Evolution and meanings of genomic wastelands (RCRO)

我们重点研究了与基因组重排密切相关的结构性异染色质(RCRO)，并对RCRO进行了分子解剖和生物学意义的估计。我们开发了一种分析重复序列的新技术，并分析了RCRO的组成和结构。特别是对黑猩猩、长臂猿和猫头猴的亚末端卫星和α卫星DNA进行了研究。在他们身上发现了令人难以置信的差异。我们发现：1)长臂猿不仅在着丝粒上有α卫星DNA，在端粒上也有α卫星DNA。2)猫头猴α卫星DNA的3种结构与染色体数目的变化有关，其中1种与夜间活动有关。3)黑猩猩允许我们假设，RCRO抑制了异位重组，使其不能与先前发现的结果进行比较。有趣的是，这些结果使我们能够意识到生物进化和染色体重组之间的一些问题。

We focused on constitutive heterochromatin (RCRO) that positively concerns to rearrangements of genome, and performed molecular dissection and estimated biological meaning of RCRO. We developed a new technique to analyze repetitive sequences and analyzed the component and construction of RCRO. Especially, subterminal satellite and alpha satellite DNA were investigated in chimpanzees, gibbons, and owl monkeys. Incredible differentiations were found in them. We found following results: 1) Gibbons have alpha satellite DNA not only at centromeres but also at telomeres. 2) Owl monkeys showed that 3 structures of alpha satellite DNA are related to change of chromosome number and one of them have some concerns with the nocturnal. 3) Chimpanzees allowed us to postulate that RCRO restrains ectopic recombinations from comparisons with results found previously. These outcomes interestingly enable us to be aware some concerns among biological evolution and chromosome reaaramgements.