Development of instrumental methods and systems for fluorescence lifetime measurements for a screening purpose of tumor diagnosis

为了提高肿瘤细胞与正常细胞的鉴别能力，对荧光寿命测量的仪器和数据处理方法进行了全面的研究。我们的最终目标是发展诊断计量学。在一系列的研究中，我们开发了一种使用调相光源的调相荧光计，一种光子计数型调相荧光计，以及一种使用射频锁定放大器的近红外荧光计。通过使用这些系统，我们精确地测量了吲哚菁绿溶液和6 1×10^<-6> M香豆素152在乙醇中的荧光寿命。

Comprehensive studies have been carried out concerning on instrumental and data-processing methods for fluorescence lifetime measurements with a purpose of enhancing capabilities of discriminating tumor cells from normal cells. Our final goal is developing diagnostic metrology. In a series of studies, we have developed a phase-modulation fluorometer using a phase-modulated light source, a photon-counting-type phase-modulation fluorometer, and a near-infrared fluorometer using a radio-frequency lock-in amplifier. By using these systems, we have measured a fluorescence lifetime of the indocyanine green solution and that of 6 1×10^<-6> M coumarin 152 in ethanol with precision.