Inducible mechanism and role of novel aggrecanase in early stage of arthritis and its therapeutic application

新型聚集蛋白聚糖酶在关节炎早期的诱导机制、作用及其治疗应用

• 批准号: 18390416
• 负责人: HIROHATA Satoshi
• 金额: $ 11万
• 依托单位: Okayama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18390416/
• 关键词: arthritis; aggrecanase; extracellular matrix

1 Inducible mechanism for aggrecanaseChondrocytes were isolated from new born rat knee joint. Mechanical stress was performed and the expressions of aggrecanases were examined. ADAMTS5 was not increased by mechanical stress, while mRNAs of ADAMTS1,4, and ADAMTS9 were increased by mechanical stress. Then, the2 Distribution of ADAMTS9 in cartilageWe produced osteoarthritis model in Wister rats. When the cartilage destruction was not so severe, the expression of ADAMTS9 was already induced. Then we examined the expression of ADAMTS9 in developmental knee joint. The knee joint was taken at 0, 7, and 14 weeks after birth in ICR mice, respectively. The expression of ADAMTS9 was observed in mature chondrocyte layer (partially) as well as hypertrophic zone. From these experiments, it is suggested that ADAMTS9 is related to the maturation of chondrocytes in knee joint.

1侵袭的诱导机制取新生大鼠膝关节，分离软骨细胞。施加机械应力，检测血管紧张素转换酶的表达。ADAMTS5不受机械应力的影响，而ADAMTS1、4和ADAMTS9的mRNAs随着机械应力的增加而增加。2 ADAMTS9在软骨中的分布我们建立了Wistar大鼠骨关节炎模型。当软骨破坏不是很严重时，ADAMTS9已经被诱导表达。然后检测ADAMTS9在发育膝关节中的表达。分别于出生后0、7、14周取ICR小鼠膝关节。ADAMTS9在成熟软骨细胞层(部分)和肥大带有表达。这些实验表明，ADAMTS9与膝关节软骨细胞的成熟有关。

项目成果

Expression of ADAMTS9 gene in chondrocytes and its regulation.

ADAMTS9基因在软骨细胞中的表达及其调控。

• 发表时间: 2007
• 作者: Saio;M.;Bai;J.;Umemura;N.;Nonaka;K.;Okada;M.;Kito;Y.;Suwa;T.;Ohe;N.;Takami;T.;Hirohata S
• 通讯作者: Hirohata S

Tumor-specific expression of the RGD-alpha3(IV)NC1 domain suppresses endothelial tube formation and tumor growth in mice

RGD-α3(IV)NC1 结构域的肿瘤特异性表达抑制小鼠内皮管形成和肿瘤生长

• 发表时间: 2006
• 期刊: FASEB 20 (11)
• 作者: Miyoshi;T.;et. al.
• 通讯作者: et. al.

Trichostatin A, a histone deacetylase inhibitor, suppresses synovial inflammation and subsequent cartilage destruction in a collagen anti body-induced arthritis mouse model

• DOI: 10.1016/j.joca.2007.10.014
• 发表时间: 2008-06-01
• 期刊: OSTEOARTHRITIS AND CARTILAGE
• 影响因子: 7
• 作者: Nasu, Y.;Nishida, K.;Ozaki, T.
• 通讯作者: Ozaki, T.

Antibodies against heat shock protein 60 derived from Helicobacter pylori:: Diagnostic implications in cardiovascular disease

• DOI: 10.1016/j.jaut.2007.05.004
• 发表时间: 2007-09-01
• 期刊: JOURNAL OF AUTOIMMUNITY
• 影响因子: 12.8
• 作者: Okada, Tomoyuki;Ayada, Kiyoshi;Oguma, Keiji
• 通讯作者: Oguma, Keiji

Coronary pressure measurement to identify the lesion requiring percutaneous coronary intervention in equivocal tandem lesions

测量冠状动脉压力以识别可疑串联病变中需要经皮冠状动脉介入治疗的病变

• 发表时间: 2006
• 期刊: Coron Artery Dis 17(2)
• 作者: Yamasaki K;Setoguchi T;Takenouchi T;Yone K;Komiya S;Sakamoto Y;Hirota M
• 通讯作者: Hirota M