Evolutional strategy for the establishment of angiosperm-type chloroplast NDH

我们发现一种新的蛋白质NDHS是叶绿体NDH与铁氧还蛋白高亲和力结合所必需的。叶绿体NDH不是NAD(P)H脱氢酶，而是抗霉素A抗性的铁还蛋白依赖的质醌还原酶(FQR)。我们还阐明了拟南芥中叶绿体NDH亚复合体A的组装过程，发现了这一过程所特需的几个组装因子。我们进一步发现，在Marchantia叶绿体中，NDH并不与光系统I形成超复合体，而是参与低光强下叶绿体的氧化还原动态平衡。

We discovered that a novel protein, NdhS is necessary for the high-affinity binding of chloroplast NDH with ferredoxin. Chloroplast NDH is not NAD(P)H dehydrogenase but antimycin A-resistant ferredoxin-dependent plastoquinone reductase (FQR). We also clarified the assembly process of subcomplex A of chloroplast NDH in Arabidopsis with the discovery of several assembly factors specifically required for this process. We further discovered that in Marchantia chloroplast NDH does not form the supercomplex with photosystem I and is involved in the redox homeostasis in chloroplasts at low light intensity.