Gene therapy for graft coronary arteriosclerosis targeted at smooth muscle progenitor cells derived from the recipients' bone marrow

针对移植冠状动脉硬化的基因治疗，针对来自受者骨髓的平滑肌祖细胞

• 批准号: 14370407
• 负责人: MORI Yoshio
• 金额: $ 8.77万
• 依托单位: Gifu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14370407/
• 关键词: smooth muscle progenitor cells; graft coronary acteriosclerosis; gene transfer; non-viral vector; 遺伝子導入

The purpose in this study is that whether ox not suppress graft coronary arteriosclerosis using by counter shock method of the DNA coated around the gold particle.DNA vector of the GFP gene was transferred to COS-7 cells in vitro using counter shock method. Then the expression of the GFP gene was confirmed by fluorescent microscopy.In the next study, the donor heart was excised and the GFP gene was transferred into the cardiac graft using counter shock method. Then the cardiac graft was transplanted into the cervical location in the recipient GEP expressions in the cardiac graft were maximally detected at 3 days after gene transfer and persisted for 3 weeks.

本研究采用金颗粒包裹的DNA作为抗休克载体，将GFP基因转染COS-7细胞，观察其对移植物冠状动脉粥样硬化的抑制作用。在接下来的研究中，我们切取供体心脏，采用反休克法将GFP基因转入心脏移植物中。然后将移植心脏移植到受体颈部，移植心脏中的GEP表达在基因转移后3d达到最大，并持续3周。

项目成果

Y.Umeda, H.Iwata, S.Yoshikawa, Y.Matsuno, T.Marui, Y.Iida, H.Takagi, Y.Mori, J.Miyazaki, A.Kosugi, H.Hirose: "Gene Gun-Mediated CTLA4Ig-Gene Transfer for Modification of Allogeneic Cardiac"Transplantation Proceedings. 34. 2622-2623 (2002)

Y.Umeda、H.Iwata、S.Yoshikawa、Y.Matsuno、T.Marui、Y.Iida、H.Takagi、Y.Mori、J.Miyazaki、A.Kosugi、H.Hirose：“基因枪介导的 CTLA4Ig

Y.Matsuno, H.Iwata, S.Yoshikawa, Y.Umeda, Y.Iida, H.Takagi, Y.Mori.J.Miyazaki, A.Kosugi, H.Hirose: "Suppression of Graft Coronary Arteriosclerosis by Gene Gun-Mediated CTLA4-Ig Gene Transfer"Transplantation Proceedings. 34. 2619-2621 (2002)

Y.Matsuno、H.Iwata、S.Yoshikawa、Y.Umeda、Y.Iida、H.Takagi、Y.Mori.J.Miyazaki、A.Kosugi、H.Hirose：“基因枪抑制移植冠状动脉硬化-

Y.Matsuno, H.Iwata, S.Yoshikawa, Y.Umeda, Y.Iida, H.Takagi, Y.Mori, J.Miyazaki, A.Kosugi, H.Hirose: "Suppression of Graft Coronary Arteriosclerosis by Gene Gun-Mediated CTLA4-Ig Gene Transfer"Transplantation Proceedings. 34. 2619-2621 (2002)

Y.Matsuno、H.Iwata、S.Yoshikawa、Y.Umeda、Y.Iida、H.Takagi、Y.Mori、J.Miyazaki、A.Kosugi、H.Hirose：“基因枪抑制移植冠状动脉硬化-

Y.Umeda, H.Hirose, S.Yoshikawa, Y.Matsuno, Y.Fukumoto, T.Nitta, Y.Iida, H.Takagi, Y.Mori, D.Liddicoat, J.Miyazaki, A.Kosugi, H.Hirose: "Nonviral Gene Gun-Mediated CTLA4-Ig Gene Transfer for Modification of Donor Organs"Transplantation Proceedings. 33. 243

Y.Umeda、H.Hirose、S.Yoshikawa、Y.Matsuno、Y.Fukumoto、T.Nitta、Y.Iida、H.Takagi、Y.Mori、D.Liddicoat、J.Miyazaki、A.Kosugi、H.

Y.Umeda, H.Hirose, S.Yoshikawa, Y.Matsuno, Y.Fukumoto, T.Nitta, Y.Iida, H.Takagi, Y.Mori, D.Liddicoat, J.Miyazaki, A.Kosugi, H.Iwata: "Nonviral Gene Gun-Mediated CTLA4-Ig Gene Transfer for Modification of Donor Organs"Transplantation Proceedings. 33. 243-

Y.Umeda、H.Hirose、S.Yoshikawa、Y.Matsuno、Y.Fukumoto、T.Nitta、Y.Iida、H.Takagi、Y.Mori、D.Liddicoat、J.Miyazaki、A.Kosugi、H.