Expression pattern and function of chitinase family proteins, endogenous lectins

几丁质酶家族蛋白、内源凝集素的表达模式和功能

• 批准号: 15390052
• 负责人: IWANAGA Toshihiko
• 金额: $ 8.7万
• 依托单位: HOKKAIDO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15390052/
• 关键词: chitinase; AMCase; chitotriosidase; Ym; brp-39; ガレクチン; 好中球; 乳腺

Chitinase family proteins are endogenous lectins which have affinity to N-acetylglucosamine. Two members with chitinase activity, acidic mammalian chitinase (AMCase) and chitotriosidase, have been identified in mammals, and their cellular expressions were investigated in the present study. Furthermore, we selected two chitinase family proteins without the chitinase activity, Ym and brp-39, and revealed their expression sites. AMCase was contained in secretory granules of parotid gland acinar cells and gastric chief cells, secreted in the saliva and gastric juice, respectively. Bovine AMCase, on the other hand, was produced in the liver and released into the blood. A sharp increase of blood AMCase concentration caused by Theileria infection suggested the role in defense against pathogenic bacterial and parasitic infection. Although human chitotriosidase is expressed in macrophages and neutrophils, the mouse counterpart was expressed in the stratified epithelium of oral cavity, suggesting another function of this chitinase. Ym1 was expressed in premature neutropluls in the bone marrow and spleen of mice, but disappeared in mature neutrophils. Macrohages in the bone marrow also contained Ym in association with intracellular needle-like crystals. In the lung, Ym was contained in alveolar macrophages and secreted into alveolar space. In mice infected with M. Corti, inflammatory cells expressing Ym gathered around the parasites residing in the liver, while Ym-containing macrophages in the lung increased in number and changed to multinudeated giant cells. Brp-39 was expressed by chondrocytes at the endochondral ossification of the joint, and by acinar cells in involuted mammary glands. Since mammary glands during lactation never expressed brp-39, this chitinase might be involved in the involution of mammary gland or tissue reconstruction.

几丁质酶家族蛋白是一类与N-乙酰氨基葡萄糖有亲和力的内源性凝集素。在哺乳动物中已鉴定出两个具有几丁质酶活性的成员，即酸性哺乳动物几丁质酶(AMCase)和几丁三糖苷酶，并对它们的细胞表达进行了研究。此外，我们选择了两个不具有几丁质酶活性的几丁质酶家族蛋白Ym和BRP-39，并揭示了它们的表达部位。AMCase存在于腮腺腺泡细胞和胃主细胞的分泌颗粒中，分别在唾液和胃液中分泌。另一方面，牛AMCase在肝脏中产生，并释放到血液中。泰勒氏杆菌感染引起的血液AMCase浓度急剧升高，提示其对病原菌和寄生虫感染具有防御作用。虽然人几丁糖苷酶在巨噬细胞和中性粒细胞中表达，但小鼠的相应几丁质酶在口腔复层上皮细胞中表达，这表明该几丁质酶还有另一种功能。YM1在小鼠骨髓和脾的早熟中性粒细胞中表达，但在成熟的中性粒细胞中消失。骨髓中的大噬细胞也含有YM，与细胞内的针状晶体有关。在肺中，YM存在于肺泡巨噬细胞中，并分泌到肺泡腔中。在感染科尔提分枝杆菌的小鼠中，表达YM的炎症细胞聚集在寄生在肝脏的寄生虫周围，而肺中含有YM的巨噬细胞数量增加，并转变为多结节的巨细胞。BRP-39在关节软骨内骨化的软骨细胞和消退的乳腺中腺泡细胞表达。由于哺乳期乳腺不表达BRP-39，该几丁质酶可能参与乳腺退缩或组织重建。

项目成果

Immunohitochemical demonstration of acidic mammalian chitinase in the mouse salivary gland and gastric mucosa.

小鼠唾液腺和胃粘膜中酸性哺乳动物几丁质酶的免疫组织化学演示。

• 发表时间: 2003
• 期刊: Arch.Oral Biol. 48
• 作者: Y.Kansui;K.Fujii;K.Nakamura;K.Goto;H.Oniki;I.Abe;Y.Shibata;M.Iida;Goto M et al.
• 通讯作者: Goto M et al.

Goto M, et al.: "Immunohistochemical demonstration of acidic mammalian chitinase in the mouse salivary gland and gastric mucosa"Arch.Oral Biol.. 48. 701-707 (2003)

Goto M 等：“小鼠唾液腺和胃粘膜中酸性哺乳动物几丁质酶的免疫组织化学演示”Arch.Oral Biol.. 48. 701-707 (2003)

Purification and identification of a binding protein for pancreatic secretory trypsin inhibitor : a novel role of the inhibitor as an anti-granzyme A.

胰腺分泌性胰蛋白酶抑制剂结合蛋白的纯化和鉴定：该抑制剂作为抗颗粒酶 A 的新作用。

• 发表时间: 2003
• 期刊: Biochem.J. 372
• 作者: T.Inai;M.R.Mancuso;D.M.McDonald;J.Kobayashi;K.Nakamura;Y.Shibata;S.Mori;Naoki Ishiguro. et al.;T.Inai et al.;Tsuzuki S. et al.
• 通讯作者: Tsuzuki S. et al.

Cellular expression of murine Ym1 and Ym2, chitinase family proteins, as revealed by in situ hybridization and immunohistochemistry

• DOI: 10.1007/s00418-004-0654-4
• 发表时间: 2004-06-01
• 期刊: HISTOCHEMISTRY AND CELL BIOLOGY
• 影响因子: 2.3
• 作者: Nio, J;Fujimoto, W;Iwanaga, T
• 通讯作者: Iwanaga, T

Fujimoto W., et al.: "Quantitative changes in serum concentration of bovine gut chitinase in Theileria infection"J.Vet.Med.Sci. 66(印刷中). (2004)

Fujimoto W. 等人：“泰勒虫感染中牛肠道几丁质酶血清浓度的定量变化”J.Vet.Med.Sci. 66（印刷中）。