BASIC RESEARCH ON THE MECHANISM OF GENERALIZATION OF SEIZURES USING A BRAIN STEM FOCUS MODEL.

使用脑干焦点模型对癫痫发作泛化机制进行基础研究。

基本信息

批准号：
03807068
负责人：
TANAKA Tatsuya
金额：
$ 1.15万
依托单位：
DEPARTMENT OF NEUROSURGERY ASAHIKAWA MEDICAL COLLEGE, ASAHIKAWA 078
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1991
资助国家：
日本
起止时间：
1991 至 1992
项目状态：
已结题

项目摘要

1. Under pentobarbital anesthesia, stereotaxic operation was carried out in ten cats and a cannula was placed into the right mesencephalic reticular formation(MRF). Bipolar electrodes were placed in the MRF and the thalamus. About 8 days after recovery from the operation, 1-2 mug of kainic acid solution was injected during the animal was awake and resting. Video-EEG monitorings followed. A focal MRF seizures were initially noted at the injected site of the MRF about 30 min after the injection. However, no behavioral change was noted at this stage. These seizures reappeared every 5 minutes and developed. The seizures propagated progressively to bilateral thalamus and sensori-motor cortex (SMC). In this stage, cats demonstrated generalized tonic seizures. However, seizures became less frequent and subsided within 24 hours after the injection. The behavior of the animals also became normal afterwards. Pathological study revealed a small necrosis at the injected site of the MRF.2. Evoked potential study was made between MRF, thalamus and SMC. Rapid evoked responses (within 5 msec) were recorded at the thalamus and SMC. The results suggested that MRF played an important role in the mechanism of the secondary generalization of the seizures.3. A stereotaxic operation was carried out and a cannula was placed in the right MRF in rats. About 8 days after the surgery, kainic acid(0.5 mug) was injected into the MRF. Each injection resulted in generalized tonic seizures. An autoradiographic study using 14C-deoxyglucose was made during generalized tonic seizure status. The result disclosed hypermetabolic areas of the local cerebral glucose utilization at the injected site of the MRF as well as bilateral thalamus and SMC, which suggested immediate seizure propagation from MRF to bilateral thalamus and SMC.The results suggested a strong participation of MRF in the mechanism of generalized tonic seizure and secondary generalization of the seizures.

1。在五脑棕褐色麻醉下，在十只猫中进行了立体定位手术，并将套管放入右中脑网状形成（MRF）中。将双极电极放置在MRF和丘脑中。从手术中恢复大约8天后，在动物中注入了1-2杯的海藻酸溶液。随后进行了视频EEG监视。注射后约30分钟，在MRF注射的部位上最初在MRF癫痫发作。但是，此阶段尚未注意到行为改变。这些癫痫发作每5分钟重新出现并发展。癫痫发作逐渐传播到双侧丘脑和感觉运动皮层（SMC）。在这个阶段，猫表现出广泛的滋补性癫痫发作。但是，癫痫发作的频率降低，并在注射后24小时内消失。随后，动物的行为也变得正常。病理研究表明，在MRF的注射部位处有少量坏死。在MRF，Thalamus和SMC之间进行了诱发的潜在研究。在丘脑和SMC上记录了快速诱发的响应（5毫秒以内）。结果表明，MRF在癫痫发作的次级概括的机理中起着重要作用。3。进行了立体定位手术，并将套管放在大鼠的右MRF中。手术后约8天，将海藻酸（0.5杯）注射到MRF中。每次注射会导致普遍的补品癫痫发作。使用14C-脱氧葡萄糖的放射自显影研究是在广义曲折状态期间进行的。 The result disclosed hypermetabolic areas of the local cerebral glucose utilization at the injected site of the MRF as well as bilateral thalamus and SMC, which suggested immediate seizure propagation from MRF to bilateral thalamus and SMC.The results suggested a strong participation of MRF in the mechanism of generalized tonic seizure and secondary generalization of the seizures.