Effects of Internal Effluents of Dentin Adhesive Materials on Neutrophil Proliferation and Differentiation

牙本质粘接材料内部流出物对中性粒细胞增殖和分化的影响

• 批准号: 14370613
• 负责人: ARAKI Kouji
• 金额: $ 6.27万
• 依托单位: Tokyo Medical and Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14370613/
• 关键词: adhesive resin; effluents; cytotoxicity; cellular proliferation; pulp derived cell; DNA amount; mRNA; HL-60細胞; 4-META; 培養細胞; 増殖機能; レジンプレート

Dentin adhesive resins not only have been useful for restoration in caries treatments, but have expanded the range of possibilities for the direct pulp capping technique. Therefore, its effluents might have direct effects on pulp tissues, on periodontal tissues, or on periapical tissues without through dentin. It is said that effluents of dentin adhesive materials have almost no cytotoxicity in cell viability assays. But they have possibilities to cause various molecular changes on individual cells. In this study, focusing on molecular biology, we evaluated effects of internal effluents of 4-META/MMA-TBB on cellular proliferation and differentiation in established cell line to demonstrate the following things.1.It was suggested that 4-META/MMA-TBB had small effects on cellular proliferation and on ALP activity of clonal rat dental pulp cells cultured on 4-META/MMA-TBB plates.2.4-META/MMA-TBB was thought to have small effects on mRNA expression of ALP, Coll(I), OC, and BMP2 of the rat dental pulp cells cultured on 4-META/MMA-TBB plates.3.4-META/MMA-TBB monomer (concentration of more than 10-1%) was thought to have toxic effects inhibited proliferation of clonal rat dental pulp cells.4.4-META/MMA-TBB monomer (concentration of more than 10-1%) was thought to have toxic effects inhibited proliferation of clonal human lymphocyte-like cells.

牙本质粘合树脂不仅可用于龋齿治疗中的修复，而且扩大了直接盖髓技术的可能性范围。因此，其流出物可能直接作用于牙髓组织、牙周组织或根尖周组织，而不穿透牙本质。据报道，牙本质粘附材料的流出物在细胞活力测定中几乎没有细胞毒性。但它们有可能在单个细胞上引起各种分子变化。在这项研究中，专注于分子生物学，本研究通过观察4-Meta/MMA-TBB内分泌物对体外培养的大鼠牙髓细胞增殖和分化的影响，发现4-Meta/MMA-TBB内分泌物对体外培养的大鼠牙髓细胞增殖和ALP活性的影响较小，而对体外培养的大鼠牙髓细胞增殖和ALP活性的影响较小。认为TBB对ALP、科尔（I）、OC、3.4-Meta/MMA-TBB单体4.4-Meta/MMA-TBB单体（浓度超过10-1%）被认为具有抑制克隆大鼠牙髓细胞增殖的毒性作用。（浓度超过10-1%）被认为具有抑制克隆人淋巴细胞样细胞增殖的毒性作用。

项目成果

Effects of exposure to 4-META/MMA-TBB on Pulp Cell Differentiation

暴露于 4-META/MMA-TBB 对牙髓细胞分化的影响

• 发表时间: 2005
• 期刊: Journal of Dental Research 84巻特別号(発表予定)
• 作者: Sasano T;Onodera D;Hashimoto K;Iikubo M;Satoh-Kuriwada S;Shoji N;Miyahara T;IMAIZUMI NAKAKO
• 通讯作者: IMAIZUMI NAKAKO

Effects of chloramines and sodium hypochlorite on carious dentine

氯胺和次氯酸钠对龋齿牙本质的影响

• 发表时间: 2003
• 期刊: Journal of Medical and Dental Sciences 50
• 作者: Tonami Ken-ichi

今泉名香子: "Effects of an Adhesive Resin on Pulp Cell Viability"Journal of Dental Research. 83(発表予定). (2004)

Nako Imaizumi：“粘合树脂对牙髓细胞活力的影响”牙科研究杂志 83（待出版）。