Regulatory mechanism of transcriptional factors and microRNA in arthritis

转录因子和microRNA在关节炎中的调控机制

• 批准号: 23390366
• 负责人: HIROHATA Satoshi
• 金额: $ 12.23万
• 依托单位: Okayama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23390366/
• 关键词: 関節病学; アグリカナーゼ; 関節炎

The purpose of this study was to examine the regulatory mechanism of transcriptional factors and microRNAs in chondrocyte-like cells. The analysis is important because they regulate aggrecanases that play crucial roles for osteoarthritis.In this study, we stimulated chondrosarcoma cell lines by cytokines and mechanical stress. Then the expression level of transcriptional factors and microRNAs were investigated. In the transcriptional factors, HIF-2 was not strongly induced. We further examined microRNA alteration by microRNA array. Interestingly, the microRNA expression levels were changed by cytokine stimulation as well as mechanical stress stimulation. the expressione levels of microRNAs were devided into five groups. In conclusion, the transcriptional factors and microRNAs were regulated by cytokine stimulation and mechanical stress in the distinct pathways.

本研究的目的是研究转录因子和microRNAs在软骨细胞样细胞中的调控机制。这一分析很重要，因为它们调节在骨关节炎中起关键作用的聚集酶。在这项研究中，我们通过细胞因子和机械应力刺激软骨肉瘤细胞系。然后检测转录因子和microRNAs的表达水平。在转录因子中，HIF-2的诱导作用不强。我们进一步用microRNA阵列检测了microRNA的改变。有趣的是，microRNA的表达水平受到细胞因子刺激和机械应力刺激的影响。将microRNAs的表达水平分为5组。综上所述，转录因子和microRNAs在不同的途径中受到细胞因子刺激和机械应激的调节。

项目成果

Aging and CKD were the Main Risk Factors for AMI with Low LDL-C Level

老龄化和CKD是低LDL-C水平AMI的主要危险因素

• 发表时间: 2013
• 作者: Arai Y;Ueeda M;Takaishi A;Ohnishi N;Yamada D;Masuda T;Amioka N;Hirohata S;Kusachi S;Itou H
• 通讯作者: Itou H

ADAMTS1 is a novel acute biphasic marker for ischemia and reperfusion in myocardial infarction

ADAMTS1 是心肌梗塞缺血和再灌注的新型急性双相标志物

• 发表时间: 2011
• 作者: Hirohata S;Ogawa H;Miyoshi T;Obika M;Ninomiya Y;Kusachi S;Usui S;Shinohata R;Ito H
• 通讯作者: Ito H

ADAMTS1 was differently regulated in breast cancer cells

ADAMTS1 在乳腺癌细胞中受到不同的调节

• 发表时间: 2014
• 作者: Keiichi asano;Satoshi Hirohata;Masanari Obika;Omer Faruk Hatipoglu;Hiroko Ogawa;Mehmet Zeynel Cilek;Junko Inagaki;Takashi Ohtsuki;Hiroko Ishii;Kunihiko Hatanaka;Shozo Kusachi;Tomoko Yonezawa;Toshitaka Oohashi;Yoshifumi Ninomiya
• 通讯作者: Yoshifumi Ninomiya

ヒアルロン酸(HA)分子量と関節軟骨保護効果の解析(in vitro & in vivo)

透明质酸（HA）分子量和关节软骨保护作用分析（体外和体内）

• 发表时间: 2013
• 作者: 大月孝志;廣畑聡;浅野恵一;楠絵里子;稲垣純子;西田圭一郎;二宮善文
• 通讯作者: 二宮善文

リジンオリゴマーからなる関節軟骨特異的X 線プローブの創出

创建由赖氨酸低聚物制成的关节软骨特异性 X 射线探针

• 发表时间: 2014
• 作者: 大橋俊孝;芳谷 学;大野充昭;山田翔也;大月孝志;前原亜美;廣畑 聡;西田圭一郎;窪木拓男;加来田博貴;二宮善文
• 通讯作者: 二宮善文