Comparative analysis of "chromosome for pathogenicity" in phytopathogenic fungi

植物病原真菌“致病性染色体”的比较分析

• 批准号: 15380037
• 负责人: YAMAMOTO Mikihiro
• 金额: $ 8.7万
• 依托单位: OKAYAMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15380037/
• 关键词: Black spot of strawberry; Alternaria blotch of apple; Alternaria stem canker of tomato; Alternaria alternate; CD chromosome; BAC library; cDNA library; Host-specific toxin

We have found, in Alternaria alternata, productivity of host-specific toxin(HST) controls pathogenicity on plants, genes listed so far were specifically involved in metabolic pathway of synthesis for each of or a group of HST, and genes involved in HST production located only on a small chromosome that was conditionally dispensable. In this research, we focused on comparative analysis of structure and function of conditionally dispensable chromosome(CD chromosome) among pathotypes of A.alternata to understand molecular basis for evolution and differentiation of plant pathogen.1.We constructed three sets of CD chromosome-specific BAC library and cDNA library from three pathotypes of A.alternata (apple, strawberry and tomato pathotype). Clones of interests were subjected to DNA sequencing and genes involved in HST biosynthesis were identified.2.Sixteen genes located around 300-kb region were identified for HST biosynthesis of strawberry pathotype. Two genes were identified in apple pathotype and thirteen genes located around 150-kb region were also identified in tomato pathotype, respectively for each HST.3.One gene for aldo-keto reductase was conserved between CD chromosomes of apple and strawberry pathotype while apple pathotype produces a HST containing cyclic peptide and strawberry pathotype produces HSTs containing epoxy-decatrienoic acid (EDT). The introduced homolog of strawberry pathotype could functionally complement knocked out function of the other homolog in apple pathotype.4.Any genes for HST biosynthesis were not conserved between CD chromosomes of apple and/or strawberry pathotype and tomato pathotype that produces a HST containing polyketide moiety.These results revealed CD chromosomes of apple and strawberry may be derived from an ancient chromosome but that of tomato may be not although three pathotypes are in the same species A.alternata and suggest a novel example of differentiation of phytopathogenic fungi.

我们发现，在替代替代品中，宿主特异性毒素（HST）的生产力控制植物上的致病性，到目前为止列出的基因专门参与了每种或一组HST的代谢途径，以及仅在HST产量中涉及的基因，仅位于有条件可分配的小染色体上。在这项研究中，我们专注于对A.Alternata病原体的有条件可分配染色体（CD染色体）的结构和功能的比较分析，以了解植物病原体的进化和分化的分子基础。1。我们构建了三组CD染色体染色体BAC BAC图书馆和CDNA图书馆和CDNA图书馆的三个Pathotypes，来自三个Pathotypes A.Alternataata（Apple apple apple，Stroperrys，Stropryry，Stropy）。对感兴趣的克隆进行了DNA测序，并鉴定了与HST生物合成有关的基因。2。鉴定了位于300 kb区域的十六个基因，用于草莓病原体的HST生物合成。 Two genes were identified in apple pathotype and thirteen genes located around 150-kb region were also identified in tomato pathotype, respectively for each HST.3.One gene for aldo-keto reductase was conserved between CD chromosomes of apple and strawberry pathotype while apple pathotype produces a HST containing cyclic peptide and strawberry pathotype produces HSTs containing epoxy-decatrienoic acid （美东时间）。引入的草莓病原体同源物可能在功能上可以补充苹果病原体中其他同源物的功能。4。在苹果和/或草莓病原体和/或草莓病原体和番茄病原体的CD染色体之间，任何用于HST生物合成的基因都不保守尽管不是三种病原体在同一物种中，但提出了分化植物致病真菌的新例子。

项目成果

Genomic And Genetic Analysis Of Plant Parasitism And Defense

• 发表时间: 2003-12
• 作者: S. Tsuyumu;J. Leach;白石 友紀;T. Wolpert

分子レベルからみた植物の耐病性

分子水平上的植物抗病性

• 发表时间: 2004
• 作者: H.Ishihara;S.Uchida;Y.Masuda;K.Tamura;S.Tsuyumu;豊田和弘
• 通讯作者: 豊田和弘

Structural analysis of cosmid clone pcAFT-2 carrying AFT10-1 encoding an acyl-CoA dehydrogenase involved in AF-toxin production in the strawberry pathotype of Alternaria alternata

• DOI: 10.1007/s10327-004-0170-3
• 发表时间: 2005-04-05
• 期刊: Journal of General Plant Pathology
• 影响因子: 1.2
• 作者: Ruswandi, Sheila;Kitani, Keiji;Yamamoto, Mikihrio
• 通讯作者: Yamamoto, Mikihrio

植物病の探究

植物病害探索

• 发表时间: 2004
• 作者: Tsuge;T.;尾谷 浩
• 通讯作者: 尾谷 浩

Dissection of the host range of the fungal plant pathogen Alternaria alternata by modification of the secondary metabolism

通过修改次生代谢来剖析真菌植物病原体链格孢菌的宿主范围

• 发表时间: 2004
• 期刊: Molecular Microbiology 52(2)
• 作者: Ito;Kaoru
• 通讯作者: Kaoru