Construction of a Fourier-transform Phase-Modulation Fluorometer and a Fluorescence-Lifetime Mapping System and Its Application for Tumor Diagnosis Using a Microscope

我们构建了傅里叶变换相位调制荧光仪(FT-PMF)，利用它可以获得荧光衰减波形。为了证明FT-PMF的性能，我们进行了(1)测量白光LED封装的YAG材料的荧光衰减波形和(2)测量了10ppm硫酸奎宁在0.1N H_2SO_4和10ppm罗丹明6G在乙醇中的荧光寿命。我们的最终目标是通过荧光寿命值的不同来区分肿瘤细胞和正常细胞。因此，FT-PMF与倒置荧光显微镜相结合，以获得生物样品的特殊的、光谱的和时间分辨的信息。给出了验证系统性能的基本结果。一方面，我们利用增益增强型门控硅雪崩光电二极管研制了一种用于脉搏血氧仪的伪锁相光探测系统。此外，我们还提出了一种新的外推方法，该方法使用迭代希尔伯特变换，适用于从FT-PMF获得的带限频率数据。我们现在将继续这个项目，通过使用开发的技术来达到最终目标。

We have constructed a Fourier-transform phase-modulation fluorometer (FT-PMF) by which fluorescence decay waveform can be obtained. To demonstrate the performance of the FT-PMF, we carried out (1)measurement of a fluorescent decay wave form of YAG materials enclosed in a white LED and (2)determinations of fluorescence lifetimes of 10 ppm quinine sulfate in 0.1 N H_2SO_4 and 10 ppm rhodamine 6G in ethanol. Our final goal is to discriminating a tumor cell from a normal one by using difference in the values of the fluorescence lifetimes. The FT-PMF was, therefore, combined with an inverted fluorescence-microscope to obtain specially, spectrally, and temporally resolved information for biological samples. Fundamental results for demonstrating the system performance have been obtained. On the one hand, we have developed a pseudo-lock-in light detection system using a gain-enhanced gated silicon avalanche photodiode, which is applied for a pulse oximeter. Also, we have proposed a new extrapolation method using an iterative Hilbert-transform applicable for band-limited frequency data obtained from the FT-PMF. We are now going to continue the project to reach the final goal by using the developed techniques.