STUDY OF INDUCTIVE CALCIFIED TISSUE REGENERATION WITH CULTURED PELIODONTAL LIGAMENT CELL

培养牙周韧带细胞诱导钙化组织再生的研究

• 批准号: 08457507
• 负责人: OGAWA Tetsuji
• 金额: $ 4.29万
• 依托单位: HIROSHIMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08457507/
• 关键词: REPLANTATION; CULTURED PELIODONTAL LIGAMENT CELL; CEMENTUM REGENERATION; IMMUNOCYTOLOGY; HISTOLOGY

1 ; We discussed localization of non-collagenous matrix protein on odontoclast, cementoblast and new cementum during cementum regeneration by means of immunohistochemistry. Results suggest that osteopontin and bone sialo protein participate in early calcification of cementum regeneration and that osteocalcin, osteonectin and BMP-2 have roles in later calcification.2 ; We examined immunohistochemically localization of epithelial cell rests of Malassez in period from root resorption to cementum regeneration. Malassez chage their structure and seems to have some roles in this period. We propose that Malassez have another role in cementum regeneration.3 ; To elucidate detail role of epithelial cell rests of Mallasez, we examined ultrastructure of Malassez in cementum regeneration. In period of root resorption Malassez is similar to in development. We propose that Malassez have possibility to play roles in cementum regeneration with changing form.4 ; We investegated ultrastructure of odontoclast and localization of OPN and BSP in root resorption. Results shows that odontoclast is similar to osteiclast and that root resorption occurs in similar mechanism to bone resorption. However, in early cementum regeneration localization of non-collagenous matrix protein is differ from bone remodeling.

1个;我们用免疫组化方法探讨了牙骨质再生过程中非胶原基质蛋白在破牙本质细胞、成牙骨质细胞和新生牙骨质上的定位。结果表明，骨桥蛋白和骨唾液酸蛋白参与早期钙化的牙骨质再生和骨钙素，骨连接素和BMP-2有作用，在以后的钙化。2;我们研究了上皮细胞休息的Malassez从根吸收到牙骨质再生的化学定位。马拉色改变了他们的结构，似乎在这一时期有一些作用。我们建议，Malassez有另一个作用，在牙骨质再生。3;为了阐明详细的作用，上皮细胞休息的Mallasez，我们研究了超微结构的Malassez在牙骨质再生。在牙根吸收期，Malassez与发育期相似。我们认为Malassez可能通过改变形态在牙骨质再生中发挥作用。4.我们研究了破牙本质细胞的超微结构以及OPN和BSP在牙根吸收中的定位。结果表明，破牙细胞与破骨细胞相似，牙根吸收机制与骨吸收机制相似。然而，在牙骨质再生早期，非胶原基质蛋白的定位与骨重建不同。