Analysis of the function of TM4SF complex and lung cancer gene therapy based on using this complex.

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Establishment of cell lines in each stage plays a most important role to clarify abnormality of the TM4SF complex. Previously, we reported mixing 50% matrigel in this collagen is the most efficient for establishment of the cell lines. Adding 10% of patients' serum in the above mixture made it easier to establish the cell line. Using this method, three kinds of AAH were established. There were no abnormality in the expression of MRP-1/CD9、KAI1/CD82 in these cell lines. And also PETA3/CD15I expression appeared decreasing. Next, 10 cell lines were established in Stage I lung cancer, 9 cell lines were established in Stage II lung cancer. We tried to establish 13 cell lines in Stage III lung cancer that did chemotherapy before the operation, however only 4 cell lines were established because most of the parts were necrotic region. Six kinds of cell lines in StageIV were established from the specimens of metastasis region. Then, the complex formation of MRP-1/CD9、KAI1/CD82 and PETA3/CD 151 were analyzed. In the Stage I, MRP-1/CD9 and KAI1/CD82 expression were found in two cell lines except one, but PETA3/CDI5I appeared weak expression. In the Stage II, KAI1/CD82 was decreasing in 5 cases and MRP-1/CD9 was decreasing in I. case, however PETA3/CD151 was increasing in 2 cases. In the StageIII, only KAII/CD82was found decreasing in 4 cases. Then only PETA3/CD151 was appeared in the last 1case. In the StageIV、 expression of PETA3/CD151 was positive in almost all metastatic cell lines. From these experiments, MRP-1/CD9 and KAI1/CD82 play an important role as metastasis suppressor genes. On the other hand, PETA3/CD151 plays a metastasis promotion gene. Hence, the balance and unbalance of these expressions have something to do with the process of metastasis.