Evolution and meanings of genomic wastelands (RCRO)

本论文以与基因组重排密切相关的组成型异染色质（RCRO）为研究对象，对其进行了分子解剖，并对其生物学意义进行了探讨。我们发展了一种新的技术来分析重复序列，并分析了RCRO的组成和结构。特别是在黑猩猩、长臂猿和猫头鹰猴中研究了亚末端卫星和α卫星DNA。在他们身上发现了令人难以置信的差异。结果表明：1）长臂猿的着丝粒和端粒都含有α卫星DNA。2)猫头鹰猴α卫星DNA的3种结构与染色体数目的变化有关，其中一种结构与夜行性有关。3)黑猩猩允许我们假设，RCRO抑制异位重组与以前发现的结果进行比较。有趣的是，这些结果使我们意识到生物进化和染色体重组中的一些问题。

We focused on constitutive heterochromatin (RCRO) that positively concerns to rearrangements of genome, and performed molecular dissection and estimated biological meaning of RCRO. We developed a new technique to analyze repetitive sequences and analyzed the component and construction of RCRO. Especially, subterminal satellite and alpha satellite DNA were investigated in chimpanzees, gibbons, and owl monkeys. Incredible differentiations were found in them. We found following results: 1) Gibbons have alpha satellite DNA not only at centromeres but also at telomeres. 2) Owl monkeys showed that 3 structures of alpha satellite DNA are related to change of chromosome number and one of them have some concerns with the nocturnal. 3) Chimpanzees allowed us to postulate that RCRO restrains ectopic recombinations from comparisons with results found previously. These outcomes interestingly enable us to be aware some concerns among biological evolution and chromosome reaaramgements.