Gene therapy for graft coronary arteriosclerosis targeted at smooth muscle progenitor cells derived from the recipients' bone marrow
Gene therapy for graft coronary arteriosclerosis targeted at smooth muscle progenitor cells derived from the recipients' bone marrow
批准号:
14370407
负责人:
MORI Yoshio
金额:
$8.77万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (B)
财政年份:
2002
资助国家:
日本
项目状态:
已结题
起止时间:
2002 至 2003
中文摘要
本研究采用金颗粒包裹的DNA作为抗休克载体,将GFP基因转染COS-7细胞,观察其对移植物冠状动脉粥样硬化的抑制作用。在接下来的研究中,我们切取供体心脏,采用反休克法将GFP基因转入心脏移植物中。然后将移植心脏移植到受体颈部,移植心脏中的GEP表达在基因转移后3d达到最大,并持续3周。
英文摘要
The purpose in this study is that whether ox not suppress graft coronary arteriosclerosis using by counter shock method of the DNA coated around the gold particle.DNA vector of the GFP gene was transferred to COS-7 cells in vitro using counter shock method. Then the expression of the GFP gene was confirmed by fluorescent microscopy.In the next study, the donor heart was excised and the GFP gene was transferred into the cardiac graft using counter shock method. Then the cardiac graft was transplanted into the cervical location in the recipient GEP expressions in the cardiac graft were maximally detected at 3 days after gene transfer and persisted for 3 weeks.
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Y.Umeda, H.Iwata, S.Yoshikawa, Y.Matsuno, T.Marui, Y.Iida, H.Takagi, Y.Mori, J.Miyazaki, A.Kosugi, H.Hirose: "Gene Gun-Mediated CTLA4Ig-Gene Transfer for Modification of Allogeneic Cardiac"Transplantation Proceedings. 34. 2622-2623 (2002)
Y.Umeda、H.Iwata、S.Yoshikawa、Y.Matsuno、T.Marui、Y.Iida、H.Takagi、Y.Mori、J.Miyazaki、A.Kosugi、H.Hirose:“基因枪介导的 CTLA4Ig
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Y.Matsuno, H.Iwata, S.Yoshikawa, Y.Umeda, Y.Iida, H.Takagi, Y.Mori.J.Miyazaki, A.Kosugi, H.Hirose: "Suppression of Graft Coronary Arteriosclerosis by Gene Gun-Mediated CTLA4-Ig Gene Transfer"Transplantation Proceedings. 34. 2619-2621 (2002)
Y.Matsuno、H.Iwata、S.Yoshikawa、Y.Umeda、Y.Iida、H.Takagi、Y.Mori.J.Miyazaki、A.Kosugi、H.Hirose:“基因枪抑制移植冠状动脉硬化-
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Y.Matsuno, H.Iwata, S.Yoshikawa, Y.Umeda, Y.Iida, H.Takagi, Y.Mori, J.Miyazaki, A.Kosugi, H.Hirose: "Suppression of Graft Coronary Arteriosclerosis by Gene Gun-Mediated CTLA4-Ig Gene Transfer"Transplantation Proceedings. 34. 2619-2621 (2002)
Y.Matsuno、H.Iwata、S.Yoshikawa、Y.Umeda、Y.Iida、H.Takagi、Y.Mori、J.Miyazaki、A.Kosugi、H.Hirose:“基因枪抑制移植冠状动脉硬化-
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Y.Umeda, H.Hirose, S.Yoshikawa, Y.Matsuno, Y.Fukumoto, T.Nitta, Y.Iida, H.Takagi, Y.Mori, D.Liddicoat, J.Miyazaki, A.Kosugi, H.Hirose: "Nonviral Gene Gun-Mediated CTLA4-Ig Gene Transfer for Modification of Donor Organs"Transplantation Proceedings. 33. 243
Y.Umeda、H.Hirose、S.Yoshikawa、Y.Matsuno、Y.Fukumoto、T.Nitta、Y.Iida、H.Takagi、Y.Mori、D.Liddicoat、J.Miyazaki、A.Kosugi、H.
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Y.Umeda, H.Hirose, S.Yoshikawa, Y.Matsuno, Y.Fukumoto, T.Nitta, Y.Iida, H.Takagi, Y.Mori, D.Liddicoat, J.Miyazaki, A.Kosugi, H.Iwata: "Nonviral Gene Gun-Mediated CTLA4-Ig Gene Transfer for Modification of Donor Organs"Transplantation Proceedings. 33. 243-
Y.Umeda、H.Hirose、S.Yoshikawa、Y.Matsuno、Y.Fukumoto、T.Nitta、Y.Iida、H.Takagi、Y.Mori、D.Liddicoat、J.Miyazaki、A.Kosugi、H.
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共 6 条
Study on postwar Okinawa and philosophy of history in the global era
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批准号:24520785
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项目类别:Grant-in-Aid for Scientific Research (C)
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资助金额:$3.33万
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财政年份:2012
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负责人:MORI Yoshio
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依托单位:
Study on mechanisms for formation of genome replication vesicles produced by plus-strand RNA viruses
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批准号:23689028
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项目类别:Grant-in-Aid for Young Scientists (A)
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资助金额:$17.56万
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财政年份:2011
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负责人:MORI Yoshio
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依托单位:
A Study on the Social Movements and Thought over the Administrative Reversion of Okinawa
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批准号:20520603
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项目类别:Grant-in-Aid for Scientific Research (C)
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资助金额:$2.91万
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财政年份:2008
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负责人:MORI Yoshio
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依托单位:
Study on enhancement of Japanese encephalitis virus replication by the core protein
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批准号:19780226
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项目类别:Grant-in-Aid for Young Scientists (B)
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资助金额:$2.48万
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财政年份:2007
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负责人:MORI Yoshio
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依托单位:
Gene gun mediated CTLA4-Ig gene transfer for immunological tolerance induction
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批准号:11470269
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项目类别:Grant-in-Aid for Scientific Research (B).
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资助金额:$9.22万
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财政年份:1999
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负责人:MORI Yoshio
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依托单位:
DIELECTRIC SPECTROSCOPY FOR MONITOR OF INTRA OPERATIVE MYOCARDIUM
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批准号:08671520
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项目类别:Grant-in-Aid for Scientific Research (C)
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资助金额:$1.34万
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财政年份:1996
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负责人:MORI Yoshio
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依托单位:
Study of cytotoxic Killer T cell against target liver cells and effect of prostaglandins - inhibitory effect of prostaglandin El on T cell-mediated cytotoxicity.
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批准号:62570308
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项目类别:Grant-in-Aid for General Scientific Research (C)
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资助金额:$1.47万
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财政年份:1987
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负责人:MORI Yoshio
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依托单位: