Multi-Parameter Flow Cytometer with High-Speed Sorting

具有高速分选功能的多参数流式细胞仪

• 批准号: 6729502
• 负责人: Alice L. Givan
• 金额: $ 39.74万
• 依托单位: DARTMOUTH COLLEGE
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-03-01 至 2005-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6729502
• 关键词: biomedical equipment purchase; flow cytometry

DESCRIPTION (provided by applicant): This Shared Instrumentation Grant application is for funding for the purchase of a Becton Dickinson FACSAria high-speed sorting flow cytometer. The instrument will be integrated into the Englert Cell Analysis Laboratory, which is a shared resource of the Norris Cotton Cancer Center at Dartmouth Medical School. The new flow cytometer will provide users with the up-to-date sorting and analysis capabilities that they require. The requirement for a new flow cytometer is based on several needs that are not met by the cytometers currently available at Dartmouth. The primary need is for the ability to define cells by more than four fluorescence parameters. The FACSAria will have three lasers and will permit users to excite a range of fluorochromes across the spectrum and to analyze and sort cells on any (or all) of thirteen fluorescence parameters. In addition, the FACSAria will allow users to save time and cell sample by the sorting of four populations of cells at one time; will allow cross-beam compensation to facilitate the use of overlapping fluorochromes; will facilitate the definition of cells with non-rectangular sort gates; and will provide for more efficient sorting at high cell flow rates. The Englert Cell Analysis Laboratory operates under the control of a Director, a Co-Director, and an Advisory Board, and with the technical support of a flow and imaging supervisor. Running costs are met by a subsidy from the NCI core grant to the Norris Cotton Cancer Center, by hourly charges to users, and by institutional support from Dartmouth Medical School. Additional institutional support will be provided by Dartmouth Medical School toward the purchase cost of the FACSAria. At the present time, there are 47 NIH grant holders dependent upon the flow cytometers in the Englert Core Laboratory; they hold, as PIs, 88 NIH grants. A "major user group" has been identified. These nine major users project 78% of the total use of the FACSAria cytometer. They hold, as PIs, NIH grants.

描述（由申请人提供）：此共享仪器补助金申请用于购买Becton Dickinson FACSAria高速分选流式细胞仪。该仪器将被集成到恩格勒细胞分析实验室，这是一个共享资源的诺里斯棉花癌症中心在达特茅斯医学院。新的流式细胞仪将为用户提供他们所需的最新分选和分析能力。对新流式细胞仪的要求是基于达特茅斯目前可用的细胞仪不能满足的几个需求。主要需要的是通过四个以上的荧光参数来定义细胞的能力。FACSAria将有三个激光器，允许用户激发光谱范围内的荧光染料，并根据十三个荧光参数中的任何一个（或全部）分析和分选细胞。此外，FACSAria将允许用户通过一次分选四个细胞群来节省时间和细胞样本;将允许交叉光束补偿以促进重叠荧光染料的使用;将促进具有非矩形分选门的细胞的定义;并且将在高细胞流速下提供更有效的分选。Englert细胞分析实验室在一名主任、一名联合主任和一个咨询委员会的控制下运作，并得到一名流动和成像主管的技术支持。运行费用由国家癌症研究所核心赠款补贴诺里斯棉花癌症中心，每小时收费的用户，并从达特茅斯医学院的机构支持。达特茅斯医学院将提供额外的机构支持，以支付FACSAria的购买费用。目前，有47个NIH补助金持有人依赖于恩格勒核心实验室的流式细胞仪;他们持有，作为PI，88 NIH赠款。已确定了一个“主要用户群体”。这九个主要用户占FACSAria细胞仪总使用量的78%。作为PI，他们持有NIH赠款。