The reaction mechanism of general anesthetics studied by electron spin resonance and fluorescence measurement technique

电子自旋共振和荧光测量技术研究全身麻醉药的反应机理

• 批准号: 14370653
• 负责人: SUZUKI Kuniaki
• 金额: $ 8.77万
• 依托单位: HOKKAIDO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14370653/
• 关键词: liposome; electron spin resonance; order parameter; rotational correlation time; spin label reagent; general anesthetics; membrane protein; Na, K-ATPase; 5-DSA; 16-DSA; ESR; オーダーパラメーター; メタノール; SDS; Na, K-ATPase; タンパク質; 脂質; NMR; 蛍光; 局所麻酔

We investigated the effects of general anesthetics and the temperature on electron spin resonance (ESR) spectra of multilamellar liposome (MLV). Liposomes were composed of DMPC, DOPC or EYPC, labeled with 5-DSA or 16-DSA. The ESR spectra were measured at different temperatures from 10 to 50 ℃. The spectra of MLV labeled by 16-DSA showed three sharp signals compared with 5-DSA. The difference of order parameter S value of 5-DSA and 16-DSA suggested that the nitroxide radical location of 5-DSA and 16-DSA were different in the membrane bilayr. The anesthetics did not alter the S and τ values, which are index of the fluidity of membrane. Therefore it is suggested that anesthetics remain on the surface of lipid bilayr and do not act both the inside hydrophobic area and relatively surface hydrophilic area that the nitroxide radical of spin label located. The anesthetics decreased S and τ values in the low temperature range, suggesting an increase of membrane fluidity, but the effects decreased as the temperature rose. These results suggest that the fluidity of liposome membrane increases with rise of the temperature irrespective of the presence of anesthetics and that the anesthetics increases the fluidity of the membrane especially at the low temperature and shifts the phase transition temperature of saturated fatty acid to a lower temperature.

本文研究了全身麻醉药和温度对多层脂质体（MLV）电子自旋共振（ESR）谱的影响。脂质体由DMPC、DOPC或EYPC组成，分别用5-DSA或16-DSA标记。在10 ~ 50 ℃不同温度下测定了ESR谱。16-DSA标记的MLV与5-DSA标记的MLV相比，其光谱呈现三个尖锐信号。5-DSA和16-DSA的序参量S值的差异表明，5-DSA和16-DSA的氮氧自由基在膜双层中的位置不同。麻醉药对膜流动性指标S和τ值无明显影响。因此，麻醉剂保留在脂质双层表面，而不作用于自旋标记氮氧自由基所处的内部疏水区和表面相对亲水区。麻醉药在低温范围内使S和τ值降低，表明膜流动性增加，但随着温度升高，这种作用减弱。这些结果表明，脂质体膜的流动性随温度的升高而增加，而与麻醉剂的存在无关，并且麻醉剂增加了膜的流动性，特别是在低温下，并使饱和脂肪酸的相变温度向较低温度移动。

项目成果

Yoshiaki Deyama: "The effects of histamine on cyclooxygenase-2 induction in osteoblastic MC3T3-E1 cells"Dentistry in Japan. 39. 40-42 (2003)

Yoshiaki Deyama：“组胺对成骨细胞 MC3T3-E1 细胞中环氧合酶 2 诱导的影响”日本牙科。

The relation between interleukin-6 production by oral squamous cell carcinomas and bone invasion

口腔鳞状细胞癌产生白细胞介素6与骨侵袭的关系

• 发表时间: 2004
• 期刊: Hokkaido Journal of Dental Science 25(2)
• 作者: Kuniaki Maekawa et al.

Necessity of enzymatic activity of alkaline phosphatase for mineralization of osteoblastic cells

• DOI: 10.1254/jjp.88.262
• 发表时间: 2002-03-01
• 期刊: JAPANESE JOURNAL OF PHARMACOLOGY
• 作者: Sugawara, Y;Suzuki, K;Iida, J
• 通讯作者: Iida, J

Hydrostatic pressure stimulates the fusion process in osteoclasts

静水压力刺激破骨细胞的融合过程

• 发表时间: 2004
• 期刊: Dentistry in Japan 40
• 作者: Yoshimura Y;Kikuiri;T;Deyama Y;Hasegawa T;Hatta M;Suzuki K
• 通讯作者: Suzuki K

Mitsutoki Hata: "Regulation of alkaline phosphatase promoter activity by forkhead transcription factor FKHR"International Journal of Molecular Medicine. 9(2). 147-152 (2002)

Mitsutoki Hata：“叉头转录因子 FKHR 对碱性磷酸酶启动子活性的调节”国际分子医学杂志。