Study of cytotoxic Killer T cell against target liver cells and effect of prostaglandins - inhibitory effect of prostaglandin El on T cell-mediated cytotoxicity.

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The effects of prostaglandin El on cell-mediated cytotoxicity against hepatocytes were investigated using an in vitro cytotoxic assay system. Isolated liver cells from normal C57BL/6 mice were used as the target cells, and effector cells were obtained from spleen cells of C57BL/6 mice in which experimental hepatitis had been induced by immunization with syngeneic liver antigens. In this assay system, spleen T cells adherent to nylon wool demonstrated a high cytotoxicity against liver cells. The cytotoxicity was markedly reduced by prostaglandin El at concentrations 10^7 M. Maximum suppressive activity was obtained when prostaglandin El was continuously present during the assay period. By contrast, indomethacin, a specific inhibitor of prostaglandin sythesis, enhanced the cytotoxic activity of effector cells. These data seem to indicate that exogenously added prostaglandin El has an inhibitory effect on cell-mediated cytotoxicity of effector spleen cells against target hepatocytes.