Gene therapy targeting cell adhesion molecule that regulate metastasis of oral cancer

整合素是细胞黏附分子，在细胞的扩散、迁移和生长过程中发挥重要作用。已知整合素αvβ3的表达改变与多种恶性肿瘤的侵袭和转移有关。为探讨整合素αv亚单位在口腔鳞状细胞癌发生发展中的作用，将正义或反义αv基因稳定导入口腔鳞状细胞癌细胞系SCCKN。αv的过表达促进了细胞表面的增殖和MMP2的结合，似乎增强了细胞对I型胶原凝胶的侵袭能力。反义αv基因转染细胞后，细胞增殖活性下降，提示αv基因可能通过调节细胞表面的生长和蛋白降解活性而参与鳞癌的发生发展。抑制口腔鳞癌细胞αv的表达可能抑制其侵袭和转移。

Integrins are cell adhesion molecules that play an important role in cell spreading, migration and growth. Altered expression of integrin α v β 3 has been known to contribute to the invasion and melastasis in several malignant tumors. To examine the role of integrin α v subunit in the progression of squamous cell carcinoma (SCC), oral SCC cell line SCCKN was stably transfected with sense or antisense α v cDNA. α v-overexpression in SCCKN cells facilitated the proliferation and MMP-2 binding at the cell surface, and seemed to enhance the ability to invade type I collagen gel. In contrast, the cells transfected with antisense α v cDNA showed the decreased the proliferative activity.In conclusion, the results indicated that α v plays a role in the progression of SCC by regulating the growth and the proteolytic activity at the cell surface. The inhibition of α v-expression in oral SCC cells might suppress their invasion and metastasis.