STUDY OF INDUCTIVE CALCIFIED TISSUE REGENERATION WITH CULTURED PELIODONTAL LIGAMENT CELL

1.应用免疫组织化学方法，探讨破牙本质细胞、成牙骨质细胞和新生牙骨质在牙骨质再生过程中非胶原基质蛋白的定位。结果提示，骨桥蛋白和骨唾液蛋白参与了牙骨质再生的早期钙化过程，骨钙素、骨连接蛋白和骨形态发生蛋白-2在后期钙化过程中起作用。2.应用免疫组织化学方法检测了牙根吸收到牙骨质再生过程中马拉色氏上皮细胞的定位。马拉塞兹改变了他们的结构，似乎在这一时期扮演了一些角色。我们认为Malassez在牙骨质再生中还有另一个作用。3.为了阐明Mallasez上皮细胞残基在牙骨质再生中的作用，我们观察了Malassez在牙骨质再生中的超微结构。在根吸收时期，马拉色斯与发育期相似。我们认为Malassez有可能通过改变形态在牙骨质再生中发挥作用。4.我们研究了破牙细胞的超微结构以及OPN和BSP在牙根吸收中的定位。结果表明，破牙本质细胞与成骨细胞相似，牙根吸收的发生机制与骨吸收相似。然而，在牙骨质再生的早期，非胶原基质蛋白的定位不同于骨改建。

1 ; We discussed localization of non-collagenous matrix protein on odontoclast, cementoblast and new cementum during cementum regeneration by means of immunohistochemistry. Results suggest that osteopontin and bone sialo protein participate in early calcification of cementum regeneration and that osteocalcin, osteonectin and BMP-2 have roles in later calcification.2 ; We examined immunohistochemically localization of epithelial cell rests of Malassez in period from root resorption to cementum regeneration. Malassez chage their structure and seems to have some roles in this period. We propose that Malassez have another role in cementum regeneration.3 ; To elucidate detail role of epithelial cell rests of Mallasez, we examined ultrastructure of Malassez in cementum regeneration. In period of root resorption Malassez is similar to in development. We propose that Malassez have possibility to play roles in cementum regeneration with changing form.4 ; We investegated ultrastructure of odontoclast and localization of OPN and BSP in root resorption. Results shows that odontoclast is similar to osteiclast and that root resorption occurs in similar mechanism to bone resorption. However, in early cementum regeneration localization of non-collagenous matrix protein is differ from bone remodeling.