Existence of PGP9.5 in synoviocytes in horse joint and its application to clinical diagnosis

我们成功地选择性染色B型滑膜细胞在关节，滑液囊和腱鞘使用抗血清蛋白基因产物（PGP）9.5。此外，将整体封片制备物应用于这些组织使得可以全面观察它们的分布和形状。滑膜囊内表面覆盖着PGP9.5免疫反应阳性的多核滑膜细胞，具有树突状突起。免疫反应阳性细胞在腱鞘中密集分布于肠系膜周围，但在其他区域较少，在这些区域，免疫反应阳性细胞的积累过程在表层形成了一层厚的层。我们建立了酶联免疫吸附法（ELISA）测定正常和异常关节滑液中PGP9.5的含量。正常关节PGP9.5水平为0.329±0.033，关节内骨折PGP9.5水平为0.871±0.462。剥脱性骨软骨病（0.701±0.436）和炎性关节病（0.567±0.132）与对照组相比无显著性差异，但有升高趋势。因此，该测定可用于诊断关节内骨折，并建议可能需要使用两种类型的抗体的更灵敏的测定系统用于诊断其他类型的关节疾病。

We succeeded in selectively staining type B synoviocytes in the joint, bursa synovialis and tendon sheath by use of an antiserum against protein gene product (PGP) 9.5. Furthermore, an application of whole mount preparations to these tissues made entire observation of their distribution and shape possible. Inner surface of the bursa synovialis was covered by PGP9.5-immunoreactive, multinuclear synoviocytes with dendritic processes. Immunoreactive cells in the tendon sheath were densely distributed around mesenterium, but fewer in other regions, where accumulated processes of immunoreactive cells formed a thick layer on the surface layer.The existence of PGP9.5 in synovial fluid was confirmed by immunoblot analysis for PGP9.5. We established an enzyme-linked immunosorbent assay (ELISA) to measure contents of PGP9.5 in the synovial fluid from normal and abnormal joints. PGP9.5 level in normal joints was 0.329±0.033, while it was evaluated as 0.871±0.462 in intra-articular fracture. Although the values for osteochondrosis dissecans (0.701±0.436) and inflammatory arthropathy (0.567±0.132) were not significantly different from the control level, they tended to be increased. Therefore, this assay is useful for diagnosis of intra-articular fracture and suggested that more sensitive assay system using two types of antibodies may be needed for diagnosis of other kinds of joint disorders.