Study of cytotoxic Killer T cell against target liver cells and effect of prostaglandins - inhibitory effect of prostaglandin El on T cell-mediated cytotoxicity.

针对靶肝细胞的细胞毒性杀伤T细胞和前列腺素的作用的研究-前列腺素E1对T细胞介导的细胞毒性的抑制作用。

• 批准号: 62570308
• 负责人: MORI Yoshio
• 金额: $ 1.47万
• 依托单位: Chiba University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1987
• 资助国家: 日本
• 起止时间: 1987 至 1988
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-62570308/
• 关键词: Experimental hepatitis; Cytotoxic killer T cell; Prostaglandin El; インドメサシン; 肝特異的キラーT細胞; 細胞障害性キラーT細胞(CTL); 肝障害; プロスタグランディン; PGE_1

The effects of prostaglandin El on cell-mediated cytotoxicity against hepatocytes were investigated using an in vitro cytotoxic assay system. Isolated liver cells from normal C57BL/6 mice were used as the target cells, and effector cells were obtained from spleen cells of C57BL/6 mice in which experimental hepatitis had been induced by immunization with syngeneic liver antigens. In this assay system, spleen T cells adherent to nylon wool demonstrated a high cytotoxicity against liver cells. The cytotoxicity was markedly reduced by prostaglandin El at concentrations 10^7 M. Maximum suppressive activity was obtained when prostaglandin El was continuously present during the assay period. By contrast, indomethacin, a specific inhibitor of prostaglandin sythesis, enhanced the cytotoxic activity of effector cells. These data seem to indicate that exogenously added prostaglandin El has an inhibitory effect on cell-mediated cytotoxicity of effector spleen cells against target hepatocytes.

采用体外细胞毒实验系统研究前列腺素EL对肝细胞介导的细胞毒作用的影响。以正常C57BL/6小鼠分离的肝细胞为靶细胞，以同基因肝抗原免疫诱导实验性肝炎的C57BL/6小鼠脾细胞为靶细胞。在该检测体系中，附着在尼龙毛上的脾T细胞对肝细胞具有较高的细胞毒作用。前列腺素EL在107M浓度下可显著降低细胞毒作用，当前列腺素EL在实验期间持续存在时，抑制活性达到最大。相比之下，前列腺素合成的特异性抑制剂吲哚美辛则增强了效应细胞的细胞毒活性。提示外源性前列腺素EL可抑制效应脾细胞对靶肝细胞的细胞毒作用。

项目成果

Mori Y. Mori T. Yoshida H. et al: Clin. Exp. Immunol. 57. 85-92 (1984)

Mori Y. Mori T. Yoshida H. 等人：临床。

Mori Y. Mori T. Ueda S. et al: Clin. Exp. Immunol.61. 577-584 (1985)

Mori Y. Mori T. Ueda S. 等人：临床。

M.Ogawa,;Y.Mori,;T.Mori,et al.: Cli.Exp.Immunol.73. 276-282 (1988)

M.Okawa,;Y.Mori,;T.Mori,et al.:Cli.Exp.Immunol.73。

森義雄: "日本臨床1988年増刊号「肝胆膵」-新しい診断、治療体系-" 日本臨床社, 215-222 (1988)

Yoshio Mori：“Nippon Clinical 1988 特刊‘肝胆胰’-新的诊断和治疗系统 -”Nippon Clinical Publishing，215-222（1988）

森義雄,小川真,森照男 他: 肝臓. 28. 283-290 (1987)

Yoshio Mori、Makoto Okawa、Teruo Mori 等：肝脏。28. 283-290 (1987)