Development of instrumental methods and systems for fluorescence lifetime measurements for a screening purpose of tumor diagnosis

为了提高肿瘤细胞与正常细胞的区分能力，对荧光寿命测量的仪器和数据处理方法进行了全面的研究。我们的最终目标是发展诊断计量学。在一系列的研究中，我们已经开发了一个相位调制荧光计使用相位调制光源，光子计数型相位调制荧光计，和近红外荧光计使用射频锁定放大器。利用这些系统，我们精确地测量了吲哚菁绿色溶液和6 1×10^<-6>M香豆素152乙醇溶液的荧光寿命。

Comprehensive studies have been carried out concerning on instrumental and data-processing methods for fluorescence lifetime measurements with a purpose of enhancing capabilities of discriminating tumor cells from normal cells. Our final goal is developing diagnostic metrology. In a series of studies, we have developed a phase-modulation fluorometer using a phase-modulated light source, a photon-counting-type phase-modulation fluorometer, and a near-infrared fluorometer using a radio-frequency lock-in amplifier. By using these systems, we have measured a fluorescence lifetime of the indocyanine green solution and that of 6 1×10^<-6> M coumarin 152 in ethanol with precision.