Chemical tools for analysis and manipulation of ceramidases in human disease

用于分析和操作人类疾病中的神经酰胺酶的化学工具

• 批准号: 457290043
• 负责人: Professor Dr. Christoph Arenz
• 金额: --
• 依托单位: Arbeitsgruppe Organische und Bioorganische Chemie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 资助国家: 德国
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/457290043?language=en
• 关键词: Chemical; tools; analysis; manipulation; ceramidases

Ceramidases are enzymes that cleave the signal lipid ceramide. They play an important role in numerous physiological and pathological processes. However, their activity is difficult to analyse and there are hardly any inhibitors as biochemical tools or even as pharmacological drug candidates. In this project specific probes for individual ceramidases will be developed and validated and used to develop inhibitors for these enzymes. The suitability of these probes for flow cytometry will be tested and appropriate methods for a simple and reliable analysis of these activities will be developed in order to advance this subject of research in a significant way.

神经酰胺酶是分解信号脂质神经酰胺的酶。它们在许多生理和病理过程中起着重要作用。然而，它们的活性很难分析，几乎没有任何抑制剂作为生化工具或甚至作为药理学候选药物。在这个项目中，针对单个神经酰胺酶的特定探针将被开发和验证，并用于开发这些酶的抑制剂。将测试这些探针对流式细胞术的适用性，并开发简单可靠地分析这些活性的适当方法，以显著地推进这一主题的研究。