Kinetics of Microaqueous Bioreactor System

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To establish the kinetics of enzynatic reaction in microaqueous organic solvent, quantitative investigations were performed on the states of water molecules in the microaqueous organic solvent, and on two different types of enzymatic reactions. The interesting results mentioned below were obtained.1. Experimental procedure was established to determine separately protein-bound water and free water dissolved in organic solvent. Normal languimier-type ad-sorption isotherms were observed between the protein-bound water and the free water dissolved in water-miscible organic solvents, while abnormal monotonically rising isotherms were obtained between the protein-bound water and the free water dissolved in water-limmiscible organic solvents. Adsorption isotherms depend also on the purity of enzyme proteins, implying that water binds also impurities existing in the enzyme preparations.2. Both batch and continuous glycerolysis of fat by lipase between two liquid phases consisting glycerik phas … More e and liquid fat phase were studied. The initial reaction rate of decreasing triglyceride content increased with the power of 1.7 of moisture content. the free fatty acid hardly formed below 4% moisture content in the glycerol phase, but started to form significantly above 4%. Half-life of the lipase was about 21 days at 40゜c. Fat peroxide not only inhibited the glycerolysis reaction but also inactivated it, but they were not serious.3. Intramolecular esterification (lactonization) reaction of 15-hydroxypentadecanoic acid by lipase power suspended in microaqueous benzene was studied. Effects of moisture (both bound- and free -water) content on both initial reaction rate and product yield were made clear. The reaction rates increased first and decreased then with the increase in moisture content, giving rise to a maximum rate at a particular moisture content. The yield increased to almost 100% at decreasing moisture content. It was concluded that the reaction was limited by hydration of the enzyme protein at lower moisture content, while it was limited by true reversible reaction at elevated moisture content. Less