7435-7
• 发表时间: 2005-04-01
• 期刊: TRANSGENIC RESEARCH
• 影响因子: 3
• 作者: Alimuddin;Yoshizaki, G;Takeuchi, T
• 通讯作者: Takeuchi, T

Enhancement of EPA and DHA biosynthesis by over-expression of masou salmon Δ6-desaturase-like gene in zebrafish.

通过在斑马鱼中过度表达 masou 鲑鱼 Δ6-去饱和酶样基因增强 EPA 和 DHA 生物合成。

• 发表时间: 2005
• 期刊: Transgenic Research 14
• 作者: Aliniudin;Yoshizaki;G.;Kiron;V.;Satoh;S.;Takeuchi;T.
• 通讯作者: T.

不飽和脂肪酸含量の増加したトランスジェニック魚類

不饱和脂肪酸含量增加的转基因鱼

• 发表时间: 2004

吉崎悟朗: "魚類への外来遺伝子導入による新たな育種に関する研究"日本水産学会誌. 68. 500-503 (2002)

吉崎五郎：“将外源基因引入鱼类的新育种研究”日本水产学会杂志 68. 500-503 (2002)。