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Nitrogen Recycling System Analyzed by Molecular Physiology together with QTL Analysis in Rice

分子生理学结合QTL分析水稻氮素循环系统

基本信息

批准号：
14360035
负责人：
YAMAYA Tomoyuki
金额：
$ 10.69万
依托单位：
Tohoku University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2004
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14360035/
关键词：
rice QTL Analysis Nitrogen Recycling Chromosome-substituted lines Panicle Weight Tiller Glutamine Svnthetase DNA Markers 準同質遺伝子系統群

项目摘要

A quantitative trait locus (QTL) associated with the protein content of cytosolic glutamine synthetase (GS1 ; EC 6.3.1.2) in senescing leaves, panicle number and panicle weight was characterized in rice (Oryza sativa L.). A nearly isogenic line (NIL), C-22, developed by marker-assisted selection was grown under different nitrogen levels in a greenhouse and in a paddy field. C-22 had a small segment (approximately 50 cM) substituted from the Kasalath chromosome in chromosome 2 on a Koshihikari genetic background. C-22 showed a 12-37% lower content of GS1 protein in leaf blades than Koshihikari, which was in good agreement with a QTL region positively affected by the japonica chromosome. At an early vegetative stage, C-22 had more active tillers than Koshihikari in the greenhouse. At the reproductive stage, both panicle number and total panicle weight of C-22 were significantly higher than those of Koshihikari, particularly when grown with a low-nitrogen condition. These traits of C-22 were further confirmed in a paddy field. Thus, development of tillers was positively affected by the Kasalath chromosome at an early vegetative stage, which increased the panicle number and panicle weight at the mature stage in C-22. These data indicate that the target QTL (Pnn1 ; panicle number 1) is important in the development of tillers and panicles in rice. Pnn1 was delimited at the 6.7 cM region, demonstrating that developing tiller formation in the 2nd axil (RDT ; ratio of developing tillers formation in the 2nd axil) was determined using substituted lines. This QTL region has been successively narrowed down to 23 kb using various substituted lines of Kasalath chromosome.Physiological function of GS1 ; 1 has been investigated using knock-out mutants of GS1 ; 1 gene and this result will be published in Plant Journal. In addition, function of PII-like protein that would be expected to be involved in the Gln signaling has also been published.

在水稻（Oryza sativa L.）中表征了与衰老叶片中胞质谷氨酰胺合成酶（GS1；EC 6.3.1.2）的蛋白质含量、穗数和穗重相关的数量性状基因座（QTL）。通过标记辅助选择开发的近等基因系 (NIL) C-22 在温室和稻田的不同氮水平下生长。 C-22 在 Koshihikari 遗传背景上有一个小片段（约 50 cM）从 2 号染色体中的 Kasalath 染色体上被替换。 C-22叶片中GS1蛋白含量比Koshihikari低12-37%，这与受粳稻染色体正向影响的QTL区域非常一致。在温室的早期营养阶段，C-22 的分蘖活性比 Koshihikari 更活跃。在繁殖阶段，C-22的穗数和总穗重均显着高于越光，特别是在低氮条件下生长时。 C-22的这些特性在稻田中得到了进一步证实。因此，在营养早期，Kasalath染色体对分蘖的发育有积极的影响，这增加了C-22成熟阶段的穗数和穗重。这些数据表明目标QTL（Pnn1；穗号1）对于水稻分蘖和穗的发育很重要。 Pnn1 被界定在 6.7 cM 区域，表明使用替代系确定了第二腋中正在发育的分蘖形成（RDT；第二腋中正在发育的分蘖形成的比率）。利用Kasalath染色体的各种取代系，该QTL区域已被相继缩小至23kb。GS1的生理功能； 1 已使用 GS1 的敲除突变体进行了研究； 1基因，该结果将发表在《植物杂志》上。此外，预计参与 Gln 信号传导的 PII 样蛋白的功能也已发表。