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Neuroendcrine study of the affect stress that it originates in the masticatory function

源于咀嚼功能的情感应激的神经内分泌研究

基本信息

批准号：
14370642
负责人：
IKEDA Yasuhiro
金额：
$ 6.91万
依托单位：
Health sciences university of hokkaido
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2003
项目状态：
已结题

项目摘要

Recently, it attracts great deal of masticatory function and the relation with the health of the whole body. Relation with the decline of the masticatory function and psychologic factor is pointed out of that. We planned the hypothesis that a change to the powder breeding feed from the intake of food contrary to the habit, that is, the solid breeding feed caused a stress response by using the rat. Then, the measurement of Aderenocorticotropichormone (ACTH) and Corticosterone (CORT) that a main part is played was done. 140 male Wistar rats (16 weeks old) were divided into two separate groups : a control group (fed with a solid diet) and a soft diet group (fed with a powder diet containing the same components as the solid one). And, a rat used the cage which improvement was added to not to chew the one except for the feed during the experiment. It drew blood from the external jugular vein for 7 days by 10 rats and every day from 17 weeks at each of 8 p.m. After centrifugalize was done, ACTH and CORT in the plasma were measured as for the blood which it could get. Measurement used RIA method for CORT and IRMA method for ACTH. As for the control group, the tendency that it corresponded to rise in the ACTH level or a descent and it of the CORT level was recognized as the result. But, as for the soft diet group, the tendency that a change in the ACTH level grew big after the fourth day was recognized. Being the condition that the normal mutual relationship of the negative feedback by the lower hormone in HPA axis was lost was suggested from this thing. The possibility that it became the stress that the intake of food contrary to the habit was mental was suggested from the result of this research.

近年来，它引起了人们对咀嚼功能及其与全身健康的关系的极大关注。并指出其与咀嚼功能下降及心理因素的关系。我们计划了一个假设，即改变粉末饲养饲料的摄入量与习惯相反，即固体饲养饲料通过使用大鼠引起应激反应。然后对其中起主要作用的促肾上腺皮质激素(ACTH)和皮质酮(CORT)进行测定。将140只16周龄雄性Wistar大鼠随机分为两组：对照组(喂固体饲料)和软饲料组(喂固体饲料中含有相同成分的粉末饲料)。并且，一只大鼠在实验过程中使用了改进后的笼子，除了饲料外，不再咀嚼笼子。10只大鼠连续7天从颈外静脉采血，从17周开始每天晚上8点采血。离心后测定血浆中ACTH和CORT的含量。CORT采用放射免疫法测定，ACTH采用IRMA法测定。对照组ACTH水平上升或皮质醇水平下降的趋势被认为是结果。但是，对于软饮食组，ACTH水平在第四天后变化较大的趋势被认识到。由此推测HPA轴的低激素负反馈失去了正常的相互关系。这项研究的结果表明，与习惯相反的食物摄入是精神上的压力，这可能成为压力。