Inducible mechanism and role of novel aggrecanase in early stage of arthritis and its therapeutic application

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1 Inducible mechanism for aggrecanaseChondrocytes were isolated from new born rat knee joint. Mechanical stress was performed and the expressions of aggrecanases were examined. ADAMTS5 was not increased by mechanical stress, while mRNAs of ADAMTS1,4, and ADAMTS9 were increased by mechanical stress. Then, the2 Distribution of ADAMTS9 in cartilageWe produced osteoarthritis model in Wister rats. When the cartilage destruction was not so severe, the expression of ADAMTS9 was already induced. Then we examined the expression of ADAMTS9 in developmental knee joint. The knee joint was taken at 0, 7, and 14 weeks after birth in ICR mice, respectively. The expression of ADAMTS9 was observed in mature chondrocyte layer (partially) as well as hypertrophic zone. From these experiments, it is suggested that ADAMTS9 is related to the maturation of chondrocytes in knee joint.