The Study of Mechanisms of Aflatoxin Detoxification in Animals

动物黄曲霉毒素解毒机制的研究

• 批准号: 14360185
• 负责人: KUMAGAI Susumu
• 金额: $ 9.54万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14360185/
• 关键词: aflatoxin; glutathione-S-transferase; mastomys; hamster; mouse; rat; グルタチオントランスフェラーゼ; アルデヒド; リダクターゼ

In order to study the glutathione-S-transferase (GST) activity toward aflatoxin BI (AFBI) epoxide in rodents, we performed in vitro studies of the cytosolic GST activity using mastomys, rat, mouse and hamster liver. AFBI, glutathione and co-enzymes were incubated with the liver cytosol fraction in the presence of the hamster microsomes, which were used to generate AFBI-epoxide as a substrate for GST, and then AFBI-glutathione conjugate formed was analyzed by HPLC. The result demonstrated that GST activity was highest in mastomys liver, and higher in the hamster and mouse livers than in the rat liver, correlating with the difference of the sensitivity of these species to the AFBI toxicity. Sex differences were noted in the rat, the female activity being higher than the male activity. Comparison of cytosolic GST activity among different organs in these species revealed that the CST activity was higher in the liver than in the small intestine, kidney, testis, lung and brain in the mastomys, hamster, maouse and female rat. In the male rat, the small intestine showed the highest activity among the organs. The AFBI aldehyde reductase activity in the liver cytosol was also measured by determining metabolic conversion of AFBI-aldehyde to AFBI-dialcohol and compared among different animal species.

为了研究啮齿动物中谷胱甘肽-S-转移酶 (GST) 对黄曲霉毒素 BI (AFBI) 环氧化物的活性，我们使用乳腺、大鼠、小鼠和仓鼠肝脏进行了细胞质 GST 活性的体外研究。在仓鼠微粒体存在下，将 AFBI、谷胱甘肽和辅酶与肝细胞溶质级分一起孵育，用于生成 AFBI-环氧化物作为 GST 的底物，然后通过 HPLC 分析形成的 AFBI-谷胱甘肽缀合物。结果表明，乳鼠肝脏中的 GST 活性最高，仓鼠和小鼠肝脏中的 GST 活性高于大鼠肝脏，这与这些物种对 AFBI 毒性的敏感性差异相关。在大鼠中注意到性别差异，雌性活动高于雄性活动。比较这些物种不同器官的胞质 GST 活性表明，在乳腺、仓鼠、毛鼠和雌性大鼠中，肝脏中的 CST 活性高于小肠、肾、睾丸、肺和脑。在雄性大鼠中，小肠在器官中表现出最高的活性。还通过测定 AFBI-醛向 AFBI-二醇的代谢转化来测量肝细胞质中的 AFBI 醛还原酶活性，并在不同动物物种之间进行比较。

项目成果

Poapolathep A, Sugita-Konishi Y, Doi K, Kumagai S.: "The fates of trichothecene mycotoxins, nivalenol and fusarenon-X, in mice."Toxicon. 41. 1047-1054 (2003)

Poapolathep A、Sugita-Konishi Y、Doi K、Kumagai S.：“单端孢菌素真菌毒素、雪腐镰刀菌烯醇和 Fusarenon-X 在小鼠体内的命运。”Toxicon。

Poapolathep, Kumagai, et al.: "The fates of trichothecene mycotoxins, nivalenol and fusarenon-X, in mice"Toxicon. 41・8. 1047-1054 (2003)

Poapolathep、Kumagai 等人：“单端孢菌素真菌毒素、雪腐镰刀菌烯醇和 Fusarenon-X 在小鼠中的命运”Toxicon 41·8 (2003)。

Bintvihok A, Thiengnin S, Doi K, Kumagai S.: "Residues of aflatoxins in the liver, muscle and eggs of domestic fowls."J Vet Med Sci. 64. 1037-1039 (2002)

Bintvihok A, Thiengnin S, Doi K, Kumagai S.：“家禽肝脏、肌肉和鸡蛋中黄曲霉毒素的残留。”J Vet Med Sci。

Poapolathep, Kumagai, et al.: "Development of early apoptosis and changes in lymphocyte Subsets in lymphoid organs of mice orally inoculated with nivalenol."Exp.Mol.Pathol.. 75・1. 74-79 (2003)

Poapolathep、Kumagai 等人：“口服雪腐镰刀菌醇的小鼠淋巴器官中早期细胞凋亡的发展和变化。”Exp.Mol.Pathol.. 75・1 (2003)。

Bintvihok, Kumagai, et al.: "Aflatoxin Contamination in Shrimp feed and Effects of aflatoxin addition to feed on shrimp production."J.Food Prot.. 66・5. 882-885 (2003)

Bintvihok、Kumagai 等人：“虾饲料中的黄曲霉毒素污染以及饲料中添加黄曲霉毒素对虾生产的影响”。J.Food Prot. 66・5 (2003)。