BASIC RESEARCH ON THE MECHANISM OF GENERALIZATION OF SEIZURES USING A BRAIN STEM FOCUS MODEL.

1.在戊巴比妥麻醉下，对10只猫进行了立体定向手术，并将套管置于右侧中脑网状结构（MRF）内。将双极电极放置在MRF和丘脑中。手术恢复后约8天，在动物清醒和休息期间注射1-2 μ g红藻氨酸溶液。随后进行视频脑电图监测。注射后约30分钟，在MRF注射部位最初观察到局灶性MRF癫痫发作。然而，在此阶段未观察到行为变化。这些癫痫发作每5分钟出现一次并发展。癫痫进行性蔓延到双侧丘脑和感觉运动皮层（SMC）。在这个阶段，猫表现出全身强直性发作。然而，癫痫发作变得不那么频繁，并在注射后24小时内消退。动物的行为也在之后变得正常。病理学检查显示MRF注射部位有小的坏死。在MRF、丘脑和SMC之间进行诱发电位研究。在丘脑和SMC记录快速诱发反应（5毫秒内）。结果提示，MRF在脑卒中继发性泛化的发生机制中起重要作用.在大鼠右侧MRF中进行立体定位手术并放置套管。手术后约8天，将红藻氨酸（0.5 μ g）注入MRF。每次注射都会导致全身强直性癫痫发作。在全身强直性癫痫发作状态下，使用14 C-脱氧葡萄糖进行放射自显影研究。结果显示，MRF注射部位及双侧丘脑和SMC均存在局部脑葡萄糖代谢亢进区，提示MRF可直接向双侧丘脑和SMC传播癫痫发作，提示MRF在全身强直性发作和继发性全身性发作的发生机制中有重要作用。

1. Under pentobarbital anesthesia, stereotaxic operation was carried out in ten cats and a cannula was placed into the right mesencephalic reticular formation(MRF). Bipolar electrodes were placed in the MRF and the thalamus. About 8 days after recovery from the operation, 1-2 mug of kainic acid solution was injected during the animal was awake and resting. Video-EEG monitorings followed. A focal MRF seizures were initially noted at the injected site of the MRF about 30 min after the injection. However, no behavioral change was noted at this stage. These seizures reappeared every 5 minutes and developed. The seizures propagated progressively to bilateral thalamus and sensori-motor cortex (SMC). In this stage, cats demonstrated generalized tonic seizures. However, seizures became less frequent and subsided within 24 hours after the injection. The behavior of the animals also became normal afterwards. Pathological study revealed a small necrosis at the injected site of the MRF.2. Evoked potential study was made between MRF, thalamus and SMC. Rapid evoked responses (within 5 msec) were recorded at the thalamus and SMC. The results suggested that MRF played an important role in the mechanism of the secondary generalization of the seizures.3. A stereotaxic operation was carried out and a cannula was placed in the right MRF in rats. About 8 days after the surgery, kainic acid(0.5 mug) was injected into the MRF. Each injection resulted in generalized tonic seizures. An autoradiographic study using 14C-deoxyglucose was made during generalized tonic seizure status. The result disclosed hypermetabolic areas of the local cerebral glucose utilization at the injected site of the MRF as well as bilateral thalamus and SMC, which suggested immediate seizure propagation from MRF to bilateral thalamus and SMC.The results suggested a strong participation of MRF in the mechanism of generalized tonic seizure and secondary generalization of the seizures.