Multi-Parameter Flow cytometer

近年来，关于不同专业和非专业免疫细胞的表型和功能表征的日益复杂的问题已成为吕贝克大学（UzL）和石勒苏益格-荷尔斯泰因大学医院（UKSH）吕贝克校区的研究重点。流式细胞术是免疫学研究中最重要的分析方法之一，近年来由于不断的新发展，特别是在荧光染料和流式细胞仪领域，其潜力大大增加。为了成功地解决复杂的问题，需要允许进行多参数实验的细胞仪。细胞分析核心设施（CAnaCore）是UzL流式细胞术和细胞分选的技术平台，目前运行着一台带有4个激光器的分析流式细胞仪，用于检测15个颜色参数以及FSC/SSC。与此同时，多参数流式细胞仪已经开发出来，它能够同时检测30多个颜色参数，从而确定复杂的组成，以及循环和组织中免疫细胞的功能。这使得有可能，特别是有限的样本量，以获得不同来源的炎症性疾病的免疫反应的详细图片，并得出新的概念，诊断和治疗这些疾病。因此，吕贝克大学的CAnaCore将购买一台多参数流式细胞仪，这将使研究人员能够在许多不同的基础和转化研究项目中进行这种最先进的分析。这特别涉及目前DFG资助的EXC 2167“慢性炎症中的精准医学”，CRU 303“类天疱疮疾病-分子途径及其治疗潜力”，IRTG 1911“过敏和感染中炎症的免疫调节”，RTG 1743“基因，环境和炎症”，BMBF资助的德国肺部研究中心（DZL）、感染研究中心（DZIF）、心血管研究（DZHK）和糖尿病研究（DZD），以及欧盟资助的玛丽斯克洛多夫斯卡创新培训网络“补体调节和变异的寄生虫感染”（CORVOS）和ERC协同补助金“健康老化和健康的Tanycytic控制”（观看）。

In recent years, increasingly complex questions concerning the phenotypic and functional characterization of different professional and non-professional immune cells have become the focus of research at the University of Lübeck (UzL) and the University Hospital Schleswig-Holstein (UKSH) Campus Lübeck. Flow cytometry, whose potential has increased enormously in recent years due to constant new developments, especially in the field of fluorochromes and flow cytometers, is one of the most important analytical methods in immunological research. In order to successfully work on complex problems, cytometers are required which allow multiparameter experiments to be carried out. The Cell Analysis Core Facility (CAnaCore), the technology platform for flow cytometry and cell sorting at UzL, currently operates an analytical flow cytometer with 4 lasers for the detection of 15 color parameters as well as FSC/SSC. In the meantime, multiparameter flow cytometers have been developed, which are able to simultaneously detect more than 30 color parameters and thus define the complex composition and, in addition, the function of immune cells in circulation and tissues. This makes it possible, especially with limited sample quantities, to obtain a detailed picture of immune reactions in inflammatory diseases of different origins and to derive new concepts for the diagnosis and therapy of such diseases. For this reason, a multiparameter flow cytometer is to be purchased for the CAnaCore at the University of Lübeck, which will enable researchers to carry out such state-of-the-art analyses in the many different basic and translational research projects. This concerns, in particular, the current DFG-funded joint research projects within the EXC 2167 "Precision Medicine in Chronic Inflammation", the CRU 303 “Pemphigoid Diseases - Molecular Pathways and their Therapeutic Potential", the IRTG 1911 "Immunoregulation of Inflammation in Allergy and Infection", the RTG 1743 "Genes, Environment and Inflammation", the projects of the BMBF-funded networks of the German Centers for Lung Research, (DZL), Infection Research (DZIF), Cardiovascular Research (DZHK) and Diabetes research (DZD) as well as in the EU-funded Marie Sklodowska Innovative Training Network “Complement Regulation and Variations in Opportunistic Infections” (CORVOS) and the ERC Synergy Grant “Well-Aging and the Tanycytic Control of Health” (WATCH).