Characteristics of three cytosolic glutamine synthetases and two NADH-glutamate synthase in ammonium assimilation and nitrogen utilization rice

铵同化氮利用水稻中三种胞质谷氨酰胺合成酶和两种NADH-谷氨酸合成酶的特性

• 批准号: 22248005
• 负责人: YAMAYA Tomoyuki
• 金额: $ 28.95万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-22248005/
• 关键词: イネ; 窒素利用; グルタミン代謝; 逆遺伝学; メタボローム; アンモニア同化; 窒素利用機構; グルタミン合成酵素; グルタミン酸合成酵素; 遺伝子破壊変異体; 代謝

Reverse genetics and spatial expression of genes encoding the three isoenzymes of cytosolic glutamine synthetase (GS1;1, GS1;2, and GS1;3) and two NADH-glutamatesynthases (NADH-GOGAT1 and NADH-GOGAT2) were characterized in rice. OsGS1;2 and OsNADH-GOGAT1 are important in the primary assimilation of ammonium taken up by rice roots. On the other hand, GS1;1 and NADH-GOGAT2 could be important in remobilization of nitrogen during natural senescence. Metabolite profiling data showed a crucial role of GS1;1 in coordinating metabolic balance in rice. Expression of OsGS1:3 was spikelet-specific, indicating that it is probably important in grain ripening and/or germination. Thus, these isoenzymes seem to possess distinct and non-overlapping functions and none was able to compensate for the individual function of another.

对水稻谷氨酰胺合成酶（GS 1;1、GS 1;2和GS 1;3）和两种NADH-谷氨酰胺酶（NADH-GOGAT 1和NADH-GOGAT 2）的基因进行了反向遗传和空间表达研究。OsGS 1;2和OsNADH-GOGAT 1在水稻根系吸收铵的初级同化中起重要作用。另一方面，GS1;1和NADH-GOGAT 2在自然衰老过程中的氮再动员中可能起重要作用。代谢物谱分析表明GS 1;1在协调水稻代谢平衡中起着关键作用。OsGS 1：3基因的表达具有小穗特异性，可能在籽粒成熟和/或萌发过程中起重要作用。因此，这些同工酶似乎具有不同的和不重叠的功能，没有一个能够补偿另一个的单独功能。

项目成果

Strigolactone-independent reduction of tiller number in rice mutant lacking cytosolic glutamine synthetase1 ; 2 or NADH-glutamate synthase1

缺乏胞质谷氨酰胺合成酶的水稻突变体中独立于独脚金内酯的分蘖数减少1；

• 发表时间: 2014
• 作者: Ohashi;M.;Ishiyama;K.;Hanada;A.;Kojima;S.;Hayakawa;T.;Seto;Y.;Yamaguch;Y.;Yamaya;T.
• 通讯作者: T.

Distinct function of GS1 and NADH-GOGAT isoenzymes in rice

水稻中 GS1 和 NADH-GOGAT 同工酶的独特功能

• 发表时间: 2013
• 作者: Yamaya;T.
• 通讯作者: T.

Oryza glaberrina の挿入置換系統群を用いた、イネ根長のQTL 検出と検証

利用水稻插入替代系检测和验证水稻根长QTL

• 发表时间: 2011
• 作者: 小原実広;福田善通;早川俊彦;山谷知行
• 通讯作者: 山谷知行

NADH-dependent glutamate synthase plays a crucial role in assimilating ammonium in Arabidopsis root

NADH 依赖性谷氨酸合酶在拟南芥根的铵同化中起着至关重要的作用

• 发表时间: 2014
• 期刊: Physiologia Plantarum
• 影响因子: 6.4
• 作者: Konishi;N.;Ishiyama;K.;Matsuoka;K.;Maru;I.;Hayakawa;T.;Yamaya;T. and Kojima;S.
• 通讯作者: S.

Metabolomics data reveal a crucial role of cytosolic glutamine synthetase 1;1 in coordinating metabolic balance in rice

• DOI: 10.1111/j.1365-313x.2011.04506.x
• 发表时间: 2011-05-01
• 期刊: PLANT JOURNAL
• 影响因子: 7.2
• 作者: Kusano, Miyako;Tabuchi, Mayumi;Saito, Kazuki
• 通讯作者: Saito, Kazuki