Study of roles of epithelial cell rests of Malassez in periodontal regeneration

Malassez上皮细胞残渣在牙周再生中的作用研究

• 批准号: 14370711
• 负责人: OGAWA Tetsuji
• 金额: $ 8.06万
• 依托单位: HIROSHIMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14370711/
• 关键词: epithelial cell rests of Malassez; cementum repair; periodontal tissue regeneration; 培養細胞

n vivo, to clarify the roles of epithelial cell rests of Malassez (ECRM), ECRM existed in periodontal ligament during cementum repair was investigated using morphological and immunohistochemicai approaches in experimental root resorption. At day 7, after mechanical injury, root resorption was observed and ECRM were present adjacent to the site of resorption lacunae. They were observed in periodontal ligament adjacent to site of the resorption lacunae. These ECRM were immunoreactive for bone morphogenetic protein-2. During the stage of early cementum repair, the ECRM were immunoreactive for osteopontin and ameloblastin. These findings suggested that ECRM may be related to cementum repair by activating their potential to secrete matrix proteins which have been expressed in tooth development.In vitro, we investigated the expression of alkaline phosphatase (ALPase), osteopontin (OPN), bone morphogenetic protein (BMP)-2 and BMP-4 in epithelial cells (E cells) and fibroblastic cells (F cells) derived from human periodontal ligament. Reverse transcription-polymerase chain reaction analysis showed that E cells have lower ALPase and BMP-4 mRNA levels than F cells. On the other hand, the expression of OPN mRNA in E cells was stronger than in F cells. Thus, they have different expression patterns of ALPase, BMP-4 and OPN, suggesting that ERM and mesenchymal cells in periodontal ligament may be cooperatively involved in cementum repair.

在体内，为了阐明Malassez上皮细胞（ECRM）在牙周膜修复过程中的作用，采用形态学和免疫组织化学方法研究了ECRM在牙周膜根吸收中的作用。机械损伤后第7天，观察到牙根吸收，在吸收腔隙附近出现ECRM。在靠近吸收腔隙的牙周韧带处可见。这些ECRM对骨形态发生蛋白-2有免疫反应。在早期骨水泥修复阶段，ECRM对骨桥蛋白和成釉细胞蛋白具有免疫反应。这些发现表明，ECRM可能通过激活牙骨质分泌基质蛋白的潜力与牙骨质修复有关，这些基质蛋白在牙齿发育过程中表达。体外实验研究了碱性磷酸酶（ALPase）、骨桥蛋白（OPN）、骨形态发生蛋白(BMP)-2和BMP-4在人牙周韧带上皮细胞（E细胞）和成纤维细胞（F细胞）中的表达。逆转录聚合酶链反应分析显示，E细胞的ALPase和bmp - 4mrna水平低于F细胞。另一方面，OPN mRNA在E细胞中的表达强于F细胞。因此，它们具有不同的ALPase、BMP-4和OPN表达模式，提示牙周膜ERM和间充质细胞可能协同参与牙骨质修复。

项目成果

Mouri Y, Shiba H, Mizuno N, Noguchi T, Ogawa T, Kurihara H.: "Differential gene expression of bone-related proteins in epithelial and fibroblastic cells derived from human periodontal ligament"Cell Biol Int.. 22(7). 519-524 (2003)

Mouri Y、Shiba H、Mizuno N、Noguchi T、Okawa T、Kurihara H.：“人牙周韧带来源的上皮细胞和成纤维细胞中骨相关蛋白的差异基因表达”Cell Biol Int.. 22(7)。

Mouri Y.: "Differential gene expression of bone-related proteins in epithelial and fibroblastic cells derived from human periodontal ligament"Cell Biol Int,. 22(7). 519-524 (2003)

Mouri Y.：“人牙周膜来源的上皮细胞和成纤维细胞中骨相关蛋白的差异基因表达”Cell Biol Int，。

Mouri Y, Shiba H, Mizuno N, Noguchi T, Ogawa T, Kurihara H.: "Differential gene expression of bone-related proteins in epithelial and fibroblastic cells derived from human periodontal ligament."Cell Biol Int.. 22(7). 519-524 (2003)

Mouri Y、Shiba H、Mizuno N、Noguchi T、Okawa T、Kurihara H.：“人牙周膜来源的上皮细胞和成纤维细胞中骨相关蛋白的差异基因表达。”Cell Biol Int.. 22(7)。

Hasegawa N: "lmmunohistochemical characteristics of epithelial cell rests of Malassez during cementum repair"Journal of Periodontal of Research. 38. 51-56 (2003)

长谷川 N：“牙骨质修复过程中马拉塞兹上皮细胞的免疫组织化学特征”牙周研究杂志。

長谷川直彦, 小川哲次他: "Immunohistochemical characteristics of epithelial cell rests of Malassez durnig cementum repair"Journal of Periodontal Research. 38. 51-56 (2003)

Naohiko Hasekawa、Tetsuji Okawa 等人：“Malassez 牙骨质修复过程中上皮细胞残骸的免疫组织化学特征”，牙周研究杂志 38. 51-56 (2003)。