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Machanism of Progresses renal disease studied by a magnifying-endoscopy

通过放大内窥镜研究肾脏疾病的进展机制

基本信息

批准号：
14370509
负责人：
ONO Yoshinari
金额：
$ 7.49万
依托单位：
Nagoya University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2004
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14370509/
关键词：
peritubular capillary microcirculation a magnifying-endoscopy enabling cadaveric transplants living donor transplants warm and cold ischemia acute tubular necrosis direct imaging system Human kidney transplantation 慢性移植腎腎症 chronic reje

项目摘要

We developed a direct imaging system of renal microcirculation by a magnifying-endoscopy enabling visualization of the movement of erythrocyte in glumerular and cortical peritubular capillary(CPC). We investigated the microcirculation of CPC in the early phase of both living and cadaveric donor transplant kidneys. Erythrocyte velocity in CPC were monitored and measured in twenty renal transplants at 20,60,90 and 120 minutes after reperfusion. The kidney grafts came from 11 living donors and 9 non-heart beating cadaveric donors. In living donor transplants, erythrocyte velocity in CPC at 20 minutes after revascularization declined to one third of base line value just before nephrectomy, and recovered to the pre-nephrectomy value 120minutes after reperfusion. In contrast, it continued to be disturbed for 90 minutes in cadaveric donor transplants. Erythrocyte velocity in CPC more significantly deteriorated in cadaveric transplants than in those of living transplants at 20 through 60 minutes after the revascularization.In living donor transplants, erythrocyte velocity did not correlate with donor age, both warm and cold ischemic time, time to the initial urination and best creatine clearance. In the cadaveric transplants ischemic time, both WIT and CIT, did not correlate with the erythrocyte velocity. However, donor age, duration of ATN and best creatine clearance after transplantation significantly correlated with the erythrocyte velocity.The measurement of erythrocyte velocity in CPC is a reliable method for predicting the recovery of renal function and reserved renal function of kidney allografts undergoing prolonged ischemia.

我们研制了一种肾微循环直接成像系统，通过放大内窥镜可以观察到红细胞在肾小球和皮质管周毛细血管（CPC）中的运动。我们研究了活体和尸体供肾移植早期CPC的微循环。对20例肾移植术后再灌注20、60、90和120分钟的红细胞速度进行了监测。供肾来自11例活体供肾和9例无心跳尸体供肾。在活体供者移植中，CPC中的红细胞速度在血运重建后20分钟下降到肾切除前的基线值的1/3，并在再灌注后120分钟恢复到肾切除前的值。相比之下，在尸体供体移植中，它继续被干扰90分钟。红细胞在CPC的速度更显着恶化，尸体移植比那些在活体移植在20至60分钟后，在revascularization.In活体供体transplants，红细胞速度没有相关性与供体年龄，无论是热缺血和冷缺血时间，时间到首次排尿和最佳肌酸清除。在尸体移植的缺血时间，无论是WIT和CIT，与红细胞的速度没有相关性。红细胞速率与供者年龄、ATN持续时间及移植后最佳肌酸清除率显著相关，CPC红细胞速率测定是预测长期缺血移植肾功能恢复及保留肾功能的可靠方法。