课题基金 / 基金详情

Functions for Glutamine-Metabolizing Enzymes, Expressed in Vascular Tissues, in the Remobilization and Utilization of Nitrogen in Rice Plants

Functions for Glutamine-Metabolizing Enzymes, Expressed in Vascular Tissues, in the Remobilization and Utilization of Nitrogen in Rice Plants
维管组织中表达的谷氨酰胺代谢酶在水稻氮再动员和利用中的功能
批准号:
06454070
负责人:
YAMAYA Tomoyuki
金额:
$4.48万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (B)
财政年份:
1994
资助国家:
日本
项目状态:
已结题
起止时间:
1994 至 1996

项目摘要

项目成果

YAMAYA Tomoyuki的其他基金

相似基金

相关文献

中文摘要
翻译
免疫细胞学研究了水稻叶片维管束中胞质谷氨酰胺合成酶(GS1)蛋白和nadh依赖性谷氨酸合成酶(NADH-GOGAT)的细胞定位。衰老叶片中GS1蛋白主要分布在伴生细胞和维管薄壁细胞中,说明GS1对谷氨酰胺的合成和叶片氮的输出具有重要作用。NADH-GOGAT蛋白在未膨大的非绿色叶片的韧皮部和木质部的溶质运输中被认为具有活性。这一结果表明GOGAT对于谷氨酰胺的转化是重要的,通过血管系统运输,谷氨酸在生物合成过程中进一步使用。根内NADH-GOGAT mRNA和蛋白质的表达在氮的供应下受到极大的刺激。为了研究诱导增加的分子机制,获得了包含预期启动子区域的约14kb长的基因组克隆并对其进行了测序。农杆菌介导的水稻植物转化为我的栽培品种,Sasanishiki,研究表达调控。
英文摘要
Cellular localization of cytosolic glutamine synthetase (GS1) protein, as well as NADH-dependent glutamate synthase (NADH-GOGAT), in vascular bundles of rice leaves have been investigated immunocytologically. The GS1 protein in senescing leaf blade was located in companion cells and vascular parenchyma cells, suggesting that the GS1 is important for the synthesis of glutamine for export of leaf nitrogen.NADH-GOGAT protein was detected in cells, which are considered to be active in the transport of solutes from phloem and xylem, in unexpanden, non-green leaf blade. This results indicates this GOGAT is important for the conversion of glutamine, transported via vascular system, to glutamate for the further use in the biosynthetic processes.In the roots, the expression of NADH-GOGAT mRNA and that of protein were greatly stimulated by the supply of nitrogen. To investigate the molecular mechanisms for the inducible increase, about 14-kb long genomic clones containing the expected promoter-region were obtained and sequenced. Agrobacterium-mediated transformation of rice plants is developed for my cultivar, Sasanishiki, to investigate the regulation of the expression.
期刊论文(29)
专著(0)
科研奖励(0)
会议论文
Yamaya, T.: Glutamine Synthetase in Nitrogon Nutrition in Higher Plants Srivastave, H.S.and Singh, R.P.Associated Publishing Co, New De1hi, 445 (1995)
Yamaya,T.:高等植物氮营养中的谷氨酰胺合成酶 Srivastave,H.S. 和 Singh,R.P. 联合出版公司,New De1hi,445 (1995)
DOI: --
发表时间:
期刊:
影响因子: --
作者: []
通讯作者:
T.Hayakawa et al.: "Cellular localization of NADH-dependent glutamate synthase protein in vascular bundles of unexpanded leaf blades and yonug grains of rice plants." Planta. 193. 455-460 (1994)
T.Hayakawa 等人:“NADH 依赖性谷氨酸合酶蛋白在水稻未展开叶片和 yonug 籽粒维管束中的细胞定位。”
DOI: --
发表时间:
期刊:
影响因子: --
作者: []
通讯作者:
DOI: --
发表时间:
期刊:
影响因子: --
作者: []
通讯作者:
Watanabe,K.: "Nucleotide sequence of cDNA encoding asparagine synthetase(Accession No.D83378)from rice callus(PGR96-20)" Plant Physiol.111(1). 347 (1996)
Watanabe,K.:“编码来自水稻愈伤组织(PGR96-20)的天冬酰胺合成酶的cDNA的核苷酸序列(登录号D83378)”Plant Physiol.111(1)。
DOI: --
发表时间:
期刊:
影响因子: --
作者: []
通讯作者:
24
    Characteristics of three cytosolic glutamine synthetases and two NADH-glutamate synthase in ammonium assimilation and nitrogen utilization rice
    • 批准号:
      22248005
    • 项目类别:
      Grant-in-Aid for Scientific Research (A)
    • 资助金额:
      $28.95万
    • 财政年份:
      2010
    • 负责人:
      YAMAYA Tomoyuki
    • 依托单位:
    Improvement of nitrogen-use-efficiency based on molecular mechanisms of ammonium assimilation and nitrogen recycling system in rice
    • 批准号:
      19208007
    • 项目类别:
      Grant-in-Aid for Scientific Research (A)
    • 资助金额:
      $25.29万
    • 财政年份:
      2007
    • 负责人:
      YAMAYA Tomoyuki
    • 依托单位:
    Integration of nitrogen signaling and nitrogen metabolisms in rice
    • 批准号:
      16085201
    • 项目类别:
      Grant-in-Aid for Scientific Research on Priority Areas
    • 资助金额:
      $50.82万
    • 财政年份:
      2004
    • 负责人:
      YAMAYA Tomoyuki
    • 依托单位:
    Nitrogen Recycling System Analyzed by Molecular Physiology together with QTL Analysis in Rice
    • 批准号:
      14360035
    • 项目类别:
      Grant-in-Aid for Scientific Research (B)
    • 资助金额:
      $10.69万
    • 财政年份:
      2002
    • 负责人:
      YAMAYA Tomoyuki
    • 依托单位:
    海外基金