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Development of a tumor cell bank for next generation immunotherapy

开发下一代免疫疗法的肿瘤细胞库

基本信息

批准号：
15390380
负责人：
KATANO Mitsuo
金额：
$ 9.34万
依托单位：
KYUSHU UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2004
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15390380/
关键词：
Tumor cells for treatment Tumor cell bank Immunotherapy Vaccine therapy Solid tumors Allo-tumor cell vaccine Exosomes 難治性固形腫瘍

项目摘要

Purpose of this study is to develop a highly successful procedure for establishing tumor cell lines which conform to safety criteria of blood transfusion and to build a system to use these tumor cells for treatment of other humans (a tumor cell bank for treatments).Data obtained during this study period are summarized as follows :1.Establishment of tumor cell lines for treatment :1)We prepared a 96-well culture microplate in which each wells contain different concentrations of autologous sera or effusions. By using this microplate, 6 tumor cell lines were successfully established from 32 fresh carcinoma tissues.2)We succeeded in long-term culture of tumor cells existing in tumor tissue by using micro-gravity culture system.2.Registration of tumor cells for treatment (tumor cell bank) :Among the 6 tumor cell lines, we got registration permission from five patients as a tumor cell bank.Although we got registration permission from other 4 patients, we did not succeed in the establishment of a tumor cell line. These tumor tissues were frozen and stored as associate tumor cells for treatment.3.Clinical application of tumor cell bank :In 2 of 3 patients who are undergoing self tumor-pulsed dendritic cell-based vaccine therapy, it was judged to be able to use non-self tumor cells registered as tumor cell bank instead of self tumor cells.In one patient who used up self tumor cells, the vaccine therapy was continued by using non-self tumor cells registered to tumor cell bank. Through a treatment period, an antitumor immune response was maintained well, and no particular side effect was recognized.4.Publicity of tumor cell bank :We showed clinical data of our dendritic cell-based vaccine therapy and introduced tumor cell bank in a homepage (http://www.tumor.med.kyushu-u.ac.jp/).5. Possibility of cell-free vaccine therapy using GMP-grade exosomes secreted from tumor cells registered to tumor cell bank was indicated.

本研究的目的是建立一种非常成功的方法来建立符合输血安全标准的肿瘤细胞系，并建立一个使用这些肿瘤细胞用于治疗其他人的系统（用于治疗的肿瘤细胞库）。2）利用微重力培养系统成功地对肿瘤组织中存在的肿瘤细胞进行了长期培养。2.肿瘤细胞库的注册：在6种肿瘤细胞系中，我们获得了5名患者的肿瘤细胞库注册许可，另外4名患者获得了肿瘤细胞库注册许可，但我们没有成功建立肿瘤细胞系。3.肿瘤细胞库的临床应用：在3例接受自体肿瘤致敏树突状细胞疫苗治疗的患者中，有2例经判断可以使用肿瘤细胞库中登记的非自体肿瘤细胞代替自体肿瘤细胞，其中1例自体肿瘤细胞用完后，继续使用肿瘤细胞库中登记的非自体肿瘤细胞进行疫苗治疗。4.肿瘤细胞库的宣传：在主页（http：//www.tumor.med.kyushu-u.ac.jp/）上展示了我们的树突状细胞疫苗疗法的临床数据，并介绍了肿瘤细胞库。指出了使用从登记到肿瘤细胞库的肿瘤细胞分泌的GMP级外泌体进行无细胞疫苗治疗的可能性。