Study on mechanisms for formation of genome replication vesicles produced by plus-strand RNA viruses

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The common hallmark of plus-strand RNA viruses is to synthesize their genomes at various organelle membranes. In this study, we attempted to isolate viral and host factors for genome synthesis of plus-strand RNA viruses and common mechanisms underlying genome replication. In order to quantify, the subgenomic replicon of rubella virus, a member of plus-strand RNA viruses, was established in this study, and using this system viral and host factors for promoting viral propagation were identified. Furthermore, this is the first study to indicate that rubella virus undergoes the genome synthesis at the plasma membrane. The results in this study provide the information for understanding of mechanisms underlying genome replication of plus-strand RNA viruses.