Basic Researches on the Micropositioning techniques of Cells and Biomaterials for Tissue Engineering

组织工程细胞与生物材料微定位技术基础研究

• 批准号: 14380393
• 负责人: NAKAMURA Makoto
• 金额: $ 11.07万
• 依托单位: Tokyo Medical and Dental University, Institute of Biomaterials and Bioengineering
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14380393/
• 关键词: Tissue Engineering; Organs for transplantation; Engineered vascular tissues; Biomaterials; inkjet; Bio-artificial organs; Micro manipulator; Bioprinting; Pantograph; Micro manipulator; Pantograph mechanism

Biological tissues and organs are constructed with a microscopic structure, with several types of cells and extra cellular matrixes, in three dimensionally. Therefore, it is very important in the field of tissue engineering to establish the technologies to position living cells and biological materials precisely onto an intentionally targeted position.In this research project, we have been aiming to make basic researches and to produce some innovative technologies to position living cells onto the targeted position, to contribute to develop tissue engineering. As some of the promising approaches, we have investigated the feasibility of micropositioning techniques of living cells and biomaterials such as using inkjet printing and micromanipulation techniques. And we summarized the concept and discussed about the possibilities of these approaches for tissue engineering.We focused mainly on the inkjet techniques for tissue engineering. We have challenged to eject living cells using inkjet … More machine, and have investigated the damages of the ejected cells. As a result, we have recognized that we could eject living cells without significant damages. To avoid drying of cells and to construct three-dimensional structures, we developed a gel formation technique by inkjet printing. With this technique, we could prevent cells from drying and succeeded in the construction of three dimensional gel structures such as a lattice and a tube with fine gel fibers, including living cells in them. Such techniques for micropositioning of cells and biomaterials enables us to manufacture biological tissues with the techniques of computer aided designing and manufacturing. Innovative tissue engineering will come true.Then, we designed a microvasculature pattern and evaluated it using the technique of computational fluid dynamics. In addition, we constructed computational three-dimensional model of organs from a clinical CT scan data, and made some three dimensional cast models based on such CAD data using three-dimensional printer.In these ways, we have made basic researches on the micropositioning techniques of cells and biomaterials for tissue engineering and have demonstrated the possibility of these approaches using computer and micropositioning techniogies. Collaboration of advanced computer and mechanical technologies with tissue engineering will surely contribute to the future of tissue engineering medicine. Less

生物组织和器官是由几种类型的细胞和细胞外基质以三维方式构建的微观结构。因此，在组织工程领域，建立将活细胞和生物材料精确定位到目标位置的技术是非常重要的。本研究课题的目的是进行基础研究，并提出一些将活细胞定位到目标位置的创新技术，为组织工程的发展做出贡献。作为一些有前途的方法，我们已经研究了活细胞和生物材料的微定位技术的可行性，如使用喷墨打印和微操作技术。本文对组织工程的概念进行了总结，并对这些方法在组织工程中的应用进行了探讨，重点介绍了喷墨技术在组织工程中的应用。我们挑战用喷墨打印机喷射活细胞 ...更多信息 机器，并调查了弹出细胞的损害。因此，我们已经认识到，我们可以在没有重大损害的情况下排出活细胞。为了避免细胞干燥并构建三维结构，我们开发了通过喷墨打印的凝胶形成技术。通过这种技术，我们可以防止细胞干燥，并成功地构建了三维凝胶结构，如网格和具有细凝胶纤维的管，其中包括活细胞。这种细胞和生物材料的微定位技术使我们能够利用计算机辅助设计和制造技术制造生物组织。然后，我们设计了一种微血管模型，并利用计算流体力学技术对其进行了评价。此外，我们还利用临床CT扫描数据建立了器官的计算机三维模型，并利用三维打印机制作了器官的三维模型，为组织工程中细胞和生物材料的微定位技术进行了基础性研究，论证了利用计算机和微定位技术进行组织工程中细胞和生物材料微定位的可能性。先进的计算机和机械技术与组织工程学的结合必将为组织工程医学的发展做出贡献。少

项目成果

Computer Aided Tissue Engineering : インクジェットによる二次元生体組織プリンティング.

计算机辅助组织工程：使用喷墨进行二维生物组织打印。

• 发表时间: 2004
• 期刊: 生体医工学 42(suppl.1)
• 作者: 中村真人;他
• 通讯作者: 他

Designing of the Capillary Vasculature using micro-CFD

使用微 CFD 设计毛细血管系统

• 发表时间: 2003
• 期刊: The Japanese Journal of Artificial Organs 32(2)
• 作者: Nakamura M;et al.
• 通讯作者: et al.

中村真人, 森田育男, 他: "インクジェットによる細胞マイクロ播種法の研究"人工臓器. 32(2). S-67 (2003)

Masato Nakamura、Ikuo Morita 等：“使用喷墨的细胞微接种方法的研究”Artificial Organs 32(2)。

Nakamura M, Morita I, et al.: "Micro Cell Seeding with Living Cells using a Bio-Compatible Inkjet Printing Technique."2003 Conference Abstracts ; 6th. Annual international conference and exposition of Tissue Engineering Society International. (CD-ROM). P0

Nakamura M、Morita I 等人：“使用生物相容性喷墨打印技术用活细胞进行微细胞接种。”2003 年会议摘要；

Inkjet technique for the formation of alginate hydrogel containing living cells.

用于形成含有活细胞的藻酸盐水凝胶的喷墨技术。

• 发表时间: 2004
• 期刊: Abstract Booklet of Bioprinting and Biopatterning Workshop 2004
• 作者: Nakamura M;Kobayashi A;et al.
• 通讯作者: et al.