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Gene Polymorphism of Urine Protein 1 and its Clinical Application.

尿蛋白1基因多态性及其临床应用。

基本信息

批准号：
14370791
负责人：
ITOH Yoshihisa
金额：
$ 4.61万
依托单位：
Asahikawa Medical College
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2004
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14370791/
关键词：
Protein 1 SNPs CC 10 IgA nephropathy 尿中安定性 PCR法 LAMP法 SNP uteroglobin 肺クララ細胞10kDa蛋白遺伝子多型

项目摘要

Protein 1 (p1), also called as Clara cell 10 kDa protein, or uteroglobin, is nonglycoprotein with a molecular weight of 14 kDa with anti-inflammatory function. Employing alleic PCR, direct sequencing, luciferase reporter assay, ELISA, immunohistochemical staining the authors has shown the following new data.1.The frequency of G38AA is 2 times higher in IgA nephropathy than normal individuals. Decrease of the uteroglobin value in the patients' sera may indicate that G38A may be a predisposing factor for the diseases as a result of relative decrease of anti-inflammatory effects.2.The G38A gene polymorphism was also investigated in patients with pulmonary sarcoidosis. 38A predominance was found in those with poor prognosis. The value in serum and BALF is decreased. To support this, production of P1 was decreased in interferon-gamma-stimulated culture system, which was demonstrated by luciferase assay.3.In secretary phase P1 was most expressed in normal epithelia, to less degree in endometriosis. In endometrial cancer P1 expression has lost. Precise mechanism is unclear, however, regulation of progesterone becomes ineffective in cancer.4.Stability of P1 in acidic urine was theoretically evaluated. Structurally the cleavage site on the molecule by urine enzymes are only two. Furthermore, conformity is probably protected by hydrophobic cavity where different ligands are trapped preventing the molecule from being digested.5.A LAMP assays and microflow devices are being developed for simple and rapid detection of SNPs on P1.

蛋白1（Protein 1，p1）又称克拉拉细胞10 kDa蛋白，或子宫珠蛋白，是一种分子量为14 kDa的非糖蛋白，具有抗炎作用。本研究采用等位基因PCR、直接测序、荧光素酶报告基因分析、ELISA、免疫组化等方法，获得了以下新的结果：1.伊加肾病患者G38 AA的频率是正常人的2倍。结论：（1）G38 A基因多态性与肺结节病的发病密切相关，且与肺结节病的发病密切相关。（2）G38 A基因多态性与肺结节病的发病密切相关。预后不良者38 A占优势。血清和BALF中的值降低。荧光素酶检测结果表明，干扰素-γ刺激的培养体系中P1的表达量明显减少。3.分泌期P1在正常上皮细胞中表达最多，在子宫内膜异位症中表达较少。在子宫内膜癌中，P1表达丢失。确切的机制尚不清楚，然而，孕酮的调节在癌症中变得无效。4.从理论上评估了P1在酸性尿液中的稳定性。从结构上讲，尿酶在分子上的切割位点只有两个。此外，一致性可能受到疏水腔的保护，在疏水腔中捕获不同的配体，防止分子被消化。5.LAMP检测和微流装置正在开发用于简单快速检测P1上的SNP。