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investigation of bFGF potential for egenerative therapy of dentin-pulp complex

bFGF 牙本质-牙髓复合体再生治疗潜力的研究

基本信息

批准号：
14370617
负责人：
SHIMABUKURO Yoshio
金额：
$ 8.64万
依托单位：
Osaka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2004
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14370617/
关键词：
dentin-pulp complex pulp cells periodontal ligament cells hyaluronan regenerative therapy heparan sulfate cell migration basic fibroblast growth factor 塩基性繊維芽細胞増殖因子創傷治癒ヒアルロン酸合成酵素シンデカンヒト歯髄細胞 FGF-2 覆髄剤修復象牙質石灰化 ALPase活

项目摘要

We examined the effects of FGF-2 on the production of heparan sulfate and hyaluronan by and expression of hyaluronan synthase in human dental pulp cells and periodontal ligament cells. BFGF increased the production of heparan sulfate by human periodontal ligament cells by using enzyme linked immunosorbent assay. Flow cytometric analysis revealed that syndecan-1 expression did not altered but syndecan-2 expression was decreased when human periodontal ligament cells were stimulated with bFGF. In addition, bFGF stimulated the production of hyalurona a by both human dental pulp cells and periodontal ligament cells and the expression of hyaluronan synthase 1 and 2. mRNA. bFGF enhanced cell migration of human dental pulp cells and the bFGF-induced cell migration was inhibited anti-bFGF neutralizing antibody, which suggest that the migratory action is dependent on bFGF. Moreover, the bFGF-increased cell migration of human dental pulp cells was still observed in spite of suppression of cell proliferation with mitomycin C treatment of the cells. These results suggest that the migration of human dental pulp cells induced with bFGF was independent of cell proliferation. Taken together, bFGF may modulate the wound healing with regulating production of extracellular matrix and cell migration. In vivo application of bFGF on the pulp exposure site of the forth anterior teeth of beagle dogs led to acceleration of bard tissue formation compared with control. Therefore, bFGF appears to be useful reagent as regenerative therapy of dentin-pulp complex.

我们通过人牙髓细胞和牙周韧带细胞透明质酸合成酶的表达，观察了成纤维细胞生长因子-2对人牙髓细胞和牙周韧带细胞产生硫酸肝素和透明质酸的影响。采用酶联免疫吸附试验检测碱性成纤维细胞生长因子促进人牙周韧带细胞产生硫酸乙酰肝素。流式细胞仪分析显示，碱性成纤维细胞生长因子刺激人牙周膜细胞后，Syndecan-1的表达没有改变，而Syndecan-2的表达降低。此外，碱性成纤维细胞生长因子刺激人牙髓细胞和牙周韧带细胞产生透明质酸a，并促进透明质酸合成酶1和2的表达。碱性成纤维细胞生长因子促进人牙髓细胞的迁移，其诱导的细胞迁移被抗碱性成纤维细胞生长因子中和抗体抑制，提示其迁移作用依赖于碱性成纤维细胞生长因子。此外，尽管丝裂霉素C抑制了人牙髓细胞的增殖，但仍能观察到碱性成纤维细胞生长因子促进的细胞迁移。提示碱性成纤维细胞生长因子诱导的人牙髓细胞迁移与细胞增殖无关。综上所述，碱性成纤维细胞生长因子可能通过调节细胞外基质的产生和细胞的迁移来调节创面的愈合。在Beagle犬四前牙牙髓暴露部位应用碱性成纤维细胞生长因子可促进bard组织的形成。因此，碱性成纤维细胞生长因子是牙本质-牙髓复合体再生治疗的有用试剂。