Studies on the health effects of exercise

运动对健康影响的研究

• 批准号: 14370140
• 负责人: TAKEUCHI Toru
• 金额: $ 7.49万
• 依托单位: Kagoshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14370140/
• 关键词: Exercise; Reactive Oxygen Species; Oxidative DNA damage; Anaerobic exercise; Aerobic exercies; Swimming; Fe-nitrilotriacetic acid; rat; 酸化ストレス耐性; SOD; 8-hydroxydeoxyguanosine; OGG1; 酸化的損傷; 尿酸; 8-ヒドロキシグアニン

In this study we did the following experiments.We have established a simple method to collect white blood cells from blood using dextran sulfate for the determination of 8-hydroxydeoxyguanosine(8OHdG), one of typical oxidative DNA damage. We have successfully applied this method for epidemiological studies.We studied the difference in effects on oxidative damage between aerobic and anaerobic exercise. Postgraduate students were subjected to anaerobic or aerobic exercise using cycle ergometer. The exercise loads were the same in both exercise groups. Anaerobic exercise increased 8OHdG in white blood cells 24 hours after the exercise. Anaerobic exercise increased serum uric acid, suggesting the generation of superoxide by anaerobic exercise. Aerobic exercise did not increased 8OHdG or uric acid.We studied the effects of habitual exercise on oxidative DNA damage and antioxidant enzymes. Rats were divided into two groups, one was sedentary group and the other was swimming group. Swimming group rats were subjected to swim, i.e. 15 to 60 min per swim, 5 times per week, for 10 weeks. Swimming did not increase 8OHdG in kidney. We injected Fe-nitrilotriacetic acid(Fe-NTA) into both groups to induce oxidative stress in kidney. Fe-NTA increased 8OHdG in kidney of both groups, however, Fe-NTA injection induced less 8OHdG in swimming group than in sedentary group. Swimming increased superoxide dismutase activity in kidney and diaphragm, but it did not increase catalase or glutathione peroxidase activities. Swimming decreased OGG1 protein levels in kidney. The data indicate that habitual exercise increased the resistance against oxidative stress and could be beneficial for cancer prevention.We are trying to establish a method to determine 8OHdG repair activity by electrochemical detection after HPLC separation. We are using a synthetic oligonucleotides containing 8OHdG as a substrate. OGG1 activities as low as 0.01 units could be detected by the method.

本研究主要做了以下几个方面的工作：建立了一种简便的用硫酸葡聚糖提取血液中白色细胞的方法，用于检测典型的DNA氧化损伤8-羟基脱氧鸟苷（8-hydroxydeoxyguanosine，8 OHdG）。我们已经成功地将这种方法应用于流行病学研究，研究了有氧运动和无氧运动对氧化损伤的影响。研究生进行无氧或有氧运动使用自行车功率计。两个运动组的运动负荷相同。无氧运动后24小时白色血细胞中8 OHdG增加。无氧运动使血清尿酸升高，提示无氧运动产生超氧化物。有氧运动不增加8-羟基脱氧葡萄糖或尿酸。我们研究了习惯性运动对DNA氧化损伤和抗氧化酶的影响。将大鼠分为两组，一组为安静组，另一组为游泳组。游泳组大鼠进行游泳训练，每次15 ~ 60 min，每周5次，共10周。游泳不增加肾脏8 OHdG含量。给两组动物注射次氮基三乙酸铁（Fe-NTA）诱导肾脏氧化应激。Fe-NTA可使两组大鼠肾脏8 OHdG含量增加，但游泳组大鼠肾脏8 OHdG含量低于安静组。游泳可增加肾脏和膈肌超氧化物歧化酶活性，但不增加过氧化氢酶和谷胱甘肽过氧化物酶活性。游泳降低肾脏OGG 1蛋白水平。本研究尝试建立一种高效液相色谱分离后电化学检测8 OHdG修复活性的方法。我们使用含有8 OHdG的合成寡核苷酸作为底物。该方法可检测到低至0.01单位的OGG 1活性。

项目成果

Lack of direct involvement of 8-hydroxy-2'-deoxyguanosine in hypoxanthine-guanine phophoribosyltransferase mutagenesis in V79 cells treated with N,N'-bis(2-hydroxy-peroxy-2-methoxyethyl) -1,4,5,8-naphthalenetetracarboxylic-diimide(NP-III) or riboflavin.

在用 N,N-双(2-羟基-过氧-2-甲氧基乙基)-1,4,5,8-处理的 V79 细胞中，次黄嘌呤-鸟嘌呤磷酸核糖基转移酶突变中缺乏 8-羟基-2-脱氧鸟苷的直接参与

• 发表时间: 2002
• 期刊: Jpn.J.Cancer Res. 92
• 作者: Nakjima;M.;Takeuchi;T.;Ogino;K.;Morimoto;K.
• 通讯作者: K.

Nitration and chlorination of folic acid by peroxynitrite and hypochlorous acid, and the selective binding of 10-nitro-folate to folate receptor β.

过氧亚硝酸盐和次氯酸对叶酸进行硝化和氯化，以及 10-硝基叶酸与叶酸受体 β 的选择性结合。

• 发表时间: 2002
• 期刊: Biochem.Biophys.Res.Commun. 297
• 作者: Nakamura;M.;Nagayoshi;R.;Ijiri;K.;Nakashima-Matsushita;N.;Takeuchi;T.;Matsuyama;T.
• 通讯作者: T.

Lack of direct involvement of 8-hydroxy-2'-deoxyguanosine in hypoxanthine-guanine phophoribosyltransferase mutagenesis in V79 cells treated with N,N'-bis(2-hydroxyperoxy-2-methoxyethyl)-1,4,5,8-naphthalenetetracarboxylic-diimide(NP-III) or riboflavin.

在用 N,N-双(2-羟基过氧-2-甲氧基乙基)-1,4,5,8-萘四甲酸-处理的 V79 细胞中，次黄嘌呤-鸟嘌呤磷酸核糖基转移酶突变中缺乏 8-羟基-2-脱氧鸟苷的直接参与

• 发表时间: 2002
• 期刊: Jpn.J.Cancer Res. 92
• 作者: Nakjima;M.;Takeuchi;T.;Ogino;K.;Morimoto;K.
• 通讯作者: K.

Nishibayashi H., et al.: "Helicobacter pylori-induced enlarged-fold gastritis is associated with increased mutagenicity of gastric juice, increased oxidative DNA damage, and an increased risk of gastric carcinoma."J.Gastroenterol.Hepatol.. 18・12. 1384-139

Nishibayashi H.等人：“幽门螺杆菌引起的肥大性胃炎与胃液致突变性增加、氧化DNA损伤增加以及胃癌风险增加有关。”J.Gastroenterol.Hepatol.. 18・12 .1384-139

竹内 亨(分担執筆): "New予防医学・公衆衛生学"編集:岸礼子・古野純典・小泉昭夫・大前和幸、出版:南江堂. 392 (2003)

竹内彻（合著者）：《新预防医学/公共卫生》编辑：岸丽子、古野纯纪、小泉昭夫、大前和幸，出版：Nankodo 392（2003）。