The devebpment of artificial pituitary grand.

人工垂体的发育。

• 批准号: 14370435
• 负责人: KURISU Kaoru
• 金额: $ 8.06万
• 依托单位: HIROSHIMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14370435/
• 关键词: pituitary grand; polymer gel; pituitary hormone; anterior lobe; hypothalamic hormone; fractionational molecular weight; HEA gel; growth hormone

The transplantation of encapsulated growth hormone-secreting cells represents one potential means of treating GH deficiency. The present study investigated the ability of encapsulated GH3 cells, which are derived from rat somatotropin secreting pituitary rumor, to supply GH in the long term and to effect functional improvement in the rat models. Following polymer encapsulation, GH3 cells were transplanted into the rats. A suspension of 6x10^6 cells in agarose gel was injected into a Millipore chamber consisting of a Millipore filter ring and two Isopore disc Polycarbonate hydrophilic 0.4 μm Durapore filters, which were attached to the ring with Aron Alpha. This chamber was implanted into Wister rat by making subcuatenous tunnel. The secretion of GH from the encapsulated cells, and functional changes in the host animals were examined 3 months after transplantation. Analysis of transplanted rat showed that serum GH level were elevated 3 month after tranaplantation. However, there is no statistical difference in tail length and the weight between microcapsule transplanted rats and untreated rats. Analysis of retrieved capsules revealed that the GH3 cells survived and continued to release GH even 3 months after transplantation. These results further support the potential use of this approach for the treatment of GH deficiency.On the other hand, we constructed original microcapsule based on 2-hydroxy-ethyl acrylate(HEA) polymer gel. We could get size fractionation between IgG and pituitary hormone size of dextran by this HEA based polymer.In clinical research, we found that apparent diffusion coefficient of craniopharyngiomas was higher than that of pituitary adenomas. We found a positive relationship between VEGF expression and hemorrhage in pituitary adenoma. And we reported that assessment of the postoperative endocrinologic status should be delayed at least one month after surgery in acromegalic patients.

微囊化生长激素分泌细胞的移植是治疗GH缺乏症的一种潜在手段。本研究探讨了囊化的GH 3细胞的能力，这是来自大鼠生长激素分泌垂体瘤，提供生长激素在长期和影响功能改善的大鼠模型。聚合物包封后，将GH 3细胞移植到大鼠体内。将琼脂糖凝胶中的6 × 10^6个细胞悬液注射到由Millipore过滤环和两个Isopore圆盘聚碳酸酯亲水性0.4 μm Durapore过滤器组成的Millipore室中，过滤器用Aron Alpha连接到环上。通过制作皮下隧道的方法将该小室植入Wister大鼠体内。移植后3个月，检测微囊化细胞分泌GH的情况和宿主动物的功能变化。对移植大鼠的分析表明，移植后3个月血清GH水平升高。而微囊移植组大鼠的尾长和体重与未移植组大鼠相比无统计学差异。对回收的胶囊的分析显示，GH 3细胞存活，甚至在移植后3个月仍继续释放GH。这些结果进一步支持了这种方法在治疗GH缺乏症方面的潜在应用。另一方面，我们构建了基于2-羟基乙基丙烯酸酯（HEA）聚合物凝胶的原始微胶囊。用这种HEA基聚合物可以得到IgG和垂体激素分子大小的右旋糖酐分子之间的尺寸分级。临床研究中发现颅咽管瘤的表观扩散系数高于垂体腺瘤。我们发现VEGF的表达与垂体腺瘤出血呈正相关。我们还报道了肢端肥大症患者术后内分泌状态的评估应延迟至少一个月。

项目成果

Relationship between intratumoral hemorrhage and overexpression of vascular endothelial growth factor(VEGF) in pituitary adenoma.

垂体腺瘤瘤内出血与血管内皮生长因子（VEGF）过表达的关系

• 发表时间: 2004
• 期刊: Hiroshima J Med Sci 53
• 作者: Arita K

プロラクチノーマの外科治療-その適応と禁忌-

泌乳素瘤的手术治疗 - 其适应症和禁忌症 -

• 发表时间: 2002
• 期刊: 日本内分泌学会雑誌 78
• 作者: Youichi Saitoh;富永 篤;有田 和徳;Kato Amami;Eguchi Yutaka;Arita K;Saitoh Y;富永 篤;Saitoh Y;Tomishima T;有田 和徳
• 通讯作者: 有田 和徳

Infundibuloneurohypophysitis presenting a large sellar-juxtaselllar mass.

漏斗神经垂体炎表现为较大的鞍旁肿块。

• 发表时间: 2004
• 期刊: Surgical Neurology 61
• 作者: Kanou Y

Infundibuloneurohypophysitis presenting a large sellar-juxtasellar mass

漏斗神经垂体炎表现为较大的鞍旁肿块

• 发表时间: 2004
• 期刊: Surgical Neurology 61
• 作者: Kanou Y

Detection and differentiation of lactate and lipids by single-voxel proton MR spectroscopy

• DOI: 10.1007/s10143-005-0398-1
• 发表时间: 2005-08
• 期刊: Neurosurgical Review
• 影响因子: 2.8
• 作者: F. Yamasaki;J. Takaba;M. Ohtaki;N. Abe;Y. Kajiwara;T. Saito;H. Yoshioka;S. Hama;T. Akimitsu;K. Sugiyama;K. Arita;K. Kurisu