The study of the effect of natural contamination of feed with mycotoxins on the occurrence of diseases in farm animals.

研究霉菌毒素饲料的自然污染对农场动物疾病发生的影响。

• 批准号: 15405039
• 负责人: KUMAGAI Susumu
• 金额: $ 9.09万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15405039/
• 关键词: aflatoxin; Thai; Farm animals; Feed contamination; zearalenon; かび毒; 毒性; 飼料; ブタ; タイ国; カビ毒

(1) In 2000-2004, aflatoxin B1 (AFB1), zearalenon (ZEA) and deoxynivalenol contamination in feed and its materials were surveyed in Thailand. Among them, cassava was most heavily contaminated with mycotoxins. (2) Analysis of contaminated molds revealed Aspergillus flavus、 A.nigar、. and Penicillium in completed feeds, Aspergillus flavus and A.nigar in corn, Penicillium and Paecilomyces in cassava and Rhizopu in rice bran, as dominant molds. (3) Cassava samples were collected and analyzed for mycotoxin and mold contamination. Aspergillus sp including A.flavus and Fusarium sp were found in a part of the samples, but mycotoxins were detected in most of them, the maximum concentrations of AFB1,AFB2,AFG1,AFG2 and ZEA being 31.3ppb、 18.2ppb、 53.4ppb、 48.5ppb and 189.4ppb, respectively. (4) The study of aflatoxin metabolism in pigs demonstrated that high susceptibility of pigs reflects lower hepatic glutathion S-transferase activity compared with the other animal species, and that the reductase activity is not involved in the species difference of aflatoxin toxicity. (5) Pigs were given the feed containing coumarin- or tea extract for one month, and then toxic effects of a single oral administration of AFB1 in them were studied. Inhibitory effects of the treatment on toxicity and tissue residue of AFB1 were noted slightly in coumarin, but not in tea extract.

（1）2000-2004年，泰国对饲料及其原料中黄曲霉毒素B1（AFB1）、玉米赤霉烯酮（ZEA）和脱氧雪腐镰刀菌烯醇污染情况进行了调查。其中，木薯受霉菌毒素污染最严重。 (2)污染霉菌分析显示为黄曲霉、黑曲霉、。成品饲料中的青霉属、黄曲霉属和黑曲霉属、木薯中的青霉属和拟青霉属以及米糠中的根霉属为主要霉菌。 (3)收集木薯样品并分析霉菌毒素和霉菌污染。部分样品中检出黄曲霉、镰刀菌等曲霉菌，但大部分样品检出霉菌毒素，AFB1、AFB2、AFG1、AFG2、ZEA最高浓度分别为31.3ppb、18.2ppb、53.4ppb、48.5ppb、189.4ppb。 (4)黄曲霉毒素在猪体内的代谢研究表明，猪的高易感性反映了其肝脏谷胱甘肽S-转移酶活性较其他动物较低，且还原酶活性与黄曲霉毒素毒性的种属差异无关。 (5)给猪饲喂含有香豆素或茶提取物的饲料1个月，然后研究单次口服AFB1对猪的毒性作用。在香豆素中注意到处理对 AFB1 的毒性和组织残留有轻微的抑制作用，但在茶提取物中则没有。

项目成果

Occurrence of Aflatoxins, Ochratoxin A and Fumonisins in Retailed Foods in Japan

日本零售食品中黄曲霉毒素、赭曲霉毒素 A 和伏马菌素的存在

• 发表时间: 2006
• 期刊: J.Food Prot. (In press)
• 作者: Suigta-Konishi;Y.;Kumagai;S.;et al.
• 通讯作者: et al.

Occurrence of aflatoxin M1 in domestic milk in Japan during the winter season.

冬季日本国产牛奶中出现黄曲霉毒素M1。

• 发表时间: 2004
• 期刊: Food Add. Contam. 21
• 作者: M.Nakajima;S.Tabata;H.Akiyama;Y.Itoh;T.Tanaka;H.Sunagawa;T.Tyonan;T.Yoshizawa;S.Kumagai
• 通讯作者: S.Kumagai

New Horizon of Mycotoxicology for Assuring Food Satety

确保食品安全的真菌毒理学新视野

• 发表时间: 2004
• 作者: Kumagai S;その他
• 通讯作者: その他

The mechanism of the carcinogenic effect of aflatoxins and the occurrence of Aflatoxin in nuts in Japan.

黄曲霉毒素的致癌作用机制及日本坚果中黄曲霉毒素的发生。

• 发表时间: 2006
• 期刊: Mycotoxins 55(2)
• 作者: Sugita-Konishi;Y. Kumagai;S.
• 通讯作者: S.

Comparative activities of glutathione-S-transferase and dialdehyde reductase toward aflatoxin B1 in livers of experiment and farm animals.

实验动物和家畜肝脏中谷胱甘肽-S-转移酶和二醛还原酶对黄曲霉毒素 B1 的活性比较。

• 发表时间: 2005
• 期刊: Toxicon 46
• 作者: Tulayakul P.;Sakuda S.;Dong K.S.;Kumagai S
• 通讯作者: Kumagai S