Synthesis of Structured Phospholipids Containing Polyunsaturated Fatty Acid and Their Functional Evaluation

多不饱和脂肪酸结构磷脂的合成及其功能评价

• 批准号: 15360438
• 负责人: YAMANE Tsuneo
• 金额: $ 9.79万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15360438/
• 关键词: phosphatidylserine; phospholipase D; structured phospholipid; docosahexaenoic acid; AAMI; neural cultured cell; amyloid β protein; neural toxin; ホスフォリパーゼD; リパーゼ; 加齢に伴う記憶力減退; 神経毒性

It has been recently reported that both phosphatidylserine (a kind of natural phospholipids) and docosahexaenoic acid (DHA, a kind of polyunsaturared fatty acids) have positive effects on human brain neural system. Because of them, they have attracted much the attention of people as functional foods or medical materials that contribute to brain health. The objective of this work is to synthesize a structured phosphatidylserine whose fatty acid residue is DHA, and to evaluate its function in cultured neural cells.1)Starting from phosphatidylcholine which is the most abundant phspholipid in nature, three kinds of structured phospholipids have been synthesized chomo-enzymatically : i.e., by using enzymes (phospholipases A_2 and D) combined with chemical deacylation and acylation (esterification). They are 1,2-didocosahexaenoyl-phosphatidylcholine(DDC), 1,2-didocosahexaenoyl-phsphatidylserine (DBS), and 1-oleoyl-2-docosahexaenoyl-phosphatidylserine(ODS).2)Brain neural cells were first prepared from rat fetal brain and cultured in DMEM/F-12 mixed medium. Adding various compounds including the synthesized phospholipids, protective or deteriorating effect on the cell death in the culture was evaluated by determining activity of lactose dehydrogenase released from apoptosis of the cells. Also, their protecting effect was evaluated from neuron damage induced by amyloid β protein in the culture.3)Depressive effects of DDC,DDS and ODS were not observed on natural cell death caused in the culture condition. However, it was observed that DDC,DDS and ODS protected from the neuron damage induced by added amyloid β1-42 protein.

近年来研究发现，天然磷脂磷脂酰丝氨酸（phosphatidylserine，PI）和多不饱和脂肪酸二十二碳六烯酸（docosahexaenoic acid，DHA）均对人脑神经系统有积极作用。正因为如此，它们作为有助于大脑健康的功能性食品或医用材料引起了人们的广泛关注。本工作的目的是合成一种脂肪酸残基为DHA的结构化磷脂酰丝氨酸，并评价其在培养的神经细胞中的功能。1）以磷脂酰胆碱为原料，采用化学-酶法合成了三种结构化磷脂：用酶（磷脂酶A_2和D）结合化学脱酰和酰化（酯化）。它们是1，2-二二十二碳六烯酰磷脂酰胆碱（DDC）、1，2-二二十二碳六烯酰磷脂酰丝氨酸（DBS）和1-油酰-2-二十二碳六烯酰磷脂酰丝氨酸（ODS）。通过测定细胞凋亡释放的乳糖脱氢酶的活性，评价加入包括合成磷脂在内的各种化合物对培养物中细胞死亡的保护或恶化作用。3）DDC、DDS和ODS对培养条件下引起的细胞自然死亡无抑制作用。DDC、DDS和ODS对β1-42淀粉样蛋白诱导的神经元损伤有保护作用。

项目成果

An aqueous suspension system for phospholipase D-mediated synthesis of phosphatidylserine without toxic organic solvent.

一种用于磷脂酶 D 介导的磷脂酰丝氨酸合成的水悬浮系统，无需有毒有机溶剂。

• 发表时间: 2003
• 期刊: Journal of the American Oil Chemists' Society 80(7)
• 作者: Yugo Iwasaki;Yukiko Mizumoto;Takahiro Okada;Takaya Yamamoto;Kentaro Tsutsumi;Tsuneo Yamane
• 通讯作者: Tsuneo Yamane

セリンの回収方法

如何回收丝氨酸

• 发表时间: 2004

An aqueous suspension system for phospholipase-D-mediated synthesis of phosphatidylserine without organic solvent

一种用于磷脂酶 D 介导的无有机溶剂合成磷脂酰丝氨酸的水悬浮系统

• 发表时间: 2003
• 期刊: Journal of the American Oil Chemists' Society 80(7)
• 作者: Yugo Iwasaki;Tsuneo Yamane;Yugo Iwasaki 他4名;Yugo Iwasaki et al.
• 通讯作者: Yugo Iwasaki et al.

Yugo Iwasaki et al.: "An Aqueous Suspension System for Phospholipase D-Mediated Synthesis of PS Without Toxic Organic Solvent"Juournal of American Oil Chemists' Society. 80・7. 653-657 (2003)

Yugo Iwasaki 等人：“用于磷脂酶 D 介导的 PS 合成的水悬浮系统，无需有毒有机溶剂”，美国石油化学家协会杂志 80・7（2003 年）。

Detection of phospholipase D on solid materials

固体材料上磷脂酶 D 的检测

• 发表时间: 2004
• 期刊: Analytical Biochemistry 329
• 作者: 西浜章平;吉塚和治;Iwasaki Y.et al.
• 通讯作者: Iwasaki Y.et al.