Ultra-highly effective utilization of lipid resources-Enzymatic syntheses of structured lipids-

脂质资源超高效利用-酶法合成结构化脂质-

• 批准号: 12839004
• 负责人: YAMANE Tsuneo
• 金额: $ 1.98万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12839004/
• 关键词: structured lipid; chiral lipid; lipase; phospholipase D; polyunsaturated fattyacid acid; transesterifiation; diacylglycerol; phosphatidylserine; DHA; EPA

1. As a prerequisite to this project, we succeeded in separation and determination of symmetrical structured triacylglycerol, ABA (where A=n-octanoic acid, B=a polyunsaturated fatty acid, PUFA), and asymmetrical ones, AAB (and/or BAA) by use of a combination of silver ion HPLC and chiral HPLC. We also succeeded in the separation and determination of chiral diacylglycerols, AA (OH) and (OH) AA.2. Symmetrical structured triacylglycerol, ABA, was able to be synthesized very effectively by a two-step method which was composed of ethanolysis of bonito oil with immobilized Candida antarctica lipase, and then esterification of the resulting monoacylglycerol, (OH) (PUFA) (OH) with immobilized Rizomucor miehei lipase.3. From egg yolk lecithin containing 13% docosahexaenoic acid and excessive L-serine, phosphatidylserine was able to be synthesized successfully by the transphosphatidylation action of phospholipase D.

1.作为该项目的前提，我们成功地分离和测定对称结构的三酰甘油，阿坝（其中A=正辛酸，B=多不饱和脂肪酸，PUFA），和不对称的，AAB（和/或BAA）的组合使用的银离子HPLC和手性HPLC。我们还成功地分离和测定了手性甘油二酯，AA（OH）和（OH）AA。采用固定化南极假丝酵母脂肪酶对鲣鱼油进行醇解，再用固定化米黑根毛霉脂肪酶对单酰基甘油（OH）（PUFA）（OH）进行酯化，可以有效地合成对称结构的三酰基甘油（阿坝）.以含有13%二十二碳六烯酸和过量L-丝氨酸的蛋黄卵磷脂为原料，利用磷脂酶D的转磷脂酰化作用，成功地合成了磷脂酰丝氨酸。

项目成果

Yugo Iwasaki, Tsuneo Yamane: "Enzymatic synthesis of structured lipids"Journal of Molecular Catalysis B : Enzymatic. 10・9. 129-140 (2000)

岩崎佑吾、山根恒雄：“结构化脂质的酶促合成”《分子催化杂志 B》：酶学 10・9（2000 年）。

Tsuneo Yamane and Yugo Iwasaki: "Functional Lipids and Biotechnology"Biosrience and Industry. 58. 837-844 (2000)

Tsuneo Yamane 和 Yugo Iwasaki：“功能性脂质和生物技术”生物科学与工业。

Roxana Irimescu: "Utilization of reaction medium-dependent regiospecificity of Candida antarctica lipase for the synthesis of 1,3-dicapryloy1-2-DHA-glycerol"J. Am. Oil Chem. Soc.. 78・3. 285-289 (2001)

Roxana Irimescu：“利用南极假丝酵母脂肪酶的反应介质依赖性合成 1,3-二辛酰 1-2-DHA-甘油”J. Oil Chem. 2001。 ）

Roxana Irimescu: "Two-step enzymatic synthesis of docosahexaenoic acid-rich symmetrically structured triacylglycerols via 2-monoacylglycerols"J. Am. Oil Chem. Soc.. 78・7. 743-748 (2001)

Roxana Irimescu：“通过 2-单酰基甘油两步酶法合成富含二十二碳六烯酸的三酰基甘油”J. Oil Chem. 78・748。

R.Irimescu et al: "Two-step enzymatic synthesis of docosahexaenoic acid-rich symmetrically structuted triacyl-glycerols via 2-monoglycerols"Journal of the American Oil Chemists' Society. 78. 743-748 (2001)

R.Irimescu 等人：“通过 2-单甘油两步酶促合成富含二十二碳六烯酸的对称结构三酰基甘油”美国石油化学家协会杂志。