Effect of Basal Ganglia upon Epileptic Seizures in Animal Mode

动物模式下基底神经节对癫痫发作的影响

• 批准号: 15390427
• 负责人: TANAKA Tatsuya
• 金额: $ 4.22万
• 依托单位: School of Medicine Asahikawa medical college
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15390427/
• 关键词: Kainic acid; Basak ganglia; Globus pallidus; Deep brain stimulation; Intractable Epilepsy; Experimental epilpesy; 視床下核; 深部脳電気刺激; てんかん発作; ムシモール

Inhibitory and excitatory effects of basal ganglia and subthalamic nuclei were studied using kainic acid -induced seizure model in rats. Cortical epileptogenic foci were induced by intracortical microinjections of kainic acid. In Experiment 1, rats with a cortical focus, induced seizures were well suppressed by high frequency electrical stimulation of bilateral subthalamic nuclei (STN). In experiment 2, muscimol was injected into bilateral STN in rats with cortical focus. After injection, seizures were suppressed. The result suggested inhibition of the bilateral STN resulted in in activation of substantia nigra pars reticulate and seizures reduced.In experiment 3, kainic acid microinjection was performed into unilateral medial globus pallidus (GP) in rats. Each injection resulted in partial epilepsy with secondarily generalized tonic-clonic convulsions. As GP receives fibers from cerebral cortex, its excessive excitation facilitated the epileptic seizure propagation. In experiment 4, kainic acid microinjection into unilateral center-median nucleus of the thalamus (CM) resulted in partial seizures in the center-lateral limbs with secondarily generalized seizures. The results indicated that both GP and CM facilitated the epileptic seizure, both structures will be a good target to put depth electrodes for deep brain stimulations (DBS) in order to perform seizure suppression. Since DBS is less invasive treatment for intractable epilepsy patients, next step of our research is the DBS of GP and CM in kainic acid induced seizures to open clinical application of the DBS.

使用海藻酸诱导的癫痫发作模型研究了基底神经节和丘脑下核的抑制作用和兴奋作用。海藻酸的皮质内显微注射诱导皮质癫痫病。在实验1中，具有皮质焦点的大鼠，通过双侧下丘脑核（STN）的高频电刺激抑制了诱导的癫痫发作。在实验2中，将麝香酚注射到具有皮质重点的大鼠的双侧STN中。注射后，癫痫发作被抑制。结果表明，抑制双侧STN导致激活Nigra pars网状和癫痫发作。在实验3中，在大鼠中将海藻酸显微注射进行到单方面的果胶内侧球体（GP）中。每次注射都会导致部分癫痫，其次是概括性的隆隆性抽搐。由于GP从脑皮质中接收纤维，因此其过度的激发促进了癫痫发作的传播。在实验4中，海藻酸对丘脑单侧中心中心核（CM）（CM）的微分注射导致中心肢体中的部分癫痫发作，其第二个概括性癫痫发作。结果表明，GP和CM均促进了癫痫发作，这两种结构将是为深度脑刺激（DBS）提供深度电极以进行癫痫发作抑制的好目标。由于DBS对顽固性癫痫患者的侵入性较低，因此我们研究的下一步是Kainic Acid中GP和CM的DBS诱导的癫痫发作以打开DBS的临床应用。

项目成果

Clinical application of experimental cortical dysplasia in rats

大鼠实验性皮质发育不良的临床应用

• 发表时间: 2005
• 期刊: Journal of Child Neurology (in press)
• 作者: Takenaka;K.;Hasegawa;S.et al.;Tanaka T
• 通讯作者: Tanaka T

Deep brain stimulation in kainic acid-induced focal seizure

深部脑刺激在红藻氨酸诱导的局灶性癫痫发作中的应用

• 发表时间: 2004
• 期刊: Epilepsia 45(suppl.3)
• 作者: Kato I;et al.;Tanaka T
• 通讯作者: Tanaka T

ラット中大脳動脈閉塞モデルにおけるエダラボンの定位的脳内注入の効果: preliminary report

立体定向脑内注射依达拉奉对大鼠大脑中动脉闭塞模型的影响：初步报告

• 发表时间: 2004
• 期刊: 旭川てんかん外科研究 vol.III 3
• 作者: Hiroaki Osada;Hiroaki Osada;Hiroaki Osada;Hisashi Tsukada;Hisashi Tsukada;Hisashi Tsukada;Tanaka T;程塚 明;中井啓文;櫻井寿郎;橋詰清隆;Tanaka T;Hashizume K;Tanaka T;Tanaka T;Hashizume K;Tanaka T;Hashizume K;Tanaka T;Hashizume K;Tanaka T;Hashizume K;櫻井寿郎
• 通讯作者: 櫻井寿郎

飯沼一宇, 呉 繁夫, 田中達也, 佐藤康二: "けいれん発作研究の進歩-乳幼児期に生じるけいれん発作の病体と治療に関する研究 編者:杉田秀夫、高橋清久、脳科学研究の現状と課題"(株)じほう. 201-216 (2003)

Kazuu Iinuma、Shigeo Kure、Tatsuya Tanaka、Koji Sato：“惊厥性癫痫发作的研究进展 - 婴儿期惊厥性癫痫发作的病理学和治疗研究。编辑：Hideo Sugita、Kiyohisa Takahashi，脑科学研究的现状和问题” (Inc.) Jiho. 201-216 (2003)

Experimental callosotomy : Electrophysiological and Metabolic approach

实验性胼胝体切开术：电生理学和代谢方法

• 发表时间: 2004
• 期刊: Epilepsia 45(suppl.7)
• 作者: Akatsu T;Wakabayashi G;Aiura K;Suganuma K;Takigawa Y;Wada M;Kawachi S;Tanabe M;Ueda M;Shimazu M;Sakamoto M;Kitajima M.;Tanaka T
• 通讯作者: Tanaka T