Morphological analysis on paracrine action of brain-gut hormones.

1.为了分析脑肠激素的局部激素样（旁分泌）作用，研究了胃、尿道和气管中肽能神经元的特征性分布。胃主体粘膜主要受含grp的胆碱能神经元支配。尿道粘膜全贴装观察CGRP神经元与血清素内分泌细胞的分布及相互关系。气管上皮内CGRP神经纤维异常丰富。血清素和VIP在腹泻中起重要作用。形态学和生理学研究表明，上皮EC细胞分泌的5 -羟色胺刺激邻近固有层含有VIP的神经元释放VIP，引起严重腹泻。渗透泵局部给药脑肠肽的研究。渗透微泵比微球更适合局部给药。连续、有规律地分别注射GRP和CGRP或P物质于胃本体和气管粘膜。结果表明，肽可引起注射部位的白细胞和肥大细胞浸润。显微镜下的详细分析正在进行中。

1.In order to analyze local hormone-like (paracrine) actions of brain-gut hormones, characteristic distribution of peptidergic neurons was investigated in the stomach, urethra and trachea. The mucosa of the gastric corpus was innervated predominantly by GRP-containing cholinergic neurons. Observation of whole mount preparations of the urethral mucosa revealed entire distribution of CGRP neurons and serotonin endocrine cells and their relationship. The tracheal epithelium was extremely rich in CGRP nerve fibers.2.Physiological study on local hormone-like actions Serotonin and VIP play an important role in diarrhea. Morphological and physiological studies in dogs demonstrated that serotonin secreted from epithelial EC cells stimulated the release of VIP from adjacent VIP-containing neurons in the lamina propria to cause severe diarrhea.3.Local administration of brain-gut peptides by means of osmotic pump. Osmotic micropump was more available than pellets to locally administer the peptides. GRP and CGRP or substance P were continuously and regularly injected to the mucosa of gastric corpus and trachea, respectively. It was shown that the peptides caused infiltration of white blood cells and mast cells at the sites where the peptides were injected. Detailed analysis under microscopes is in progress.