Functions for Glutamine-Metabolizing Enzymes, Expressed in Vascular Tissues, in the Remobilization and Utilization of Nitrogen in Rice Plants

维管组织中表达的谷氨酰胺代谢酶在水稻氮再动员和利用中的功能

• 批准号: 06454070
• 负责人: YAMAYA Tomoyuki
• 金额: $ 4.48万
• 依托单位: TOHOKU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-06454070/
• 关键词: NITROGEN; GLUTAMINE SYNTHETASE; GLUTAMATE SYNTHASE; RICE(ORYZA SATIVAL); CELLULKAR LOCALIZATION; GENE EXPRESSION; NITROGEN REMOBILIZATION; RESPONSE TO NITROGEN; 維管束組織; 細胞質型グルタミン合成酵素; NADHグルタミン酸合成酵素; 免疫組織化学的解析; プロモーター領域; 遺伝子構造解析; 免疫組織学的解析; c

Cellular localization of cytosolic glutamine synthetase (GS1) protein, as well as NADH-dependent glutamate synthase (NADH-GOGAT), in vascular bundles of rice leaves have been investigated immunocytologically. The GS1 protein in senescing leaf blade was located in companion cells and vascular parenchyma cells, suggesting that the GS1 is important for the synthesis of glutamine for export of leaf nitrogen.NADH-GOGAT protein was detected in cells, which are considered to be active in the transport of solutes from phloem and xylem, in unexpanden, non-green leaf blade. This results indicates this GOGAT is important for the conversion of glutamine, transported via vascular system, to glutamate for the further use in the biosynthetic processes.In the roots, the expression of NADH-GOGAT mRNA and that of protein were greatly stimulated by the supply of nitrogen. To investigate the molecular mechanisms for the inducible increase, about 14-kb long genomic clones containing the expected promoter-region were obtained and sequenced. Agrobacterium-mediated transformation of rice plants is developed for my cultivar, Sasanishiki, to investigate the regulation of the expression.

用免疫细胞学方法研究了水稻叶片维管束中谷氨酰胺合成酶（GS 1）和依赖于NADH的谷氨酸合成酶（NADH-GOGAT）的细胞定位。GS1蛋白在衰老叶片中定位于伴胞和维管薄壁细胞中，表明GS1在叶片氮素输出的谷氨酰胺合成中起重要作用; NADH-GOGAT蛋白在未展开的非绿色叶片中定位于韧皮部和木质部溶质转运的细胞中。这一结果表明，GOGAT对通过维管系统转运的谷氨酰胺转化为谷氨酸以进一步用于生物合成过程起着重要作用。在根中，NADH-GOGAT mRNA和蛋白质的表达受到氮素供应的极大刺激。为了研究诱导性增加的分子机制，获得了含有预期启动子区域的约14-kb长的基因组克隆并测序。农杆菌介导的水稻植株转化是为我的品种，Sasanishiki，研究表达的调控。

项目成果

Yamaya, T.: Glutamine Synthetase in Nitrogon Nutrition in Higher Plants Srivastave, H.S.and Singh, R.P.Associated Publishing Co, New De1hi, 445 (1995)

Yamaya，T.：高等植物氮营养中的谷氨酰胺合成酶 Srivastave，H.S. 和 Singh，R.P. 联合出版公司，New De1hi，445 (1995)

T.Hayakawa et al.: "Cellular localization of NADH-dependent glutamate synthase protein in vascular bundles of unexpanded leaf blades and yonug grains of rice plants." Planta. 193. 455-460 (1994)

T.Hayakawa 等人：“NADH 依赖性谷氨酸合酶蛋白在水稻未展开叶片和 yonug 籽粒维管束中的细胞定位。”

Hayakawa,T.: "Cellular localization of NADH dependent glutamate synthase protein in vascular bundles of unexpanded leaf blades and young grains in rice plants." Planta. 193(3). 455-460 (1994)

Hayakawa,T.：“NADH 依赖性谷氨酸合酶蛋白在水稻未展开叶片和幼粒维管束中的细胞定位。”

Watanabe,K.: "Nucleotide sequence of cDNA encoding asparagine synthetase(Accession No.D83378)from rice callus(PGR96-20)" Plant Physiol.111(1). 347 (1996)

Watanabe，K.：“编码来自水稻愈伤组织(PGR96-20)的天冬酰胺合成酶的cDNA的核苷酸序列(登录号D83378)”Plant Physiol.111(1)。

Yamaya, T.: "Occurrence of glutamine synthetase protein associated with plasma membrane in roots of rice (Oryza sativa L.)." Physiol.Mol.Biol.Plants. 2(1). 79-82 (1996)

Yamaya, T.：“与水稻 (Oryza sativa L.) 根部质膜相关的谷氨酰胺合成酶蛋白的出现。”