Neural mechanisms of sexual motivation in female rats

条件性位置偏好(CPP)范式已被用来评估性刺激动机，以及加强药物的性质。Martinez&Paredes(2001)证明了明确的CPP只与雌性大鼠的步速交配有关。另一方面，Meerts，Christensen&Clark(2005)报告了雌性大鼠对交配相关隔室的位置偏好，无论交配是有节奏的还是无节奏的。在这项研究中，我们讨论了交配诱导的雌性大鼠CPP的这一有争议的方面。此外，几项研究揭示了性行为和药物滥用之间的关系，表明性行为和药物的强化特性存在于共同的神经基础上。在本研究中，我们研究了阿片受体拮抗剂纳洛酮对交配相关的雌性大鼠CPP完成的影响。所有女性都被切除卵巢并给予交配e-…。注射5μg苯甲酸雌二醇组(EB)和5 0 0μg孕酮组(P组)的次数较多。按2×2实验设计、配对条件(起搏或非起搏)和药物处理(纳洛酮4 mg/kg或生理盐水)将受试者分为4组(每组n=8)。每只雌性在交配前都注射了EB和P。在第1、3和5次条件训练中，所有受试者都注射生理盐水，并直接从他们的家笼子转移到他们喜欢的隔室，持续30分钟，不进行交配。在第2、4和6次条件反射实验中，受试者接受生理盐水或纳洛酮的腹腔注射。注射后一分钟，受试者被允许在有节奏或非有节奏的情况下与雄性种马交配。在接受15次入院后，立即将雌性动物放在非首选隔室中30分钟。与Meerts等人的发现一致。艾尔。(2005)，无论交配是有节奏的还是无节奏的，雌性都表现出对与交配相关的隔室的位置偏好。阿片受体拮抗剂纳洛酮阻断了起搏交配所产生的CPP。这些结果提示，起搏交配和非起搏交配可能涉及不同的性行为奖赏机制。较少

The conditioned place preference (CPP) paradigm has been used for evaluating sexual-incentive motivation, as well as reinforcing property of drugs. Martinez & Paredes (2001) demonstrated a clear CPP associated only with paced mating in female rats. On the other hand, Meerts, Christensen & Clark (2005) reported that female rats showed a place preference for the compartment associated with mating, whether mating was paced or non-paced.In this study, we addressed this controversial aspect of mating-induced CPP in female rats. In addition, several studies revealed the relationship between sexual behavior and drug abuse, suggesting that the reinforcing properties in sexual behavior and drugs lie on a common neural basis. In the present study, we examined the influence of an opioid receptor antagonist, naloxone, on accomplishment of CPP associated with mating in the female rat.Male and female Long-Evans rats (8 weeks old at start) were used. All females were ovariectomized and given mating e … More xperiences after injections of 5 μg estradiol benzoate (EB) and 500 μg progesterone (P). After that, subjects were divided into 4 groups (in each group, n=8) according to 2x2 experimental design, mating conditions (paced or non-paced) and drug treatments (4 mg /kg naloxone or saline). Each female received injections of EB and P before mating. On the 1st, 3rd, and 5th conditioning sessions, all subjects were injected saline and transferred directly from their home cage to preferred compartment for 30 min without copulation. On the 2nd, 4th and 6th conditioning sessions, subjects were injected intraperitoneally with either saline or naloxone. One min after injection, the subjects were allowed to mate with stud males in either paced or non-paced condition. Immediately after receiving 15 intromissions, the females were placed in the non-preferred compartment for 30 min. In consistent with the findings by Meerts, et. Al. (2005), females showed a place preference for the compartment associated with mating, whether mating was paced or non-paced. Opiate antagonist, naloxone, blocked the CPP induced only by paced mating.These results suggest that different reward mechanisms for sexual interaction may be involved in paced and non-paced mating. Less