Ultra-highly effective utilization of lipid resources-Enzymatic syntheses of structured lipids-

1.作为本项目的前提条件，我们成功地利用银离子高效液相色谱和手性高效液相色谱相结合的方法分离和测定了对称结构的三酰甘油ABA(式中A=正辛酸，B=多不饱和脂肪酸PUFA)和不对称结构的AAB(和/或BAA)。我们还成功地分离和测定了手性二酰甘油AA(OH)和(OH)AA2。利用固定化南极假丝酵母脂肪酶对鱼油进行乙醇解，再与固定化米氏里佐毛霉脂肪酶进行酯化反应，可以非常有效地合成对称结构的三酰甘油(ABA)。以含13%二十二碳六烯酸的蛋黄卵磷脂和过量的L丝氨酸为原料，通过磷脂酶D的转磷脂酰化作用，成功地合成了磷脂酰丝氨酸。

1. As a prerequisite to this project, we succeeded in separation and determination of symmetrical structured triacylglycerol, ABA (where A=n-octanoic acid, B=a polyunsaturated fatty acid, PUFA), and asymmetrical ones, AAB (and/or BAA) by use of a combination of silver ion HPLC and chiral HPLC. We also succeeded in the separation and determination of chiral diacylglycerols, AA (OH) and (OH) AA.2. Symmetrical structured triacylglycerol, ABA, was able to be synthesized very effectively by a two-step method which was composed of ethanolysis of bonito oil with immobilized Candida antarctica lipase, and then esterification of the resulting monoacylglycerol, (OH) (PUFA) (OH) with immobilized Rizomucor miehei lipase.3. From egg yolk lecithin containing 13% docosahexaenoic acid and excessive L-serine, phosphatidylserine was able to be synthesized successfully by the transphosphatidylation action of phospholipase D.