Gene therapy for graft coronary arteriosclerosis targeted at smooth muscle progenitor cells derived from the recipients' bone marrow

本研究采用金颗粒包裹的DNA作为抗休克载体，将GFP基因转染COS-7细胞，观察其对移植物冠状动脉粥样硬化的抑制作用。在接下来的研究中，我们切取供体心脏，采用反休克法将GFP基因转入心脏移植物中。然后将移植心脏移植到受体颈部，移植心脏中的GEP表达在基因转移后3d达到最大，并持续3周。

The purpose in this study is that whether ox not suppress graft coronary arteriosclerosis using by counter shock method of the DNA coated around the gold particle.DNA vector of the GFP gene was transferred to COS-7 cells in vitro using counter shock method. Then the expression of the GFP gene was confirmed by fluorescent microscopy.In the next study, the donor heart was excised and the GFP gene was transferred into the cardiac graft using counter shock method. Then the cardiac graft was transplanted into the cervical location in the recipient GEP expressions in the cardiac graft were maximally detected at 3 days after gene transfer and persisted for 3 weeks.