Gene therapy targeting cell adhesion molecule that regulate metastasis of oral cancer

针对调节口腔癌转移的细胞粘附分子的基因治疗

• 批准号: 14370674
• 负责人: HAYASHIDO Yasutaka
• 金额: $ 7.04万
• 依托单位: HIROSHIMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14370674/
• 关键词: oral cancer; squamous cell carcinoma; integrin; cell adhesion; invasion; metastasis; 蛋白分解酵素

Integrins are cell adhesion molecules that play an important role in cell spreading, migration and growth. Altered expression of integrin α v β 3 has been known to contribute to the invasion and melastasis in several malignant tumors. To examine the role of integrin α v subunit in the progression of squamous cell carcinoma (SCC), oral SCC cell line SCCKN was stably transfected with sense or antisense α v cDNA. α v-overexpression in SCCKN cells facilitated the proliferation and MMP-2 binding at the cell surface, and seemed to enhance the ability to invade type I collagen gel. In contrast, the cells transfected with antisense α v cDNA showed the decreased the proliferative activity.In conclusion, the results indicated that α v plays a role in the progression of SCC by regulating the growth and the proteolytic activity at the cell surface. The inhibition of α v-expression in oral SCC cells might suppress their invasion and metastasis.

整合素是细胞粘附分子，在细胞的伸展、迁移和生长中起重要作用。整合素α v β 3的表达改变与多种恶性肿瘤的侵袭和转移有关。为探讨整合素α v亚基在口腔鳞癌发生发展中的作用，将α v正、反义cDNA稳定转染口腔鳞癌细胞系SCCKN。α v过表达促进SCCKN细胞增殖和MMP-2在细胞表面的结合，并似乎增强了侵袭I型胶原凝胶的能力。结果表明，α v基因通过调控细胞的生长和细胞表面蛋白水解活性，在SCC的发生发展中起重要作用。抑制口腔鳞癌细胞α v-1的表达可能会抑制其侵袭和转移。

项目成果

Kitano, H.: "Participation of integrin α v in invasive growth of oral squamous cell carcinoma cells"Jpn. J. Tissue Cult. Dent. Res. 11(1). 11-18 (2002)

Kitano，H.：“整合素αv参与口腔鳞状细胞癌细胞的侵袭性生长”J.Tissue Cult.11(1)。

Asada, N., Tanaka, Y., Hayashido, Y., Toratani, S., Kan, M., Kitamoto, M., Nakanishi, T., Kajiyama, G., Chayama, K., Okamoto, T.: "Expression of fibroblast growth factor (FGF) receptor genes in human hepatoma-derived cell lines"In Vitro Cell Dev Biol Anim

Asada, N.、Tanaka, Y.、Hayashido, Y.、Toratani, S.、Kan, M.、Kitamoto, M.、Nakanishi, T.、Kajiyama, G.、Chayama, K.、Okamoto, T.：

Hayashido, Y., Nakashima, M., Urabe, K., Yoshioka, H., Yoshioka, Y., Hamana, T., Kitano, H., Koizumi, K., Okamoto, T.: "Role of stromal thrombospondin-1 in motility and proteolytic activity of oral squamous cell carcinoma cell"Int J Mol Med.. 12(4). 447-4

Hayashido, Y.、Nakashima, M.、Urabe, K.、Yoshioka, H.、Yoshioka, Y.、Hamana, T.、Kitano, H.、Koizumi, K.、Okamoto, T.：“基质血小板反应蛋白的作用

Y.Hayashido, K.Urabe et al.: "Participation of fibroblasts in MMP-2 binding and activation on the surface of oral squamous cell carcinoma cells"Int. J. Oncol.. 22. 657-662 (2003)

Y.Hayashido、K.Urabe 等人：“成纤维细胞参与口腔鳞状细胞癌细胞表面 MMP-2 的结合和激活”Int。

Hayashido Y, Urabe K, Yoshioka Y, Kitano H, Okamoto T, Matsuya T.: "Participation of fibroblasts in MMP-2 binding and activation on the surface of oral squamous cell carcinoma cells"Int. J. Oncol.. 22(3). 657-662 (2003)

Hayashido Y、Urabe K、Yoshioka Y、Kitano H、Okamoto T、Matsuya T.：“成纤维细胞参与口腔鳞状细胞癌细胞表面 MMP-2 结合和激活”Int。