Development of a Phase-Modulation Fluorometer and a Fluorescence-Lifetime Mapping System and ~ Its Application for Tumor Diagnosis Using a Microscope

相位调制荧光计和荧光寿命映射系统的开发及其在显微镜肿瘤诊断中的应用

• 批准号: 14380407
• 负责人: IWATA Tetsuo
• 金额: $ 8.58万
• 依托单位: The University of Tokushima
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14380407/
• 关键词: Fluorescence lifetime; Phase modulation method; Microscopy; Spatial mapping; Spectroscopic measurement; Tumor diagnosis

We have developed a phase-modulation fluorometer and other spectroscopic instruments for obtain specially, spectrally, and temporally resolved information of biological samples. Our final goal is to discriminating a tumor cell from a normal one under a microscope. In order to realize such a system, we have developed the following instrumental techniques : a Fourier-transform phase-modulation fluorometer, a sinusoidally-modulation method of a xenon lamp, a nanosecond-pulse-driven ultraviolet LED, a nanosecond-pulse-driven white LED, a photomultiplier-tube internal-gating method for use in subnanosecond time-resolved spectroscopy, a pseudo-lock-in light detection method for a gain-modulated photomultiplier tube, and so on. In combination with an ultraviolet semiconductor laser and a fluorescence microscope, we have measured fluorescence decay waveform for some biological samples

为了获得生物样品的空间、光谱和时间分辨信息，我们研制了相位调制荧光计和其他光谱仪器。我们的最终目标是在显微镜下区分肿瘤细胞和正常细胞.为了实现这样的系统，我们开发了以下仪器技术：傅里叶变换相位调制荧光计、氙灯的正弦调制方法、纳秒脉冲驱动的紫外LED、纳秒脉冲驱动的白色LED、用于亚纳秒时间分辨光谱学的光电倍增管内选通方法、用于增益调制光电倍增管的伪锁定光检测方法结合紫外半导体激光器和荧光显微镜，我们测量了一些生物样品的荧光衰减波形

项目成果

T.Iwata, T.Takasu, T.Araki: "Simple Photomultiplier-Tube Internal-Gating Method for Use in Subnanosecond Time-Resolved Spectroscopy"Applied Spectroscopy. Vol.57, No.9. 1145-1150 (2003)

T.Iwata、T.Takasu、T.Araki：“用于亚纳秒时间分辨光谱的简单光电倍增管内门方法”应用光谱。

T.Iwata, T.Inoue, T.Araki: "Pseudo-Lock-in Light Detection Method for a Gain-Modulated Photomultiplier-Tube"Optical Review. 11・1. 19-23 (2004)

T.Iwata、T.Inoue、T.Araki：“增益调制光电倍增管的伪锁定光检测方法”光学评论 11・1 (2004)。

T.Iwata, T.Takasu, T.Araki: "Simple Photomultiplier-Tube Internal-Gating Method for Use in Subnanosecond Time-Resolved Spectroscopy"Applied Spectroscopy. 57・9. 1145-1150 (2003)

T.Iwata、T.Takasu、T.Araki：“用于亚纳秒时间分辨光谱的简单光电倍增管内选通方法”应用光谱学 57・9。

T.Iwata, T.Inoue, T.Araki: "Pseudo-Lock-in Light Detection Method for a Gain-Modulated Photomultiplier-Tube"Optical Review. Vol.11, No.1. 19-23 (2004)

T.Iwata、T.Inoue、T.Araki：“增益调制光电倍增管的伪锁定光检测方法”光学评论。

T.Iwata: "Proposal for Fourier-Transform Phase-Modulation Fluorometer"Optical Review. 10・1. 31-37 (2003)

T.Iwata：“傅里叶变换相位调制荧光计的提案”光学评论 10・1 (2003)。