Neuroendcrine study of the affect stress that it originates in the masticatory function

近年来，咀嚼功能及其与全身健康的关系引起了人们的广泛关注。并指出其与咀嚼功能下降及心理因素有关。我们计划的假设是，从与习惯相反的食物摄入改变为粉末饲养饲料，即固体饲养饲料通过使用大鼠引起应激反应。然后测定其中起主要作用的促肾上腺皮质激素（ACTH）和皮质酮（CORT）。将140只雄性Wistar大鼠（16周龄）分为两组：对照组（饲喂固体饲料）和软饲料组（饲喂含有与固体饲料相同成分的粉末饲料）。而且，一只老鼠使用了改进的笼子，在实验过程中除了饲料外不咀嚼笼子。10只大鼠颈外静脉采血，连续7天，从17周开始每天晚上8点采血，离心后取血测定血浆ACTH和CORT。皮质酮（CORT）测定采用放射免疫分析法（RIA），促肾上腺皮质激素（ACTH）测定采用放射免疫分析法（IRMA）。对于对照组，其对应于ACTH水平升高或CORT水平下降的趋势被认为是结果。但是，对于软质饮食组，发现ACTH水平的变化在第4天之后变大的趋势。由此提出HPA轴低激素负反馈的正常相互关系丧失的条件。从这项研究的结果可以看出，与习惯相反的食物摄入可能成为精神压力。

Recently, it attracts great deal of masticatory function and the relation with the health of the whole body. Relation with the decline of the masticatory function and psychologic factor is pointed out of that. We planned the hypothesis that a change to the powder breeding feed from the intake of food contrary to the habit, that is, the solid breeding feed caused a stress response by using the rat. Then, the measurement of Aderenocorticotropichormone (ACTH) and Corticosterone (CORT) that a main part is played was done. 140 male Wistar rats (16 weeks old) were divided into two separate groups : a control group (fed with a solid diet) and a soft diet group (fed with a powder diet containing the same components as the solid one). And, a rat used the cage which improvement was added to not to chew the one except for the feed during the experiment. It drew blood from the external jugular vein for 7 days by 10 rats and every day from 17 weeks at each of 8 p.m. After centrifugalize was done, ACTH and CORT in the plasma were measured as for the blood which it could get. Measurement used RIA method for CORT and IRMA method for ACTH. As for the control group, the tendency that it corresponded to rise in the ACTH level or a descent and it of the CORT level was recognized as the result. But, as for the soft diet group, the tendency that a change in the ACTH level grew big after the fourth day was recognized. Being the condition that the normal mutual relationship of the negative feedback by the lower hormone in HPA axis was lost was suggested from this thing. The possibility that it became the stress that the intake of food contrary to the habit was mental was suggested from the result of this research.