Neuroendcrine study of the affect stress that it originates in the masticatory function

近年来，人们对咀嚼功能及其与全身健康的关系进行了大量的研究。指出了咀嚼功能衰退与心理因素的关系。我们计划的假设是，从与进食习惯相反的食物中改变粉状饲养饲料，即使用固体饲养饲料引起大鼠的应激反应。然后，对起主要作用的肾上腺皮质激素（ACTH）和皮质酮（CORT）进行了测定。将140只16周龄雄性Wistar大鼠分为两组：对照组（饲喂固体饲料）和软性饲料组（饲喂与固体饲料成分相同的粉状饲料）。在实验过程中，一只大鼠使用添加了改良物的笼子，除了饲料外，不咀嚼笼子。连续7天，10只大鼠颈外静脉抽血，从17周开始每天晚上8点抽血。离心后，测定血浆中ACTH和CORT的含量。CORT采用RIA法测定，ACTH采用IRMA法测定。对照组ACTH水平升高或CORT水平下降的趋势被认为是结果。但是，对于软饮食组，在第四天之后，ACTH水平的变化趋势变得很大。由此提示HPA轴下激素负反馈的正常相互关系丧失。研究结果表明，与习惯相反的食物摄入可能是心理上的压力。

Recently, it attracts great deal of masticatory function and the relation with the health of the whole body. Relation with the decline of the masticatory function and psychologic factor is pointed out of that. We planned the hypothesis that a change to the powder breeding feed from the intake of food contrary to the habit, that is, the solid breeding feed caused a stress response by using the rat. Then, the measurement of Aderenocorticotropichormone (ACTH) and Corticosterone (CORT) that a main part is played was done. 140 male Wistar rats (16 weeks old) were divided into two separate groups : a control group (fed with a solid diet) and a soft diet group (fed with a powder diet containing the same components as the solid one). And, a rat used the cage which improvement was added to not to chew the one except for the feed during the experiment. It drew blood from the external jugular vein for 7 days by 10 rats and every day from 17 weeks at each of 8 p.m. After centrifugalize was done, ACTH and CORT in the plasma were measured as for the blood which it could get. Measurement used RIA method for CORT and IRMA method for ACTH. As for the control group, the tendency that it corresponded to rise in the ACTH level or a descent and it of the CORT level was recognized as the result. But, as for the soft diet group, the tendency that a change in the ACTH level grew big after the fourth day was recognized. Being the condition that the normal mutual relationship of the negative feedback by the lower hormone in HPA axis was lost was suggested from this thing. The possibility that it became the stress that the intake of food contrary to the habit was mental was suggested from the result of this research.