Morphological analysis on paracrine action of brain-gut hormones.

脑肠激素旁分泌作用的形态学分析。

• 批准号: 03670006
• 负责人: IWANAGA Toshihiko
• 金额: $ 1.22万
• 依托单位: Niigata University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1992
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03670006/
• 关键词: Brain-gut peptides; Local hormones; Paracrine secretion; GRP; CGRP; Serotonin; VIP; Diarrhea; セトロニン

1.In order to analyze local hormone-like (paracrine) actions of brain-gut hormones, characteristic distribution of peptidergic neurons was investigated in the stomach, urethra and trachea. The mucosa of the gastric corpus was innervated predominantly by GRP-containing cholinergic neurons. Observation of whole mount preparations of the urethral mucosa revealed entire distribution of CGRP neurons and serotonin endocrine cells and their relationship. The tracheal epithelium was extremely rich in CGRP nerve fibers.2.Physiological study on local hormone-like actions Serotonin and VIP play an important role in diarrhea. Morphological and physiological studies in dogs demonstrated that serotonin secreted from epithelial EC cells stimulated the release of VIP from adjacent VIP-containing neurons in the lamina propria to cause severe diarrhea.3.Local administration of brain-gut peptides by means of osmotic pump. Osmotic micropump was more available than pellets to locally administer the peptides. GRP and CGRP or substance P were continuously and regularly injected to the mucosa of gastric corpus and trachea, respectively. It was shown that the peptides caused infiltration of white blood cells and mast cells at the sites where the peptides were injected. Detailed analysis under microscopes is in progress.

1.为了分析脑肠激素的局部激素样（旁分泌）作用，研究了胃、尿道和气管中肽能神经元的特征分布。胃体粘膜主要由含有 GRP 的胆碱能神经元支配。对尿道粘膜整体标本的观察揭示了 CGRP 神经元和血清素内分泌细胞的完整分布及其关系。气管上皮富含CGRP神经纤维。2.局部激素样作用的生理学研究5-羟色胺和VIP在腹泻中发挥重要作用。对狗的形态学和生理学研究表明，上皮EC细胞分泌的5-羟色胺刺激邻近固有层中含有VIP的神经元释放VIP，引起严重腹泻。3.通过渗透泵局部施用脑肠肽。渗透微泵比颗粒更容易局部施用肽。将GRP和CGRP或P物质分别连续定期注射至胃体和气管粘膜。结果表明，肽在注射肽的部位引起白细胞和肥大细胞的浸润。显微镜下的详细分析正在进行中。

项目成果

Iwanaga,Toshihiko: "GRP-containing nerves in the rat stomach and stress-induced depletion of their synaptic vesicles.An electron microscope study." Arch.Histol.Cytol. 54. 573-580 (1991)

Iwanaga, Toshihiko：“大鼠胃中含有 GRP 的神经和应激诱导的突触小泡耗竭。电子显微镜研究。”

Iwanaga Toshihiko: "Enterochromaffin (EC) cells in the gut with special reference to their role in diarrhea." Biomed. Res.(1993)

Iwanaga Toshihiko：“肠道中的肠嗜铬 (EC) 细胞，特别是它们在腹泻中的作用。”

Iwanaga,Toshihiko: "Enterochromaffin (EC)cells in the gut with special reference to their role in diarrhea." Biomed.Res.(1993)

Iwanaga, Toshihiko：“肠道中的肠嗜铬 (EC) 细胞，特别是它们在腹泻中的作用。”

Terada Masaki: "CGRP-immunoreactive nerve in the tracheal epithelium of rats: an immunohistochemical study by means of whole mount preparations." Arch. Histol. Cytol.55. 219-233 (1992)

Terada Masaki：“大鼠气管上皮中的 CGRP 免疫反应性神经：通过整体制剂进行的免疫组织化学研究。”

Iwanaga, Toshihiko: "Distribution and ultrastructure of nerve fibers in the mucosa of the gastric body." Gut Hormones. 10. 472-476 (1991)

Iwanaga, Toshihiko：“胃体粘膜神经纤维的分布和超微结构。”