Study on the differentiation from human dental pulp to odontoblast using organ culture

器官培养法研究人牙髓向成牙本质细胞的分化

• 批准号: 07457452
• 负责人: ARAKI Kouji
• 金额: $ 4.16万
• 依托单位: Tokyo Medical and Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07457452/
• 关键词: human dental pulp; organ culture; odontoblast; differenciation; cytokine; mRNA; LPS; detal cement; ヒト歯髄; 分子生物学

From1995 to three years , we have studied about several causes of the differentiation from a human pulp cell to odontoblast biochemically and molecular biologically. As a result, several important facts were obtained.1. When cultured fibroblastic cells derived from human pulp were affected indirectly by the extract components of several dental cements, certain cement was very low cytotoxic. This extract solution was thought as usefulness as a differentiation induction substance in vitro experiment.2. The increase of Alkaline phosphatase activity in the pulp organ culture was considered as an index of the differentiation to odontoblast.Even if TGF-beta 1 or BMP is given alone to a human pulp organ culture, obvious evidence of the differentiation of an odontoblast or a hard tissue formation were not obtained.When some cytokine mRNA expression in the inflammatory and normal extirpated human pulp was searched by using RT-PCR,the TGF-beta 1 mRNA expression was always identified from various clinical condition of dental pulp.

从1995年到3年，我们对人牙髓细胞向成牙本质细胞分化的几个原因进行了生化和分子生物学研究。结果，得出了几个重要的事实。当培养的人牙髓成纤维细胞受到几种牙科粘固剂提取物成分的间接影响时，某些粘固剂的细胞毒性很低。结论：1.该提取液在体外实验中具有诱导分化的作用。牙髓器官培养中碱性磷酸酶活性的升高被认为是向成牙本质细胞分化的一个指标。即使单独给予转化生长因子-β1或骨形态发生蛋白，也没有明显的成牙本质细胞分化或硬组织形成的证据。当用RT-PCR检测炎症和正常摘除的人牙髓中某些细胞因子的mRNA表达时，在不同的临床条件下都能检测到转化生长因子-β1mRNA的表达。

项目成果

K.Araki: "Effects of Dental Cements on Pruiiferation of Rat Pulp Cells" Journal of Dental Research. (in press).

K.Araki：“牙科水泥对大鼠牙髓细胞增殖的影响”牙科研究杂志。

R.WADACHI: "The effects of calcium hydroxide on the Dissolution of soft tissue on the root canal" J of Endodontics. (in press).

R.WADACHI：“氢氧化钙对根管内软组织溶解的影响”《牙髓学杂志》。

伊藤 律子: "Insert Chamberを用いた歯科用セメントの細胞毒性評価" 日本歯科保存学雑誌. 38・4. 1048-1056 (1995)

伊藤律子：“使用插入室评估牙科水泥的细胞毒性”日本保守牙科杂志 38・4（1995）。

澤田 則宏: "歯科材料の副作用と安全性-歯内療法材料" 学建書院, 173 (1997)

泽田典宏：“牙科材料的副作用和安全性——牙髓治疗材料”学研书院，173（1997）

R.ITOH: "Cytotoxic evaluation of dental cements using insert chamber method" Jap J Conserv Dent. 38(4). 1048-1056 (1995)

R.ITOH：“使用插入室法对牙科水泥进行细胞毒性评估”Jap J Conserv Dent。