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Construction of a Fourier-transform Phase-Modulation Fluorometer and a Fluorescence-Lifetime Mapping System and Its Application for Tumor Diagnosis Using a Microscope

傅里叶变换相位调制荧光计和荧光寿命映射系统的构建及其在肿瘤肿瘤诊断中的应用

基本信息

批准号：
16300157
负责人：
IWATA Tetsuo
金额：
$ 8.77万
依托单位：
The University of Tokushima
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2005
项目状态：
已结题

项目摘要

We have constructed a Fourier-transform phase-modulation fluorometer (FT-PMF) by which fluorescence decay waveform can be obtained. To demonstrate the performance of the FT-PMF, we carried out (1)measurement of a fluorescent decay wave form of YAG materials enclosed in a white LED and (2)determinations of fluorescence lifetimes of 10 ppm quinine sulfate in 0.1 N H_2SO_4 and 10 ppm rhodamine 6G in ethanol. Our final goal is to discriminating a tumor cell from a normal one by using difference in the values of the fluorescence lifetimes. The FT-PMF was, therefore, combined with an inverted fluorescence-microscope to obtain specially, spectrally, and temporally resolved information for biological samples. Fundamental results for demonstrating the system performance have been obtained. On the one hand, we have developed a pseudo-lock-in light detection system using a gain-enhanced gated silicon avalanche photodiode, which is applied for a pulse oximeter. Also, we have proposed a new extrapolation method using an iterative Hilbert-transform applicable for band-limited frequency data obtained from the FT-PMF. We are now going to continue the project to reach the final goal by using the developed techniques.

我们研制了一种傅里叶变换相位调制荧光计（FT-PMF），利用它可以获得荧光衰减波形。为了证明FT-PMF的性能，我们进行了（1）测量封装在白色LED中的YAG材料的荧光衰减波形和（2）测定在0.1 N H_2SO_4中的10 ppm硫酸奎宁和在乙醇中的10 ppm罗丹明6 G的荧光寿命。我们的最终目标是通过荧光寿命值的差异来区分肿瘤细胞和正常细胞。因此，FT-PMF与倒置荧光显微镜相结合，以获得生物样品的特殊，光谱和时间分辨信息。得到了验证系统性能的基本结果。一方面，我们利用增益增强型门控硅雪崩光电二极管研制了一种伪锁定光检测系统，并将其应用于脉搏血氧仪。此外，我们提出了一种新的外推方法，使用迭代希尔伯特变换适用于有限频带的频率数据从FT-PMF。我们现在将继续该项目，通过使用开发的技术达到最终目标。