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Molecular mechanisms of appendage formation ; genes involved in the outgrowth, differentiation of appendage formation

附属物形成的分子机制；

基本信息

批准号：
14380348
负责人：
YAMADA Gen
金额：
$ 3.14万
依托单位：
Kumamoto University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2003
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14380348/
关键词：
external genitalia reproduction shh (sonic hedgehog)fgf (fibroblast growth factor)bmp (bone morphogenetic protein)fin appendage growth factor マウス遺伝子ノックアウトマウス BMP 生殖 FGF

项目摘要

Internal fertilization is an efficient form of reproduction, but requires male and female anatomical specializations. While external fertilization is the general rule for fish, certain cartilaginous and bony fish reproduce by internal fertilization, having an anal fin modified into a copulatory organ, the gonopodium. Most birds copulate by placing their cloacas in contact for the transfer of sperm, but some avian species possess elaborate male copulatory organs. All mammals utilize internal fertilization, and as a consequence ; reproductive efficiency is increased.As for mammals (mouse), we have shown already shh (sonic hedgehog)-/-mutant mice displayed complete external genitalia agenesis suggesting its importance for the "developmental field" formation as cloaca. In this study, we have shown that in avian system, shh expression is also observed during cloaca formation. It was shown by this study that Bmp may function during cloaca formation which may interact with shh pathway. With the help of our recent established technique, sonoporation, we are further analyzing functions of various factors for cloaca formation in birds. For fish system, we have shown that shh is expressed and functions in the distal region of gonopodium, as copulatory organ derived from anal fin. We have shown that Shh functions for the outgrowth regulation of such gonopodium. These studies suggest the importance of some "shared developmental mechanisms for copulatory organ formation" in diverged animal species.

体内受精是一种有效的生殖方式，但需要雄性和雌性的解剖学特化。虽然体外受精是鱼类的一般规则，但某些软骨和骨性鱼类通过内部受精繁殖，其肛鳍被改造成交配器官，即生殖器。大多数鸟类通过将泄殖腔放在一起进行交配来传递精子，但一些鸟类拥有精心制作的雄性交配器官。所有哺乳动物都利用内部受精，结果是繁殖效率提高。至于哺乳动物(小鼠)，我们已经表明Shh(声波刺猬)/-突变小鼠表现出完全的外生殖器发育不全，这表明它对于作为泄殖腔的“发育场”的形成具有重要意义。在本研究中，我们发现在禽类系统中，在泄殖腔形成过程中也观察到shh的表达。本研究表明，BMP可能在泄殖腔形成过程中发挥作用，并可能与Shh途径相互作用。借助于我们最近建立的技术，Sonopation，我们正在进一步分析各种因素在鸟类泄殖腔形成中的作用。对于鱼类系统，我们已经证明Shh在生殖器的远端区域表达和功能，作为来自肛鳍的交配器官。我们已经证明Shh在这种生殖器的生长调节中起作用。这些研究表明，在不同的动物物种中，一些“交配器官形成的共同发育机制”是重要的。