Development of a Phase-Modulation Fluorometer and a Fluorescence-Lifetime Mapping System and ~ Its Application for Tumor Diagnosis Using a Microscope

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We have developed a phase-modulation fluorometer and other spectroscopic instruments for obtain specially, spectrally, and temporally resolved information of biological samples. Our final goal is to discriminating a tumor cell from a normal one under a microscope. In order to realize such a system, we have developed the following instrumental techniques : a Fourier-transform phase-modulation fluorometer, a sinusoidally-modulation method of a xenon lamp, a nanosecond-pulse-driven ultraviolet LED, a nanosecond-pulse-driven white LED, a photomultiplier-tube internal-gating method for use in subnanosecond time-resolved spectroscopy, a pseudo-lock-in light detection method for a gain-modulated photomultiplier tube, and so on. In combination with an ultraviolet semiconductor laser and a fluorescence microscope, we have measured fluorescence decay waveform for some biological samples