权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Analysis of the function of TM4SF complex and lung cancer gene therapy based on using this complex.

TM4SF复合物的功能分析及基于该复合物的肺癌基因治疗。

基本信息

批准号：
14370421
负责人：
MIYAKE Masayuki
金额：
$ 9.02万
依托单位：
The Tazuke Kofukai
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2003
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14370421/
关键词：
TM4SF AAH MRP-1 CD9 KAI1 CD82 PETA3 CD151 metaplasia dysplasia

项目摘要

Establishment of cell lines in each stage plays a most important role to clarify abnormality of the TM4SF complex. Previously, we reported mixing 50% matrigel in this collagen is the most efficient for establishment of the cell lines. Adding 10% of patients' serum in the above mixture made it easier to establish the cell line. Using this method, three kinds of AAH were established. There were no abnormality in the expression of MRP-1/CD9、KAI1/CD82 in these cell lines. And also PETA3/CD15I expression appeared decreasing. Next, 10 cell lines were established in Stage I lung cancer, 9 cell lines were established in Stage II lung cancer. We tried to establish 13 cell lines in Stage III lung cancer that did chemotherapy before the operation, however only 4 cell lines were established because most of the parts were necrotic region. Six kinds of cell lines in StageIV were established from the specimens of metastasis region. Then, the complex formation of MRP-1/CD9、KAI1/CD82 and PETA3/CD 151 were analyzed. In the Stage I, MRP-1/CD9 and KAI1/CD82 expression were found in two cell lines except one, but PETA3/CDI5I appeared weak expression. In the Stage II, KAI1/CD82 was decreasing in 5 cases and MRP-1/CD9 was decreasing in I. case, however PETA3/CD151 was increasing in 2 cases. In the StageIII, only KAII/CD82was found decreasing in 4 cases. Then only PETA3/CD151 was appeared in the last 1case. In the StageIV、 expression of PETA3/CD151 was positive in almost all metastatic cell lines. From these experiments, MRP-1/CD9 and KAI1/CD82 play an important role as metastasis suppressor genes. On the other hand, PETA3/CD151 plays a metastasis promotion gene. Hence, the balance and unbalance of these expressions have something to do with the process of metastasis.

每个阶段细胞系的建立对于澄清TM4SF复合物的异常起着最重要的作用。之前，我们报道过在这种胶原蛋白中混合 50% 基质胶对于细胞系的建立最有效。在上述混合物中添加10%的患者血清可以更容易地建立细胞系。使用该方法，建立了三种AAH。各细胞系中MRP-1/CD9、KAI1/CD82表达均未见异常。 PETA3/CD15I 表达也出现下降。接下来，在I期肺癌中建立了10个细胞系，在II期肺癌中建立了9个细胞系。我们试图在术前化疗的Ⅲ期肺癌中建立13个细胞系，但由于大部分部位都是坏死区域，只建立了4个细胞系。从转移区标本中建立了六种第四期细胞系。然后分析了MRP-1/CD9、KAI1/CD82和PETA3/CD 151复合物的形成。在第一阶段，除1个细胞系外，2个细胞系均表达MRP-1/CD9和KAI1/CD82，但PETA3/CDI5I表达较弱。在II期，5例KAI1/CD82下降，I例MRP-1/CD9下降，但2例PETA3/CD151上升。 III期仅4例KAII/CD82下降。然后最后1例中只出现了PETA3/CD151。在第四阶段，PETA3/CD151在几乎所有转移细胞系中均呈阳性表达。从这些实验来看，MRP-1/CD9和KAI1/CD82作为转移抑制基因发挥着重要作用。另一方面，PETA3/CD151起着转移促进基因的作用。因此，这些表达的平衡和不平衡与转移过程有关。