Role of the mesencephalic and pontine reticular formation upon experimentally induced tonic convulsion

中脑和脑桥网状结构在实验诱发强直性惊厥中的作用

• 批准号: 08671069
• 负责人: TANAKA Tatsuya
• 金额: $ 1.28万
• 依托单位: Asahikawa Medical College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08671069/
• 关键词: Pontine reticular formation; Kainic acid; Focal seizure; Mesencephalic reticular formation; Autoradiography; Generalized tonic seizure; Pontine reticular formation; Geueralized seizure

The mode of seizure propagation was studied using generalized seizure models induced by a microinjection of kainic acid (KA) into unilateral mesencephalic reticular formation (MRF) or pontine reticular formation (PRF) of unrestrained cats or rats. Stereotaxic operation was performed in cats and a cannula for KA microinjection was implanted into MRF or PRF.One week after the surgery, KA injection was performed in freely moving condition. On EEG,a focal seizure status was initially induced at the injected site of the MRF or PRF.These seizure developed and generalized tonic seizures were induced. Clinical manifestation of MRF seizure was characterized by generalized tonic seizures followed by short clonic seizures, while that of PRF seizure was generalized tonic seizures.In rats, a cannula was implanted stereotaxically into MRF or PRF.One week after the surgery, autoradiography was performed using 14C-deoxyglucose, 131I-iomazenil or 14C-iodoantipyrine. The mode of local glucose utilization (1CGU), distribution of benzodiazepine receptors or local cerebral blood flow (1CBF) were studied. The results demonstrated that corticofugal pathway plays an important role in the generalization of MRF seizure and medial forebrain bundle plays an important role in the generalization of the PRF seizure.

本文用海人酸（KA）注射于猫或大鼠单侧中脑网状结构（MRF）或脑桥网状结构（PRF）诱发的全身性癫痫发作模型，研究了癫痫发作的传播方式。将KA微量注射套管植入MRF或PRF内，术后1周在自由活动状态下注射KA。EEG显示，MRF或PRF注射部位最初诱发局灶性癫痫发作，随后发展为全身强直性癫痫发作。MRF发作的临床表现为全身强直性发作，随后为短暂阵挛性发作，而PRF发作的临床表现为全身强直性发作，在大鼠MRF或PRF中立体定位植入套管，术后1周用14 C-脱氧葡萄糖、131 I-碘马唑尼或14 C-碘安替比林进行放射自显影。观察局部葡萄糖利用模式（1CGU）、苯二氮卓类受体分布及局部脑血流量（1CBF）。结果表明，离皮质通路在MRF发作的泛化过程中起重要作用，内侧前脑束在PRF发作的泛化过程中起重要作用。

项目成果

Tanaka T,Kunimoto M,Hashizume K,Yoshida K,Fukuda H,Tanaka S,Yonemasu Y: Basic approaches to the human complex partial seizures : Physiology, metabolism and pathology, in : The hippocampus : Functions and clinical relevance, ed : Kato N. Elsevier Science B

Tanaka T，Kunimoto M，Hashizume K，Yoshida K，Fukuda H，Tanaka S，Yonemasu Y：人类复杂部分性癫痫发作的基本方法：生理学，代谢和病理学，见：海马：功能和临床相关性，编辑：Kato N

Fujita T,: "Electroclinical and pathological studies after parenteral administration of domoic acid i freely moving non-anesthetized cats:An animal model of excitotoxicity" Journal of Epilepsy. 9. 87-93 (1996)

Fujita T，：“在自由活动的非麻醉猫中肠外施用软骨藻酸后的电临床和病理学研究：兴奋性毒性动物模型”癫痫杂志。

Tanaka T,: "Intraoperative electrocorticography in children with medically intractable epilepsy" Neurol Med Chil. 36. 440-446 (1996)

Tanaka T，：“难治性癫痫儿童的术中皮层电图检查”Neurol Med Chil。

Fukuda H,: "Petit mal-like epilepsy induced by microinjection of Baclofen(GABA agonist)into the thalamus of the rat." Epilepsia. 37 Suppl.6. 63 (1996)

Fukuda H，：“将巴氯芬（GABA 激动剂）显微注射到大鼠丘脑中诱发小癫痫样癫痫。”

Fukuda H,Tanaka T,Kunimoto M,Hashizume K,Yonemasu Y: "Petit mal-like epilepsy induced by microinjection of Baclofen (GABA agonist) into the thalamus of the rat." Epilepsia. 38 (Suppl.6). 63 (1997)

Fukuda H、Tanaka T、Kunimoto M、Hashizume K、Yonemasu Y：“将巴氯芬（GABA 激动剂）显微注射到大鼠丘脑中诱发小癫痫样癫痫。”