Regulatory mechanism of transcriptional factors and microRNA in arthritis

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The purpose of this study was to examine the regulatory mechanism of transcriptional factors and microRNAs in chondrocyte-like cells. The analysis is important because they regulate aggrecanases that play crucial roles for osteoarthritis.In this study, we stimulated chondrosarcoma cell lines by cytokines and mechanical stress. Then the expression level of transcriptional factors and microRNAs were investigated. In the transcriptional factors, HIF-2 was not strongly induced. We further examined microRNA alteration by microRNA array. Interestingly, the microRNA expression levels were changed by cytokine stimulation as well as mechanical stress stimulation. the expressione levels of microRNAs were devided into five groups. In conclusion, the transcriptional factors and microRNAs were regulated by cytokine stimulation and mechanical stress in the distinct pathways.