Cell transplantation therapy for hemiplegia after cerebral hemorrhage

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We have developed a new model of rat spontaneous intracerebral hemorrhage. The model can be induced by intracerebra]. injection of collagenase type IV with which hemorrgae occurred in the striatum and mimicked the human intracerebral hemorrhage. The animal developed hemiplegia, reduced motion and specific rotation movement induced by methamphetamine. The rotation was correlated with amount of hemorrhage. Histological analysis indicated infiltration of macrophage/microglia in the lesion-side substantia nigra, which is followed by degeneration of the substantia nigra. In situ hybridization study indicated upregulation of Na/myoinositol cotransporter in the lesion-side substantia nigra. Degeneration of the lesion-side substantia nigra might be related to the methamphetamine-induced rotation. The brain-derived neurotrophic factor (BDNF) was indiced in the perihemorrhage striatum providing suitable condition for transplantation of the neural stem cell. We, therefore developed stem-cell transplantation study for this hemorrhage model, but transplanted-cell rate was rather low as compared to the fetal tissue transplant. We planned gene transfer to stem cell, yet we could not finish the project in this term.