Toe role of novel aggrecanase in rheumatoid arthritis and its diagnostic potential

新型蛋白聚糖酶在类风湿性关节炎中的作用及其诊断潜力

• 批准号: 15390459
• 负责人: HIROHATA Satoshi
• 金额: $ 9.6万
• 依托单位: Okayama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15390459/
• 关键词: aggrecanase; rheumatoid arthritis; matrix metalloprotease

1 Expression analysis of novel aggrecanaseRNA was extracted from IL-1 b stimulated chondrosarcoma cells and chondrocytes and the aggrecanase expressions were analyzed by quantitative RT-PCR. When compared with fibroblasts, chondrosarcoma cells and chondrocytes showed higher induction of novel aggrecanase, thus indicating this novel aggrecanase is IL-1 induced specifically in cartilage cells. When chondrosarcoma cells were stimulated with IL-1b and TNFa, novel aggrecanase induced synergistically.2 Antibody against novel aggrecanaseWe raised a polyclonal antibody against novel aggrecanase, and checked its specificity by Western blotting. A novel aggrecanase was induced by IL-1b stimulation by Western blot analysis.3 KO mouseWe got hetero mice4 Signal mechanism for novel aggrecanase inductionMAP kinase inhibitor(PD98059,SB203580) were added to IL- 1b stimulated chondrosarcoma cells and the signaling pathway was determined.

1新型聚集蛋白聚糖酶的表达分析从IL-1 B刺激的软骨肉瘤细胞和软骨细胞中提取RNA，用定量RT-PCR分析聚集蛋白聚糖酶的表达。当与成纤维细胞相比时，软骨肉瘤细胞和软骨细胞显示出更高的新型聚集蛋白聚糖酶的诱导，从而表明这种新型聚集蛋白聚糖酶是IL-1在软骨细胞中特异性诱导的。当软骨肉瘤细胞用IL-1b和TNF α刺激时，新型聚集蛋白聚糖酶协同诱导。2针对新型聚集蛋白聚糖酶的抗体我们提出了针对新型聚集蛋白聚糖酶的多克隆抗体，并通过Western blotting检测其特异性。Western blot分析IL-1b刺激诱导的一种新型聚集蛋白聚糖酶。3 KO小鼠获得异种小鼠4新型聚集蛋白聚糖酶诱导的信号机制将MAP激酶抑制剂（PD 98059，SB 203580）加入IL- 1b刺激的软骨肉瘤细胞中，检测其信号通路。

项目成果

ADAMTS9 is inducible by IL-1beta and TNF-alpha in OUMS-27 chondrosarcoma cells and in human chondrocytes.

在 OUMS-27 软骨肉瘤细胞和人软骨细胞中，ADAMTS9 可被 IL-1β 和 TNF-α 诱导。

• 发表时间: 2005
• 期刊: Arthritis & Rheumatism (印刷中)
• 作者: Demircan K

Dynamic induction of ADAMTS1 gene in the early phase of acute myocardial infarction

• DOI: 10.1093/jb/mvh138
• 发表时间: 2004-10-01
• 期刊: JOURNAL OF BIOCHEMISTRY
• 影响因子: 2.7
• 作者: Nakamura, K;Hirohata, S;Shiratori, Y
• 通讯作者: Shiratori, Y

Lp3/Hapln3, a novel link protein that co-localizes with versican and is coordinately up-regulated by platelet-derived growth factor in arterial smooth muscle cells

• DOI: 10.1016/j.matbio.2004.07.001
• 发表时间: 2004-08-01
• 期刊: MATRIX BIOLOGY
• 影响因子: 6.9
• 作者: Ogawa, H;Oohashi, T;Ninomiya, Y
• 通讯作者: Ninomiya, Y

Suppression of chondrosarcoma cells by 15-deoxy-Delta(12,14)-prostaglandin J(2) is associated with altered expression of Bax/Bcl-xL and p21.

15-脱氧-Delta(12,14)-前列腺素 J(2) 对软骨肉瘤细胞的抑制与 Bax/Bcl-xL 和 p21 表达的改变有关。

• 发表时间: 2005
• 期刊: Biochem Biophys Res Commun 328(2)
• 作者: Shen ZN

Komatsubara I: "Spatially and temporally different expression of osteonectin and osteopontin in the infarct zone of experimentally induced myocardial infarction in rats."Cardiovasc Pathol. 12・4. 186-194 (2003)

Komatsubara I：“实验诱导的大鼠心肌梗塞的梗塞区骨连接蛋白和骨桥蛋白的空间和时间差异表达。”Cardiovasc Pathol 12・4（2003）。