Existence of PGP9.5 in synoviocytes in horse joint and its application to clinical diagnosis

马关节滑膜细胞中PGP9.5的存在及其在临床诊断中的应用

• 批准号: 12660264
• 负责人: IWANAGA Toshihiko
• 金额: $ 2.3万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12660264/
• 关键词: PGP9.5; immunohistochemistry; ELISA; joint; tendon sheath; 関節炎; 滑膜細胞

We succeeded in selectively staining type B synoviocytes in the joint, bursa synovialis and tendon sheath by use of an antiserum against protein gene product (PGP) 9.5. Furthermore, an application of whole mount preparations to these tissues made entire observation of their distribution and shape possible. Inner surface of the bursa synovialis was covered by PGP9.5-immunoreactive, multinuclear synoviocytes with dendritic processes. Immunoreactive cells in the tendon sheath were densely distributed around mesenterium, but fewer in other regions, where accumulated processes of immunoreactive cells formed a thick layer on the surface layer.The existence of PGP9.5 in synovial fluid was confirmed by immunoblot analysis for PGP9.5. We established an enzyme-linked immunosorbent assay (ELISA) to measure contents of PGP9.5 in the synovial fluid from normal and abnormal joints. PGP9.5 level in normal joints was 0.329±0.033, while it was evaluated as 0.871±0.462 in intra-articular fracture. Although the values for osteochondrosis dissecans (0.701±0.436) and inflammatory arthropathy (0.567±0.132) were not significantly different from the control level, they tended to be increased. Therefore, this assay is useful for diagnosis of intra-articular fracture and suggested that more sensitive assay system using two types of antibodies may be needed for diagnosis of other kinds of joint disorders.

我们成功地选择性染色B型滑膜细胞在关节，滑液囊和腱鞘使用抗血清蛋白基因产物（PGP）9.5。此外，将整体封片制备物应用于这些组织使得可以全面观察它们的分布和形状。滑膜囊内表面覆盖着PGP9.5免疫反应阳性的多核滑膜细胞，具有树突状突起。免疫反应阳性细胞在腱鞘中密集分布于肠系膜周围，但在其他区域较少，在这些区域，免疫反应阳性细胞的积累过程在表层形成了一层厚的层。我们建立了酶联免疫吸附试验（ELISA）来测定正常和异常关节滑液中PGP9.5的含量。正常关节PGP9.5水平为0.329±0.033，关节内骨折PGP9.5水平为0.871±0.462。剥脱性骨软骨病（0.701±0.436）和炎性关节病（0.567±0.132）与对照组相比无显著性差异，但有升高趋势。因此，该测定可用于诊断关节内骨折，并建议可能需要使用两种类型的抗体的更灵敏的测定系统用于诊断其他类型的关节疾病。

项目成果

Iwanaga T. et al.: "Morphology and functional roles of synoviocytes in the joint"Arch. Histol. Cytol.. 63. 17-31 (2000)

Iwanaga T. 等人：“关节中滑膜细胞的形态和功能作用”Arch。

Kitamura H. et al.: "Increased concentrations of protein gene product 9.5 in the synovial fluids from horses with osteoarthritis."Jpn. J. Vet. Res.. 19. 115-123 (2001)

Kitamura H.等人：“患有骨关节炎的马的滑液中蛋白质基因产物9.5的浓度增加。”Jpn。

Kitamura et al.: "Increased concentrations of protein gene product 9.5 in the synovial fluid from horses with osteoarthritis"Jpn.J.Vet.Res.. 49. 115-123 (2001)

Kitamura 等人：“患有骨关节炎的马的滑液中蛋白质基因产物 9.5 的浓度增加”Jpn.J.Vet.Res.. 49. 115-123 (2001)

Iwanaga T.et al: "Morphology and functional roles of synoviocytes in the joint."Arch.Histol.Cytol.. 63. 17-31 (2000)

Iwanaga T.等人：“关节中滑膜细胞的形态和功能作用。”Arch.Histol.Cytol.. 63. 17-31 (2000)

岩永敏彦ら: "滑膜細胞の形態と機能"細胞. 33. 264-267 (2001)

Toshihiko Iwanaga 等：“滑膜细胞的形态和功能”Cell 33. 264-267 (2001)。