STUDY OF INDUCTIVE CALCIFIED TISSUE REGENERATION WITH CULTURED PELIODONTAL LIGAMENT CELL

1;应用免疫组织化学方法探讨了非胶原基质蛋白在破牙髓细胞、成牙骨质细胞和新生牙骨质再生过程中的定位。结果表明骨桥蛋白和骨钙蛋白参与了骨质再生的早期钙化，骨钙蛋白、骨连接素和BMP-2参与了后期钙化。2;我们检测了马拉色氏根吸收到牙骨质再生期间上皮细胞的免疫组织化学定位。马拉塞人改变了他们的结构，似乎在这一时期发挥了一些作用。我们认为Malassez在骨质再生中有另一个作用。3;为了进一步阐明马拉塞兹上皮细胞休止在骨质再生中的作用，我们检测了马拉塞兹的超微结构。在根吸收期，马拉塞兹与发育期相似。我们认为Malassez有可能在骨水泥形态变化的再生中发挥作用。4;我们研究了破牙细胞的超微结构以及OPN和BSP在牙根吸收中的定位。结果表明，破牙细胞与成骨细胞相似，根吸收的发生机制与骨吸收相似。然而，在早期骨质再生中，非胶原基质蛋白的定位与骨重塑不同。

1 ; We discussed localization of non-collagenous matrix protein on odontoclast, cementoblast and new cementum during cementum regeneration by means of immunohistochemistry. Results suggest that osteopontin and bone sialo protein participate in early calcification of cementum regeneration and that osteocalcin, osteonectin and BMP-2 have roles in later calcification.2 ; We examined immunohistochemically localization of epithelial cell rests of Malassez in period from root resorption to cementum regeneration. Malassez chage their structure and seems to have some roles in this period. We propose that Malassez have another role in cementum regeneration.3 ; To elucidate detail role of epithelial cell rests of Mallasez, we examined ultrastructure of Malassez in cementum regeneration. In period of root resorption Malassez is similar to in development. We propose that Malassez have possibility to play roles in cementum regeneration with changing form.4 ; We investegated ultrastructure of odontoclast and localization of OPN and BSP in root resorption. Results shows that odontoclast is similar to osteiclast and that root resorption occurs in similar mechanism to bone resorption. However, in early cementum regeneration localization of non-collagenous matrix protein is differ from bone remodeling.